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Query: UNIPROT:P04637 (p53)
 77,613 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We established clonal cell lines from fetal brains and a cerebellum of p53 deficient mice. In this paper, stem cell lines are selectively described. FBD-103a line generates neurons, astrocytes and oligodendrocytes. 2Y-6fl line generates neurons and astrocytes. 2Y-3t line generates neurons and oligodendrocytes. FBD-104 line generates astrocytes in conventional culture, but formation of spheres in suspension culture gives the line the capability to produce neurons in addition to astrocytes. Analyses of these lines were done mainly by limited dilution cloning, immunostaining and Western blotting. Some factors in culture medium and kidney were shown to regulate differentiation of the stem cell lines.
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PMID:[Differentiation regulation of neural stem cell lines]. 1579 71

In the present study, a clonal astrocyte progenitor cell line derived from p53-deficient fetal brains, named FBD-104, was characterized in monolayer and suspension culture. In monolayer culture with medium containing 10% serum, FBD-104 cells expressed some markers of astrocytes, such as glial fibrillary acidic protein (GFAP), S100beta, and glutamate aspartate transporter (GLAST). They never expressed any markers of neurons or oligodendrocytes. Thus the cell line appears to be restricted to the astroglial lineage. However, in suspension culture in serum-free medium supplemented with EGF and FGF2, FBD-104 cells proliferated and formed neurospheres expressing mRNAs for Mash1 and Math3, generating cells expressing neuron specific beta-III tubulin. Re-plating the spheres onto an adhesive substrate and withdrawal of the growth factors induced the expression of mRNAs for NeuroD and Olig2 and generated more beta-III tubulin-positive cells. The present study demonstrated that neurosphere culture is an efficient method to induce neurogenesis from the astrocyte progenitor cell line FBD-104. We also determined that pretreatment with FGF2 caused a significant increase in yield of neurospheres. Thus, the FBD-104 line is an interesting in vitro model to study effect of trophic factors and adhesive substrates on lineage determination of neural progenitor cells.
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PMID:An attempt to generate neurons from an astrocyte progenitor cell line FBD-104. 1605 58

We previously established cell lines from brains of p53-deficient embryos, and have now characterized one of these lines, FBD-103a, in detail. Recloning FBD-103a yielded 3 types of subclones: 5 generated the neuronal lineage (Type 1), 3 generated the glial lineage (Type 2), and 10 gave rise to both lineages as the parental line (Type 3), indicating that FBD-103a is a multipotent neural progenitor cell line indistinguishable from true neural stem cells. RT-PCR analyses of transcription factor expression indicated that the transition of multipotent Type 3 clones to either neuronally or glially differentiated progeny was marked by down-regulation of Ascl1/Mash1 and Olig1 and up-regulation of Nrsf/Rest. As expected for neural stem cells, FBD-103a and Type 3 clones formed neurospheres when cultured on a non-adhesive substrate in a serum-free medium containing fibroblast growth factor-2 (FGF2). Interestingly, the transition between Type 3 and Type 1 neuronal- or Type 2 glial-specified cells proved to be reversible; Type 1 and Type 2 subclones could also form neurospheres, from which both neuron-generating and glia-generating progenies could be derived. Moreover, when maintained on an adherent substratum that prevented neurosphere formation, but with FGF2 and without serum, Type 2 clones could generate Type 3 multipotent cells. Thus, at least in the absence of p53, specialized cell-cell interactions within neurospheres are not essential for persistence or recapture of the capacity for self-renewal and multipotency by cells differentiating along glial pathways, a result of possible significance to the pathogenesis of malignant brain tumors.
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PMID:Multipotency of FBD-103a, a neural progenitor cell line from the p53-deficient mouse. 1633 44

Interactions between oligodendroglial and astroglial lineages during development are still unclear. In this study, FBD-102b, derived from p53-/- fetal brains, was characterized as an oligodendroglial progenitor cell (OPC) line. In co-culture with 102b cells, cells of an astrocyte progenitor cell line, FBD-104, elongated monopolar processes, like radial glia, and actively proliferated. Conditioned medium of 102b cells also induced the process elongation that was associated with down-regulation of GFAP and up-regulation of vimentin and nestin. These results suggested that OPCs secrete a radial glia-inducing factor that might maintain the radial glial processes to use as scaffolds of migration.
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PMID:An oligodendroglial progenitor cell line FBD-102b possibly secretes a radial glia-inducing factor. 1688 1