Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P04179 (MnSOD)
 2,777 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Using DNA microarray analysis, we found that human macrophages respond to oxidized low-density lipoprotein (oxLDL) by activating the antioxidative glutathione and thioredoxin systems. Several genes of the glutathione and thioredoxin systems were expressed at high levels in macrophages when compared to 80 other human tissues and cell types, indicating that these systems may be of particular importance in macrophages. The up-regulation of three genes in these systems, thioredoxin (P < 0.005), thioredoxin reductase 1 (P < 0.001) and glutathione reductase (P < 0.001) was verified with real-time RT-PCR, using human macrophages from 10 healthy donors. To investigate the possible role of these antioxidative systems in the development of atherosclerosis, expression levels in macrophages from 15 subjects with atherosclerosis (12 men, 3 women) and 15 matched controls (12 men, 3 women) were analyzed using DNA microarrays. Two genes in the glutathione system Mn superoxide dismutase (P < 0.05) and catalase (P < 0.05) differed in expression between the groups. We conclude that macrophage uptake of oxidized LDL induces a coordinated up-regulation of genes of the glutathione and thioredoxin systems, suggesting that these systems may participate in the cellular defense against oxidized LDL and possibly modulate the development of atherosclerosis.
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PMID:Oxidized LDL induces a coordinated up-regulation of the glutathione and thioredoxin systems in human macrophages. 1604 14

Bleomycin (BLM) is an anti-cancer drug that can induce formation of reactive oxygen species (ROS). To investigate the association between up-regulation of antioxidant enzymes and coenzyme Q(10) (CoQ(10)) in acquired BLM resistance, one BLM-resistant clone, SBLM24 clone, was selected from a human oral cancer cell line, SCC61 clone. The BLM resistance of SBLM24 clone relative to a sub-clone of SCC61b cells was confirmed by analysis of clonogenic ability and cell cycle arrest. CoQ(10) levels and levels of Mn superoxide dismutase, glutathione peroxidase 1, catalase and thioredoxin reductase 1 were augmented in SBLM24 clone although there was also a mild increase in the expression of BLM hydrolase. Suppression of CoQ(10) levels by 4-aminobenzoate sensitized BLM-induced cytotoxicity. The results of suppression on enhanced ROS production by BLM and the cross-resistance to hydrogen peroxide in SBLM24 clone further demonstrated the development of adaptation to oxidative stress during the formation of acquired BLM resistance.
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PMID:Up-regulation of antioxidant enzymes and coenzyme Q(10) in a human oral cancer cell line with acquired bleomycin resistance. 2148 14

Silver nanoparticles (AgNPs) are extensively used in many commercial products because of their antimicrobial properties and they are therefore released into the environment from various products. A number of genes, especially those representing antioxidant and detoxification pathways, have potential application for studying mechanism of action of environmental pollutants at molecular level. In the present study, the stress responsive transcription of antioxidant and detoxification genes in response to AgNPs and Ag(+) ions exposure is studied in the ecotoxicologically important model species Chironomus riparius. The selected genes were superoxide dismutases (CuZnSOD and MnSOD), catalase (CAT), phospholipid hydroperoxide glutathione peroxidase 1 (PHGPx1), thioredoxin reductase 1 (TrxR1), and delta-3, sigma-4 and epsilon-1 classes of glutathione S-transferases (GSTs). The mRNA expression levels of each gene were determined after exposure of animals for 24h to three different AgNP and Ag(+) ion concentrations using Real-Time PCR method. Significant up-regulation of CuZnSOD and MnSOD was found after exposure to Ag(+) ions and AgNPs, respectively. The transcript levels of CAT, PHGPx1 and TrxR1 were significantly up-regulated only after exposure to AgNPs and no significant change was observed after exposure to Ag(+) ions. The expression levels of all the GSTs were more pronounced after exposure to AgNPs as compared to Ag(+) ions. The overall results suggest that AgNPs led to pronounced induction of genes related to oxidative stress and detoxification than Ag(+) ions.
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PMID:Evaluation of the effect of silver nanoparticles and silver ions using stress responsive gene expression in Chironomus riparius. 2366 72