Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P04040 (
Catalase
)
3,577
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Catalase
(
CAT
) in solution or incorporated in erythrocytes and K562 leukemic cells is inactivated during photosensitization with tetrasulfonated metallophthalocyanines (MePcS4). The effect of added scavengers and D2O showed that both singlet oxygen and free radical species are involved in this process. Evidence was found that direct interactions of ground or excited-stated photosensitizer with
CAT
are not responsible for
CAT
inactivation. Specific techniques to probe early damage to the
CAT
structure involved optical and EPR spectroscopy, HPLC and polyacrylamide gel electrophoresis analyses. Different primary events of photosensitized protein damage included oxidation of cysteine residues as well as other amino acids, as demonstrated by the formation of carbon-centered free radicals and the loss of absorbance at lambda = 275 nm. In parallel, we detected degradation of the
CAT
heme groups, accompanied by release of Fe(II) ions in solution. These combined phenomena initiate cross-linkages between
CAT
subunits and subsequent degradation of the protein with formation of irreversible aggregates in solution.
Phthalocyanine
-mediated photoinactivation of cell-bound
CAT
results in loss of protection against accumulating H2O2, providing an additional pathway of phototoxicity.
...
PMID:Catalase inactivation following photosensitization with tetrasulfonated metallophthalocyanines. 763 56
