Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P04626 (erbB-2)
 5,251 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Interleukin (IL) 1 alpha induced the up-regulation of cell-surfac-expressed epidermal growth factor (EGF) receptor on both MDA-MB-468 and BT-20 breast cancer cell lines. IL-1 beta and tumor necrosis factor alpha (TNF-alpha) increased the EGF receptor surface expression only on BT-20 breast carcinoma cells. 12-O-tetradecanoylphorbol 13-acetate (TPA) induced up-regulation of EGF receptor on MDA-MB-468 cells, and a marginal but significant increase was determined in BT-20 cells and in interferon gamma (IFN-gamma)-treated MDA-MB-468 cells. CD15 (Lewisx) antigen was down-regulated on MDA-MB-468 cells by TNF-alpha, TPA, IL-1 alpha, as well as by IFN-gamma and all-trans-retinoic acid. 1,25(OH)2-vitamin D3 up-regulated the CD15 antigen surface expression on MDA-MB-468 cells.
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PMID:Modulation of EGF receptor and CD15 (Lewisx) antigen on the cell surface of breast carcinoma cell lines induced by cytokines, retinoic acid, 12-O-tetradecanoylphorbol 13-acetate and 1,25(OH)2-vitamin D3. 790 17

DNA topoisomerase I (topo I) is the molecular target of the camptothecin group of anticancer drugs. These drugs are S-phase specific and require elevated topo I for tumor cell killing. To determine whether increased topo I expression occurs in testicular seminomas, 20 cases of testicular seminoma were retrieved from the surgical pathology files at the University of Utah Health Sciences Center and stained with an antibody that recognizes topo I in paraffin embedded human tissue sections. Topo I elevation was observed in 30% (6/20) of the seminomas. Because the response to topo I targeted drugs requires cell proliferation, the proliferative index of the seminomas was determined by immunohistochemical staining for DNA topoisomerase II-alpha (topo II-alpha) a new marker of cell proliferation. AU seminomas had easily detectable topo II-alpha. The average topo II-alpha index of the 20 cases was 52.1 +/- 15.3. Seminomas with elevated topo I had an average topo II-alpha proliferative index of 60.8 +/- 17.5 and seminomas with normal topo I expression had a topo II-alpha proliferative index of 48.4 +/- 13.2. This is significantly different at the 0.05% confidence level. Focal expression of CD30 was seen in 60% (12/20) of the neoplasms. None of the cases showed positive staining for CD15 and c-erbB-2. Our results suggest that chemotherapeutic protocols involving topoisomerase targeting drugs might be useful against testicular seminomas.
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PMID:Expression of DNA toposiomerase I and DNA topoisomerase II-alpha in testicular seminomas. 1087 53