Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Our aim was to study the effects of a chronic exposure to SO2 on the permeability of bronchial epithelium in the rat, in vivo and in vitro, in relation with a marker (ferritin). The anatomical lesions observed in light and electron microscopy considerably increased the passage of ferritin both in vivo and in vitro through the epithelium of trachea and primary bronchi. This increase was still found although more discrete three months after the end of exposure while the histological appearance returned to normal. Those results showed that SO2 induces in upper airways functional abnormalities persisting beyond the necessary lapse of time for the repairing of histological lesions which could therefore intervene in the pathogeny of chronic bronchopathies.
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PMID:[Abnormalities in bronchial permeability after induction of a chronic SO2 bronchopathy in rat (author's transl)]. 55 46

The effects of different SO2 exposures on the uptake and transport of exogenous proteins by the tracheobronchial epithelium were investigated in vivo and in vitro using explanted tissue from rat lungs. By optical and electron microscopy, modifications in ferritin uptake and transport were observed, depending on SO2 exposure and on subsequent structural changes; these changes involved a considerable increase in mucosal permeability, both in vivo and in vitro, affecting the epithelium of the trachea and main bronchi. Such an increase, although reduced, was observed 3 months after SO2 exposure had been discontinued, at a time when the structure appeared normal. These findings suggested that SO2-induced changes in upper respiratory epithelium may be responsible for long-term abnormal permeability and that such changes may contribute to the pathogenesis of chronic bronchopathy.
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PMID:SO2-induced bronchopathy in the rat: abnormal permeability of the bronchial epithelium in vivo and in vitro after anatomic recovery. 740 20

The kinetics of iron release from Azotobacter vinelandii bacterial ferritin (AVBF) was measured by reduction of core iron with S2O4(2-) followed by chelation of Fe2+ with alpha, alpha-bipyridine (bipy). The rate was first order in AVBF and one half order in S2O4(2-), suggesting that SO2- is the active reductant formed by S2O4(2-) = 2SO2-. With zero-order conditions for dithionite and bipy, two consecutive first-order iron release reactions differing by a factor of about 14 were observed with rate constants of 0.0263 and 0.00184 sec-1, respectively, at 25 degrees C and pH 7.0. The faster reaction corresponded to the loss of 1433 iron atoms (91%) and the slower second reaction corresponded to loss of 145 (9%) of the original 1575 iron atoms present. The first reaction increased about twofold with pH variation between 6.5 and 8.0, whereas the second reaction was unchanged in the pH range 5.5-8. Both dramatically increased at pH 5.0. Methyl viologen increased the rate of both reactions about tenfold. The biphasic behavior for iron loss is interpreted as two different populations of iron atoms present in the core of AVBF, the first representing the bulk iron, and the second a group of unique iron atoms released last which may represent iron attached to the interior of the protein shell or iron associated with the heme groups. Kinetic stopped-flow measurements show that the heme is first reduced, followed by reduction of the core iron by reduced heme, suggesting an electron transfer role for heme in AVBF function.
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PMID:A kinetic study of iron release from Azotobacter vinelandii bacterial ferritin. 855 33

Ferrihydrite nanoparticles with nominal sizes of 3 and 6 nm were assembled within ferritin, an iron storage protein. The crystallinity and structure of the nanoparticles (after removal of the protein shell) were evaluated by high-resolution transmission electron microscopy (HRTEM), atomic force microscopy (AFM), and scanning tunneling microscopy (STM). HRTEM showed that amorphous and crystalline nanoparticles were copresent, and the degree of crystallinity improved with increasing size of the particles. The dominant phase of the crystalline nanoparticles was ferrihydrite. Morphology and electronic structure of the nanoparticles were characterized by AFM and STM. Scanning tunneling spectroscopy (STS) measurements suggested that the band gap associated with the 6 nm particles was larger than the band gap associated with the 3 nm particles. Interaction of SO2(g) with the nanoparticles was investigated by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, and results were interpreted with the aid of molecular orbital/density functional theory (MO/DFT) frequency calculations. Reaction of SO2(g) with the nanoparticles resulted primarily in SO(3)2- surface species. The concentration of SO3(2-) appeared to be dependent on the ferrihydrite particle size (or differences in structural properties).
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PMID:Characterization and surface reactivity of ferrihydrite nanoparticles assembled in ferritin. 1704 47