Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

There is now substantial evidence indicating that an accumulation of aluminum occurs in grey matter in diseases associated with Alzheimer neurofibrillary degeneration. Four principle sites of aluminum accumulation have been identified in Alzheimer's disease: DNA containing structures of the nucleus, the protein moieties of neurofibrillary tangles, the amyloid cores of senile plaques and cerebral ferritin. Consideration of the extensive information now available on the toxic effects of aluminum in these four loci strengthens the hypothesis that aluminum could be important in the pathogenesis of this neurodegenerative process. The evidence, however, does not support an etiological role for aluminum in Alzheimer's disease. The primary pathogenic events responsible for Alzheimer's disease are presumed to have affected the genetically determined barriers to aluminum resulting in increased amounts of this toxic element to vulnerable target sites.
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PMID:Aluminum and Alzheimer's disease. 355 May 8

DNA single strand breaks (ssb) have been induced in FLC/C cells in culture. They have been visualized in the electron microscope after decoration with biotin-avidin-ferritin complexes and spreading as monomolecular mixed films. This allowed one to determine the average number of decorated ssbs per unit of DNA length applying straight-forward and simple evaluation methods. This method has been used to investigate the DNA alterations by benzo[a]pyrene (B[a]P) on FLC/C culture cells. Thus a B[a]P-DNA damage curve can be constructed as a regression with a correlation coefficient of r = 0.97, while its isomer benzo[e]pyrene (B[e]P) known to have only low mutagenicity under the same experimental conditions is virtually without effect. The method has further informational potential regarding damage distribution and repair of DNA.
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PMID:Electron microscopic visualization of DNA single strand breaks. 367 22

Analysis of myoglobin levels in L6 cells (derived from rat skeletal muscle) by radioimmunoassay shows that myoglobin is not synthesized until after the cells differentiate to form multinucleated myotubes. Thereafter, myoglobin accumulates in a linear fashion for up to 20 days, the longest time for which the cultures may be reliably maintained. Treatment of cultures with hemin increased myoglobin levels in a dose-dependent manner resulting in a 70% increase in myoglobin with 20 microM hemin. Succinyl acetone, a heme synthesis inhibitor, reduced myoglobin levels by 40% while simultaneous treatment with hemin restored myoglobin levels to control values. Treatment of cultures with a variety of Fe(III) chelates known to enhance both iron accumulation and ferritin synthesis in L6 cells had no effect on myoglobin levels. delta-Aminolevulinic acid also had no effect on myoglobin levels. None of the treatments had any effect on either the total soluble protein or DNA content of the cultures, and, therefore, the observed effects appear to be specific for myoglobin. These results suggest that myoglobin is expressed as a function of differentiation and that intracellular heme exerts a regulatory effect on myoglobin levels.
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PMID:Myoglobin expression in L6 muscle cells. Role of differentiation and heme. 372 91

We have developed a vector-primer-cloner-sequencer (VPCS), based on the pUC plasmids, which is easy to prepare. Stable cDNA libraries have been generated from human, rat, and bull testis. The inserts are of various size classes, including high-molecular-weight reverse transcripts which can be easily sequenced. The utility of this VPCS has been demonstrated by isolating a rat ferritin and glyceraldehyde-3-phosphate dehydrogenase-like clone.
DNA 1986 Oct
PMID:A vector-primer-cloner-sequencer plasmid for the construction of cDNA libraries: evidence for a rat glyceraldehyde-3-phosphate dehydrogenase-like mRNA and a ferritin mRNA within testis. 378 Mar 74

The relationship between placental non-haem iron and placental iron transfer has been studied in the guinea pig. From day 25 to day 50, non-haem iron and iron transfer increase. Expressed on placental wet weight or per g of placental DNA, iron transfer and non-haem iron were inversely related; an increase of transfer was accompanied by a decrease of the non-haem iron content. The results are discussed in terms of the hypothesis, that accumulation of non-haem iron in early pregnancy is caused by an imbalance between iron uptake and iron transfer. The steady increase of total non-haem iron till term which has been demonstrated in this study is in contradiction with this hypothesis. The paper describes an alternative hypothesis in which placental non-haem iron, most likely ferritin iron, is assumed to play an active role in the regulation of placental iron transfer.
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PMID:A study of the relationship between placental non-haem iron and iron transfer in the guinea pig: the maturation of the transfer process. 379 26

DNA has been extracted from hamster-human and mouse-human hybrid cell lines, restricted with EcoRI, and hybridised to a probe for the H subunit of human ferritin, pDBR2. Sequences highly homologous to this probe have been found on at least eight human chromosomes: 1, 2, 3, 6p21----6cen, 11, 14, 20, and Xq23-25----Xqter. Only the gene on chromosome 11 appears to be expressed in these hybrids.
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PMID:Genes for the 'H' subunit of human ferritin are present on a number of human chromosomes. 386 45

Six anti-DNA hybridoma autoantibodies were prepared by fusing spleen cells from unimmunized MRL/MpJ/lpr/lpr female mice with BALB/c myeloma cells. The monoclonal antibodies were analyzed by solid-phase ELISA for antigen-binding specificities. Three antibodies (62A2, 85A5, and 43B2) bound ssDNA, TNP-KLH, and recognized an epitope(s) present on insolubilized proteins such as BSA, KLH, ferritin, and insulin. The antibodies bound, with a marked preference, TNP-KLH, either soluble or insoluble. The other three antibodies (35A1, 32C5, and 39D2) bound only ssDNA. However, this binding was inhibited by free flavinic acid. None of the six antibodies bound either cardiolipin or proteoglycans, indicating that they do not recognize the repeating negatively charge units common to cardiolipin, proteoglycans, and DNA. All six monoclonal antibodies were purified by affinity chromatography with TNP-Sepharose. Moreover, both anti-DNA and anti-TNP antibodies from sera of nonautoimmune and autoimmune mice were purified easily on TNP-Sepharose.
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PMID:Murine polyspecific antibodies. I. Monoclonal and serum anti-DNA antibodies cross-reactive with 2,4,6-trinitrophenyl derivatives. 387 76

When biological materials are infiltrated by a water-soluble melamine resin and hardened, they become as hard as glass. This is a prerequisite for extreme thin-sectioning. In this paper, the structural information from unsupported transparent thin sections of beef liver catalase, calf thymus DNA, horse spleen ferritin, insect muscle and rat microtubules is compared to that of normal thin sections. While ferritin molecules (12 nm diameter), microtubule subunits (8 nm long axis) and catalase crystals (8 nm subunit diameter) appear to become mechanically damaged in a 10 nm section (as measured by resectioning), DNA-molecules (3 nm diameter) are satisfactorily preserved during sectioning. Remarkably, for electron phase contrast imaging of unstained cross-sectioned insect muscle, a minimum section thickness of about 30-40 nm is required.
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PMID:Choosing the appropriate section thickness in the melamine embedding technique. 388 13

Biotin-labeled deoxyuridine triphosphate (BiodUTP) has the potential to be a useful affinity probe for studies on DNA repair, if it can be incorporated into DNA repair patches and does not inhibit subsequent steps in the excision repair pathway. We have synthesized BiodUTP by an improved procedure and have used permeable normal human fibroblasts to determine the effect of substituting BiodUTP for thymidine triphosphate on several steps in the excision repair pathway: incision, polymerization, ligation, and nucleosome rearrangement. The results demonstrate that BiodUTP is efficiently incorporated into repair patches and has little or no effect on the repair process. The presence of BiodUMP in ligated repair patches has been used to visualize the repair patches by electron microscopy following incubation with ferritin-labeled avidin. This approach has been used to estimate the maximum size of repair patches induced by ultraviolet radiation.
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PMID:Incorporation of biotin-labeled deoxyuridine triphosphate into DNA during excision repair and electron microscopic visualization of repair patches. 391 82

Highly specific antibodies bound to carcinogen adducts in DNA modified with (+/-)7 beta,8 alpha-dihydroxy-9 alpha, 10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE I) were quantitated by electron microscopy (EM) visualization and these observations were compared with quantitation of adducts by enzyme-linked immunosorbent assay (ELISA). The antiserum, elicited in rabbits following inoculation with BPDE I-modified DNA, has been found to be highly specific in its recognition of BPDE I-deoxyguanosine moieties. Parallel DNA samples prepared for analysis by ELISA and EM quantitation were randomized, encoded, and analyzed to determine extents of carcinogen modification in double-blind studies. After levels of modification were determined by immunoassays, DNA samples were prepared for EM analysis by incubation with amounts of anti-BPdG-DNA serum in excess of that necessary for complete binding of antibody to antigenic sites. At equilibrium, samples were enzymatically digested with papain in order to cleave anti-BPdG-DNA IgG molecules into Fab fragments in situ. Following column exclusion chromatography, BPdG-DNA-Fab complexes were incubated with ferritin-labeled Fab' fragments of goat [anti-rabbit F(ab')2] IgG in amounts in excess of those necessary for complete binding. When DNA samples were modified to between 0 and 40 fmol adduct/micrograms DNA, excellent agreement was obtained between ELISA quantitation and visualization by EM of antibodies bound to adducts.
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PMID:Quantitation by electron microscopy of the binding of highly specific antibodies to benzo[a]pyrene-DNA adducts. 391 1


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