Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P04179 (MnSOD)
 2,777 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

At least five different transgenic approaches have been applied to the study of Drosophila aging. There are two single component systems: transgenes with native (normal) promoters and transgenes with heterologous promoters; as well as three binary systems: 'GAL4/UAS', 'FLP-out' and 'tet-on'. These approaches vary in ability to meet several technical challenges, and the relative advantages and disadvantages of each are discussed. Using these techniques, over-expression of the hsp70, Cu/ZnSOD and MnSOD genes has each been demonstrated to increase Drosophila life span.
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PMID:Transgenic methods for increasing Drosophila life span. 1098 20

A transgenic system ("FLP-out") based on yeast FLP recombinase allowed induced overexpression of MnSOD enzyme in adult Drosophila melanogaster. With FLP-out a brief heat pulse (HP) of young, adult flies triggered the rearrangement and subsequent expression of a MnSOD transgene throughout the adult life span. Control (no HP) and overexpressing (HP) flies had identical genetic backgrounds. The amount of MnSOD enzyme overexpression achieved varied among six independent transgenic lines, with increases up to 75%. Life span was increased in proportion to the increase in enzyme. Mean life span was increased by an average of 16%, with some lines showing 30-33% increases. Maximum life span was increased by an average of 15%, with one line showing as much as 37% increase. Simultaneous overexpression of catalase with MnSOD had no added benefit, consistent with previous observations that catalase is present in excess in the adult fly with regard to life span. Cu/ZnSOD overexpression also increases mean and maximum life span. For both MnSOD and Cu/ZnSOD lines, increased life span was not associated with decreased metabolic activity, as measured by O2 consumption.
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PMID:Induced overexpression of mitochondrial Mn-superoxide dismutase extends the life span of adult Drosophila melanogaster. 1207 63

The FLP-out technique, based on yeast FLP recombinase, allows induced over-expression of transgenes in Drosophila adults. With FLP-out control and over-expressing flies have identical genetic backgrounds and therefore differences in life span must result from transgene induction. The amount of over-expression achieved varies between independent transgenic lines, and previously for both Cu/ZnSOD and MnSOD life span was found to be increased in proportion to the increase in enzyme activity. To determine if greater increases in enzyme and life span could be achieved with FLP-out, enzyme over-expression and life span were analyzed in eight lines containing two MnSOD transgenes, three lines containing three MnSOD transgenes, and three lines containing a MnSOD transgene plus a Cu/ZnSOD transgene. Life span was again found to be increased in proportion to the increase in MnSOD enzyme activity, with increases of up to 40% in mean and maximum life span. However the increases in enzyme activity and life span conferred per transgene were reduced when more than one transgene was present at the same time. When the reduced efficiency of enzyme over-expression per transgene was taken into account, simultaneous over-expression of MnSOD and Cu/ZnSOD was found to have partially additive effects on life span.
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PMID:Effects of simultaneous over-expression of Cu/ZnSOD and MnSOD on Drosophila melanogaster life span. 1513 Jul 51