Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A procedure has been developed for the immunoelectron microscopic localization of intracellular antigens on thin-sectioned tissues. The tissues were fixed in a periodate-lysine-paraformaldehyde solution or a formaldehyde-glutaraldehyde combination and embedded in the acrylate-methacrylate mixture, Lowicryl K4M (Polaron), which was polymerized under ultraviolet irradiation at -35 degrees C. Thin sections were mounted on gold grids, immunostained using an indirect method with ferritin-labeled antibodies, and, optionally, counterstained with osmium tetroxide and/or lead citrate and uranyl acetate. The procedure provided good morphologic preservation of the cell architecture in adult and embryonic heart, and skeletal and smooth muscle tissue, as well as nonmuscle cells. At the same time it retained the antigenicities of several contractile proteins, including myosin, tropomyosin, actin, and alpha-actinin. The method has advantages over en bloc staining techniques in that the problem of antibody penetration into the cells is eliminated and careful controls can be performed on adjacent sections. This technique will be useful for localizing, at the ultrastructural level, contractile and other selected proteins in a variety of muscle and non-muscle cells. Details of the new protocol and a description of the results of using antibody against the contractile protein, alpha-actinin, are given.
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PMID:Immunoelectron microscopic localization of alpha-actinin on Lowicryl-embedded thin-sectioned tissues. 388 38

Serial sectioning and immuno-ferritin plus peroxidase labeling of ultrathin frozen sections have been used for the simultaneous localization at the electron microscopic level of surface concanavalin A (Con A) receptors and the intracellular contractile protein, myosin, in mouse splenic lymphocytes. An accumulation of intracellular myosin directly underneath the Con A-induced receptor clusters has been observed. This myosin accumulation seems to be restricted to within approximately 1,000-1,500 A from the surface of the cell. This result provides additional support for the possible occurrence of transmembrane interactions between surface receptors and intracellular contractile elements.
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PMID:Simultaneous localization of intracellular myosin and surface concanavalin A receptor clusters using immuno-electron microscopy. 699 97