Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P04626 (erbB-2)
 5,251 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of trastuzumab on patients with HER-2/neu (HER2)-positive gastric cancer has been confirmed in a phase III clinical trial (ToGA study). However, the optimized sequence and synergic mechanism of trastuzumab and chemotherapy are not clear. Our study investigated the effects and mechanisms of trastuzumab in combination with 5-Fluorouracil (5-Fu) or cisplatin (DDP) on gastric cancer cell lines. Flow cytometry was used to determine HER2 expression and cell cycle. MTT assay was performed to evaluate cytotoxicity. Western blotting and RT-PCR were used to analyze signaling transduction and mRNA expression. Sequential 5-Fu followed by trastuzumab and trastuzumab plus DDP followed by trastuzumab produced the best inhibitory effects. Inhibition of HER2-PI3K-AKT signal transduction, downregulation of nucleotide excision repair cross-complementation 1 (ERCC1), and interference with cell cycle distribution may elucidate the synergism between trastuzumab and chemotherapy. These results provide some evidence for designing a rational regime when trastuzumab is being considered to be used in patients with gastric cancer.
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PMID:The sequence-dependent cytotoxic effect of trastuzumab in combination with 5-Fluorouracil or cisplatin on gastric cancer cell lines. 2059 Apr 42

The function of human epidermal growth factor receptor 2 (HER2) in the chemosensitivity of ovarian carcinoma has not been fully investigated, therefore, the present study aimed to analyze the potential role of HER2 in ovarian carcinoma chemosensitivity in further detail. SKOV3 cells were transfected with lentiviral-mediated HER2-small hairpin RNA (shRNA) molecules to establish the stable expression of HER2-shRNA in the SKOV3 cell line (knockdown cells; KD) and negative control cell line (NC). The untransfected SKOV3 cell line served as the blank control (CON) group. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis were used to detect the expression of HER2 in the three different cell types, which were subsequently examined for growth inhibition using a cell counting kit-8 assay. The CON and KD cell strains were utilized to establish xenograft models in nude mice, primarily to detect the expression of the HER2 protein, and additionally to observe tumor size changes under the treatment of cisplatin (DDP) chemotherapy. RT-qPCR and western blot analysis demonstrated a significant decrease in the levels of HER2 mRNA and protein in the KD cells. The suppression of HER2 expression resulted in an increase of chemotherapy sensitivity in the SKOV3 cells. HER2 protein expression decreased significantly following transduction with specific HER2-shRNA. Additionally, growth slowed significantly under treatment with DDP in ovarian cancer transplantation tumors. In conclusion, lentivirus-mediated HER2-shRNA effectively inhibits the expression of the HER2 gene, and increases the chemosensitivity to DDP in ovarian carcinoma.
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PMID:Suppression of human epidermal growth factor receptor 2 via interference increases the chemosensitivity of ovarian carcinoma. 2712 58

Although many breast cancer cell lines have been used for cancer research during the past several decades, few have originated from primary tumors in Asian patients. Moreover, the incidence of breast cancer has been increasing rapidly in China during this time period. Therefore, it is essential to establish breast cancer cell lines from Chinese patients. Here, we report the establishment of three new breast cancer cell lines, designated BC-023, BC-024, and BC-034, from breast carcinoma tissues of three Chinese patients. These breast cancer cell lines grew as adherent monolayers with characteristic epithelial morphology and were maintained continuously in vitro with stable growth rates for at least 20 passages. No bacterial, fungal, or mycoplasma contamination was detected in any of the three cell lines. Additionally, these cells were human epidermal growth factor receptor 2 (C-erbB-2)-positive. All three cell lines had comparable population doubling times of 33-39 h and reproducibly formed colonies in soft agar. Furthermore, these cells displayed aggressive tumorigenicity. Thus, every characteristic of each of these cell lines meets the quality control standards of the American Type Culture Collection (ATCC). We used drug sensitivity testing with growth-inhibition assays and showed that these three lines expressed a wide range of sensitivities to cisplatin (DDP) and adriamycin (ADR). These studies indicate that these three novel cell lines may provide new models for studying cancer biology and for screening new drugs for breast cancer treatment, especially for the Chinese population.
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PMID:Novel cancer cell lines derived from primary breast tumors in Chinese patients. 3066 42