Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The investigation was conducted to study the role of the determination of tumor markers (CEA, beta 2-microglobulin, IgE and ferritin) in patients with malignant lymphomas. Altogether 66 patients with Hodgkin's disease, 60 with non-Hodgkin's lymphomas and 15 with clinico-hematological remission over one year were investigated, using commercial kits of reagents. An increase in the level of beta 2 was shown to depend on the spreading of a lymphoproliferative process. An elevated level of IgE was noted in Hodgkin's disease whereas a significant rise was unnoticed in non-Hodgkin's lymphomas. An increase in the level of serum ferritin was noted in an advanced and aggressive lymphoproliferative process. The CEA test in malignant lymphomas is not informative.
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PMID:[The importance of the radioimmunological determination of tumor markers in the diagnosis of malignant lymphomas]. 268 5

The nutritional status of an unselected group of 111 children from the village of Bouansa, People's Republic of the Congo, was studied. Comprehensive clinical examinations, anthropometrical measurements and analysis of albumin, prealbumin, ferritin, C-reactive protein (CRP), IgA, IgG, IgM, IgE, IgG- and IgM-circulating immune complexes (CIC) were carried out. The results show, by anthropometrical classification, a high prevalence of moderate malnutrition. Low levels of plasma proteins and high levels of immunoglobulins and CIC were found. No correlation between anthropometrical classification and plasma proteins was established. Children with increased levels of CRP showed low prealbumin values and increased levels of ferritin. Patterns of immunoglobulins and CIC were close to those found in other studies in tropical countries. To evaluate the anthropometrical and biochemical findings it is necessary to take into consideration the apparently healthy appearance of the children, which shows the degree of adaptation to the limited availability of food and the high rate of acute and chronic infections.
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PMID:Investigation of the nutritional state of children in a Congolese village. I. Anthropometrical data, plasma prealbumin, albumin, immunoglobulins, ferritin, C-reactive protein, circulating immune complexes. 323 39

Cytologic examination and determination of tumor markers (PHI, LDH, alpha-1-glycoprotein, alpha-2-HS-glycoprotein, beta 2-microglobulin, ferritin [corrected], sialic acid, IgE, fetoprotein, CEA, beta HCG and beta 1-SP-glycoprotein) were carried out in pleural fluid samples obtained from 70 patients with suspected neoplasia. Tumor markers were also determined in sera. The protein content of all pleural effusions was greater than or equal to 3 g/dl. Patients were grouped according to diagnosis as follows: (a) 42 with neoplastic diseases (7 mesotheliomas and 19 lung, 4 ovarian, 3 breast and 8 miscellaneous cancers), (b) 22 with benign inflammations and (c) 6 with congestive effusions. Of the parameters examined, only CEA and beta-HCG [corrected] gave information that the effusion was probably malignant. Using 6 ng/ml as cut-off for CEA and 10 mIU/ml for beta HCG, the sensitivity was 57.1% and 45.2%, respectively, specificity was 92.8% for both parameters and test efficiency 0.75 and 0.69, respectively. When CEA and beta HCG were considered together sensitivity increased to 73.8% and efficiency to 0.78. CEA and/or beta HCG were positive in the pleural effusions of 19 of the 20 malignant pleural effusions, all with a negative cytologic examination, which subsequently became positive in 8. Because of their high specificity, these two parameters are a useful tool and can be routinely measured to evaluate pleural effusions of dubious origin, even if CEA and beta HCG cannot, on [corrected] their own, define the primary malignancy.
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PMID:Detection of malignant pleural effusions by tumor marker evaluation. 340 38

Blood samples from 40 patients with fissured tongue syndrome (FTS) were examined, and the results were compared with those of 20 healthy control subjects. FTS was diagnosed when a) the patient had a fissured tongue with smooth-surfaced papillae (n = 25) or b) the patient had geographic tongue and some relatives had fissured tongue (n = 15). These tongue forms were verified also histologically. To evaluate the possibility of systemic disorders in patients with FTS we determined the whole blood picture and levels of vitamin B12, serum folate, serum ferritin, and immunoglobulins (IgA, IgG, IgM, IgE). None of the patients with FTS nor any of the controls were found to be anaemic. The mean levels of serum vitamin B12, ferritin and folate were, however, slightly lower in the patient group than in the controls. These findings suggest that anaemia does not play a primary role in the aetiology of fissured tongue syndrome. The most striking haematological findings were the decreased thrombocyte and leucocyte counts in patients with fissured tongue syndrome compared with the control subjects. Furthermore, the lymphocyte count and serum IgG were low in the patient group. When the two patient groups were compared no differences were found. These observations are discussed from the standpoint of deficiency in the immunological defence mechanism of patients with fissured tongue syndrome.
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PMID:Haematological and immunological features of patients with fissured tongue syndrome. 348 1

We have examined human leukocyte preparations for the presence of surface-bound IgE by electron microscopy. Basophil-enriched leukocytes were reacted with burro anti-IgE, a hybrid antibody to burro IgG and ferritin, and ferritin, with or without prior incubation of the cells with an IgE myeloma protein. In the absence of preincubation with IgE small amounts of ferritin were fixed to the surface of basophils but on no other cells. When cells were preincubated with IgE the amount of ferritin fixation on the basophils was markedly increased and a small amount of ferritin was also bound to platelets but again to no other leukocytes. The distribution of ferritin on the basophil surface appeared dependent upon the temperature at which the cells were kept during and after reaction with the various reagents. Basophil sections from cells kept at 0 degrees C had ferritin bound to the surface membrane in patches distributed around the entire circumference. Basophil sections from cells prepared at room temperature had ferritin distributed assymetrically covering a surface membrane segment one-fifth to one-half of the circumference. In control studies in which monomeric IgG was substituted for the IgE and burro anti-IgG was used instead of burro anti-IgE, no cellular fixation of ferritin was observed.
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PMID:Electron microscopic localization of immunoglobulin E on the surface membrane of human basophils. 410 24

The authors studied orthophenylenediamine in order to determine the optimal conditions for its use in "sandwich" type of enzymo-immunology. They studied the kinetics of the reaction and the stopping of the enzymatic reaction in the presence of peroxidase-labeled antibody. Studies of the dissolution of orthophenylenediamine and the stability of the solutions obtained show that a solution of orthophenylenediamine can be stored at ambiant temperature and in darkness for 8 hours. Depending on the degree of sensitivity required, the concentration of orthophenylenediamine can vary between 10 to 30 mmol/l with 10 mmol/l of H2O2. Stopping the reaction with acid greatly increases the values of absorbance by 1.5 to 3 times, depending on the type of acid used. The tests demonstrated that the sensitivity and repeatability are better than can be obtained with 2,2' azino-di(3-ethylbenzthiazoline-6-sulfonic) acid. On the basis of these experiments, orthophenylenediamine was used in the assay of ferritin and total IgE: the increased sensitivity meant that the amount of antigen introduced into the reaction could be reduced to 1/5 of the amount usually required.
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PMID:[Measurement of peroxidase activity with orthophenylenediamine as chromogen in immunoenzyme methods]. 608 17

The events associated with antigen-induced, IgE-mediated degranulation of human basophils from allergic donors were studied ultrastructurally. Partially purified cells were examined prior to addition of antigen and after incubation with antigen or control buffer for 15 seconds to 60 minutes. Reactions were stopped instantaneously by adding fixative directly to the cell suspension. After fixation the cells were exposed to cationized ferritin as a sensitive probe for demonstrating possible continuities between the cytoplasmic granules and the cell surface. Ficoll-Hypaque-isolated basophils were of three types: Type I, cells containing basophil granules with a full complement of particles; type II, cells containing some full granules but also variable numbers of cytoplasmic vacuolar structures having the size and shape of basophilic granules but having reduced or no particle content (partially fixed or empty granules); and type III, basophils containing only empty granules. Following exposure to specific antigen, basophils of all three types underwent degranulation characterized by the fusion of the membranes bounding single basophilic granules with the plasma membrane and leading to extrusion of content. Cells in the process of degranulation (type IV and V basophils) were characterized by communications between individual granules and the cell exterior. Identification of such communications was facilitated by cationized ferritin which entered granules having open communications with the cell surface. Without this marker, the number of such communications would have been seriously underestimated, either because they were extremely narrow, tortuous, or outside the plane of section. The majority of individual basophilic granules fused singly and separately with the plasma membrane, in contrast to guinea pig basophil and rat mast cell degranulation where intercommunicating clusters of granules fused with the plasma membrane at a single point. The particle and membrane contents of extruded granules frequently remained adherent to the surface of type IV and V basophils and were not immediately solubilized. Morphologic evidence of degranulation progressed with time of exposure to antigen, exhibiting kinetics that paralleled histamine release. Cells in control incubations, and rare basophils that had been exposed to antigen E, failed to degranulate. Fully degranulated (type VI) basophils were viable cells that had a markedly irregular surface and were devoid of basophilic granules but retained the minor population of small perinuclear granules.
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PMID:Antigen-induced IgE-mediated degranulation of human basophils. 615 54

In 76 patients with clinically well defined multiple myeloma (median age at diagnosis: 68.5 years) serum-ferritin (SF) and beta 2-microglobulin (beta 2M) were measured by RIA-methods. 70 sex and age-matched healthy individuals served as controls. Both serum-ferritin (median: 343 micrograms/l vs. 193 micrograms/l; p less than 10(-7)) and beta 2M (median: 4.25 mg/l vs. 3.5 mg/l; p less than 0.01) showed a significant increase in myeloma patients compared to controls. Intercorrelation analysis revealed significant correlations between SF and tumour mass, serum-creatinine and beta 2M and between beta 2M and tumour mass, percentage of plasma cell infiltration in bone marrow, agglutinin titer, serum-creatinine, hemoglobin and age of the patients. Both tumour proteins might gain clinical importance particularly in those patients in which precise monitoring of disease is impossible either due to lack of paraprotein production or due to the particular paraprotein type. This seems to account for patients with light chain paraproteins, and furthermore for those patients with biclonal gammopathies or with IgE- and/or IgD-paraproteins.
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PMID:[Serum ferritin and beta-2-microglobulin in multiple myeloma]. 618 72

In 76 patients with clinically well-defined multiple myeloma (median age at diagnosis: 68.5 years), serum ferritin (SF) and beta 2-microglobulin (beta 2M) were measured by RIA methods. Seventy sex- and age-matched healthy individuals served as controls. Both serum ferritin (median: 343 vs 193 micrograms/liter; P less than 10(-7] and beta 2M (median: 4.25 vs 3.5 mg/liter) showed a significant increase (P less than 0.05) in myeloma patients compared to controls. Intercorrelation analysis revealed significant correlations between SF and tumor mass, serum creatinine, and beta 2M, and between beta 2M and tumor mass, percentage of plasma cell infiltration in bone marrow, agglutinine titers, serum creatinine, hemoglobin, and age of the patients. Both tumor proteins might gain clinical importance particularly in those patients in which precise monitoring of disease is impossible either due to lack of paraprotein production or due to the particular paraprotein type. This seems to account for patients with light chain paraproteins, and for those patients with biclonal gammopathies or with IgE and/or IgD paraproteins.
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PMID:Serum ferritin and beta 2-microglobulin in patients with multiple myeloma. 619 21

Improved methods are presented for the detection of antigens and haptens by the use of the passive hemolysis inhibition test. The test is capable of detecting nanogram quantities of proteins (e.g., ferritin, IgE) and haptens (e.g., folinic acid, methotrexate). The method is also useful for studying quantitative and qualitative aspects of antibody-antigen interaction.
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PMID:Immunoassay of antigens and haptens by inhibition of passive immune hemolysis. 696 95


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