Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P04179 (MnSOD)
 2,777 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

It has been documented that C6 glioma cells can be changed into normal glial cells when they were cultured in serum free medium. In the present study, the expressions of inducible nitric oxide synthase (iNOS) and superoxide dismutase (SOD) were investigated. The mRNA level of iNOS was markedly increased by lipopolysaccharide (LPS) in cultured rat brain astrocytes (RBA-1) but not in C6 glioma cells. However, increase of mRNA for iNOS by LPS can be obtained in C6 glioma cells when they were cultured in serum free medium. The mRNA level of magnesium-SOD (Mn-SOD) was increased by LPS in both cells while the expression of constituted SOD (Cu,Zn-SOD) was not stimulated by LPS. Western blotting analysis indicating the amount of protein showed a similar change. After serum deprivation, the protein of iNOS or Mn-SOD was increased by LPS in C6 glioma cells in a way similar as that in RBA-1 cells. These results suggest that serum free conditioned C6 glioma cells were adapted to astroglial cell-like properties which may express more iNOS and Mn-SOD mRNA in the presence of LPS.
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PMID:Effect of serum in medium on the expression of inducible nitric oxide synthase and superoxide dismutases in cultured C6 glioma cells. 1008 21

Reactive oxygen species have been linked with neuropathological changes in the central nervous system. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Superoxide dismutase (SOD) is an endogenous enzyme which can scavenge reactive oxygen species. This study investigated the effect of supplementation with ascorbic acid (vitamin C) on the changes of SOD in cultured neurological cells. Rat brain astrocytes (RBA-1 cells) were incubated with vitamin C and divided into four groups: a control group (without vitamin C) and three treatment groups with vitamin C at 40, 80, and 160 micromol/l. After short-term (2 days) and long-term (7 days) incubation, SOD activity, SOD mRNA level by Northern blotting, and SOD protein amounts by Western blotting were measured. After 2 days of incubation, vitamin C resulted in a decrease in the activity of SOD in a concentration-dependent manner (Mn-SOD from 14.8 +/- 1.2 to 13.2 +/- 0.5 U/mg protein and Cu/Zn-SOD from 64.8 +/- 1.2 to 51.7 +/- 0.9 U/mg protein; p < 0.05), and vitamin C also attenuated the Cu/Zn-SOD mRNA level from 100 to 86.3 +/- 6.7%; p < 0.01), whereas the protein amounts of these two SODs remained unchanged. After 7 days of incubation with vitamin C, the SOD activity of RBA-1 cells decreased significantly (Mn-SOD from 14.9 +/- 0.3 to 11.8 +/- 0.3 U/mg protein and Cu/Zn SOD from 61.8 +/- 1.8 to 54.6 +/- 0.9 U/mg protein; p < 0.01), and the mRNA level was also attenuated (Mn-SOD from 100 to 86.8 +/- 8.7% and Cu/Zn-SOD from 100 to 84.7 +/- 4.8%; p < 0.01). These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may downregulate activity and gene expression of SOD in cultured RBA-1 cells.
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PMID:Downregulation of superoxide dismutase activity and gene expression in cultured rat brain astrocytes after incubation with vitamin C. 1288 23