Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P04040 (
Catalase
)
3,577
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effect of advanced glycation end products (AGE-s) was studied on the proliferation and cell death of human skin fibroblasts in culture. Several
AGE
-products were prepared from proteins, a peptide and amino acids, using Glucose or Fructose, with or without Fe2+. The
AGE
preparations increased cell death at the 7th day, after only 72 hours of incubation. Some of these glycation products modified also proliferation. This effect of
AGE
-s was even maintained without these products in fresh medium for a second period of incubation up to 10 days from the start of the experiment. In order to explore the role of
AGE
-receptors, especially of
AGE
-receptor and of growth factor receptors (fibroblast and epidermal growth factors receptors), antibodies to these receptors were added to cell cultures and their effect on both cell death and proliferation were determined as for the
AGE
-s. These anti-receptor antibodies imitated to some extent the results obtained with
AGE
-s, producing increase of cell death and proliferation, followed above a certain concentration of antibodies by a decrease and a new increase or plateau. This might correspond to the internalization of the receptors followed by a re-expression on the cell membrane. The role of receptor-mediated Reactive Oxygen Species-production was also explored using scavengers: N-acetyl-cysteine (NAC), L-Carnosine, superoxide dismutase (SOD) and
Catalase
. Several of these scavengers decreased cell death, suggesting that Reactive Oxygen Species-production is partially involved in the observed phenomena.
...
PMID:Effect of advanced glycation end-products on cell proliferation and cell death. 1691 94
Advanced Glycation End-products (AGE-s) were shown to exhibit a number of potentially harmful properties in contact with cells and tissues. As their concentrations increases with age, faster even in hyperglycemic individuals, they are considered important for aging- and age-associated pathologies, especially for athero-arteriosclerosis and type II diabetes. We describe here the methods used for the demonstration of a direct cytotoxicity of several
AGE
-products when added to human skin fibroblast cultures. This cytotoxicity was still demonstrable when cells, previously cultured with
AGE
-s, were transferred to new medium without
AGE
-s. This effect, the remanence of cytotoxicity in absence of
AGE
-s, suggests a certain degree of inheritance, possibly by epigenetic mechanisms, of the cytotoxic effect of
AGE
-s, mediated by the
AGE
-receptors (RAGE-s) and inhibited by free radical-scavengers, such as L-Carnosine,
Catalase
and Rhamnose-rich oligo- and polysaccharides. Such cytotoxicity can occur not only on the skin but also in other tissues. It appears thus that besides the crosslinking of collagen and other macromolecules, the products of the Maillard reaction can exert their harmful cytotoxic effects directly on the cells.
...
PMID:[Demonstration of the cytotoxic effect of Advanced Glycation Endproducts (AGE-s)]. 1797 52
