Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P04040 (Catalase)
3,577 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Ribulose-1,5-bisphosphate carboxylase/oxygenase, catalase, glycolate oxidase, and hydroxypyruvate reductase activities on a protein and fresh weight basis were measured over seven stages of tomato fruit development and ripening. Ribulose-1,5-bisphosphate carboxylase decreased steadily during fruit development from 23 +/- 8 nmoles per minute per milligram protein at the mature green stage to 13.4 +/- 2 at the table ripe stage. There was no change in partially purified preparations of the enzyme in the ratio of carboxylase to oxygenase activity, which was about 10. Catalase activity reached a maximum during the climacteric, simultaneously with increased ethylene and CO(2) formation. Glycolate oxidase activity decreased during early stages of development and was barely detectable at the climacteric. Hydroxypyruvate reductase, associated with serine formation by the glycerate pathway, increased in specific activity during early stages of tomato fruit ripening. In the fruit of the rin tomato mutant, which does not ripen normally, none of these changes in enzyme activity occurred.
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PMID:Changes in Activity of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase and Three Peroxisomal Enzymes during Tomato Fruit Development and Ripening. 1666 Jul 53

The crude homogenate of cells from Chlamydomonas reinhardii was placed on a linear gradient from 30% to 60% sucrose and centrifuged for 4 hours at 60 000 g. After fractionation of the gradient the distribution of enzymes was determined. Hydroxypyruvate reductase and glycolate dehydrogenase, two markers for peroxisomes, appeared with one sharp peak at density 1.185 g/cm(3) within the gradient. Twenty-five percent of the hydroxypyruvate reductase was particulate and 75% was found as solubles in the top fractions. The peaks of both enzymes matched exactly the peak of the cytochrome oxidase which is a marker for mitochondria. The profile for malate dehydrogenase was the same as that for hydroxypyruvate reductase. Catalase, however, showed two peaks. One coincided with the peak of cytochrome oxidase and the other appeared at density 1.22 g/cm(3). More than 50% of the catalase moved into the gradient during centrifugation.
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PMID:[Distribution of microbody enzymes from Chlamydomonas on sucrose gradients]. 2444 97