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Query: UNIPROT:P04626 (erbB-2)
 5,251 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cellular differentiation and mRNA levels of genes involved in kidney growth were investigated in normal kidney cells, cyst-lining epithelial cells of polycystic kidney disease, and renal carcinoma cells (RCC). All cells comparatively studied exhibited an antigenic phenotype of proximal tubular cells as shown by the expression of a panel of brush border membrane enzymes and kidney-associated cell surface antigens. The epithelial developmental antigen Exo-1 was expressed in 50% to 80% of cyst-lining epithelia in polycystic kidney tissue and in 20% to 30% of polycystic kidney cells cultured in vitro. Normal kidney cells and RCC were negative under identical culture conditions. The expression of antigen Exo-1 is associated with hyperproliferation in an epithelial tissue compartment composed of cells which have not yet reached their terminal differentiation state. Increased amounts of mRNA of the growth factor receptor system of epidermal growth factor (EGF) receptor and its ligand transforming growth factor (TGF)-alpha were associated with the malignant phenotype of RCC. Increased expression of EGF receptor and TGF-alpha, although less prominent, were also observed in polycystic kidney cells compared with normal kidney cells. In conclusion, the expression of Exo-1 in cyst-lining epithelial cells of autosomal dominant polycystic kidney disease (ADPKD) and the altered regulation of TGF-alpha and EGF receptor in these cells contribute to the hypothesis that hyperproliferation is an underlying pathogenic mechanism of ADPKD.
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PMID:Expression of differentiation antigens and growth-related genes in normal kidney, autosomal dominant polycystic kidney disease, and renal cell carcinoma. 173 78

In a previous paper, we have shown that the c-erbB-2-encoded protein p185erbB2 is localized on the brush border of the proximal tubule kidney cells. In invasive duct cell carcinomas, the labeling was most obvious on the villous plasma membrane protrusions. From these observations the hypothesis was raised that p185erbB2 could play a role in motility. To test this hypothesis, we quantified its distribution on the microvilli and plasma membrane protrusions and on the straight parts of the cell membrane after immunoelectron microscopy. These findings were compared with the localization on p185erbB2 overexpressing SK-BR-3 human breast cancer cells before and after stimulation of motility by treatment with conditioned medium from COLO-16 cells (CM), which is also able to induce chemotaxis of these cells in a Boyden chamber assay. In the invasive duct cell carcinomas, the number of gold particles was nine times higher at the plasma membrane protrusions than at the straight parts of the cell membrane. In untreated SK-BR-3 cells, p185erbB2 was similarly concentrated on the membrane of small microvilli and plasma membrane protrusions. Treatment of SK-BR-3 cells with CM leads to cell spreading, enlargement of the microvilli, formation of pseudopodia and chemotaxis. Aggregation of p185erbB2 at the plasma membrane protrusions and pseudopodia is observed on immunofluorescence light microscopy. The concentration of p185erbB2 is several times higher on these membrane extensions than on the straight parts after immunogold labeling. It is concluded that p185erbB2 is localized on cell organelles involved in motility, and it is suggested that the molecule plays a role in cell movement, providing the capacity of tumor cells to spread and metastasize.
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PMID:The p185erbB2 protein is localized on cell organelles involved in cell motility. 790 Sep 45