Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P04179 (
MnSOD
)
2,777
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The mechanism(s) of the "aldosterone turn-off phenomenon", hypoaldosteronemia following chronic ACTH administration, remains unclear. Our previous observation that antioxidants prevented turn-off prompted us to evaluate the chronic effect of ACTH on the enzymatic antioxidant system as well as P450aldo activity and expression of
CYP11B2
in adrenal zona glomerulosa. Male Wistar rats were administered ACTH-Z for 5 days with or without antioxidants, vitamin E or DMSO. Adrenal capsules were prepared for P450aldo activity measurement and mRNA content determination by competitive RT-PCR, and immunoreactivity of
Mn-SOD
in whole adrenals was evaluated. ACTH decreased the P450aldo activity and mRNA level of
CYP11B2
in adrenal capsules, while co-administration of vitamin E or DMSO partially blocked this inhibition. ACTH increased
Mn-SOD
mRNA and immunoreactivity but decreased GPx mRNA. These results suggest that prolonged ACTH treatment increases oxidative stress in the zona glomerulosa and an imbalance in the ratio of
Mn-SOD
to GPx, possibly via corticosterone overproduction in the zona fasciculata, resulting in the downregulation of
CYP11B2
. Vitamin E and DMSO might thus protect
CYP11B2
expression through their antioxidant actions.
...
PMID:Role of rat adrenal antioxidant defense systems in the aldosterone turn-off phenomenon. 1082 27
