Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The characters of liver isoferritin from Wistar rat, normal human and patient with hepatocellular carcinoma (HCC) were studied. The ratio of iron to protein of liver isoferritin from patient with HCC were 0.021, which accounts for about one-twelfth of that of liver isoferritin from normal human. The molecular weights of liver isoferritin from normal human and the patient with HCC were 20.5 kd and 21.5 kd, respectively. The electrophoresis patterns of liver isoferritin from both Wistar rat and patient with HCC showed one band, but two bands from normal human. There was cross reaction in immunity among isoferritins from normal human liver, Wistar rat liver and horse spleen by double immunodiffusion. The PAGE patterns of liver isoferritin from different sources could be clearly differentiated. The data presented may serve as a good reference for further study of the relationship between the structure and function of ferritin and may be used practically in clinical medicine.
Hua Xi Yi Ke Da Xue Xue Bao 1991 Sep
PMID:[A study on characters of liver isoferritin]. 166 43

Thin-layer IEF, an analytical technique of high resolution for protein, was used to identify the liver isoferritin in Wistar rat, normal human and patients with hepatocellular carcinoma (HCC) respectively. The focusing conditions were 2000 V, 25W, 10 degrees C, 2h. The results showed that the IEF bands of the liver isoferritin in Wistar rat, normal human and patients with HCC were 7, 6, 3 and the pI of the liver isoferritin were 5.76-4.75, 5.80-4.85, 4.50-4.43 respectively. The method and data in this paper might be good reference for in-depth study of the structure and function of ferritin and its applications in clinical medicine.
Hua Xi Yi Ke Da Xue Xue Bao 1989 Sep
PMID:[Study on identification of liver isoferritin by isoelectrofocusing in thin-layer polyacrylamide gels]. 256 Apr 59

There was difference between the reactivities of anti-human ferritin monoclonal antibodies, 6D6 and A-hF-C with liver and heart ferritins. 6D6 reacts with liver and heart ferritin with similar intensity, while A-hF-C reacts preferentially with liver ferritin. Two affinity gels were made with 6D6 and A-hF-C respectively, and used to purify ferritins from crude extracts of human liver and heart ferritins. The results have showed that it is a simple and time-saving method for the purification of ferritin with high purity and yield.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 1996
PMID:The Application of Monoclonal Antibodies to the Imunoaffinity Purification of Human Ferritin. 1223 98