Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The presence of uniform negative charges on the surface of cultured rat glomerular mesangial cells was demonstrated by an ultrastructural marker, cationized ferritin. Interaction between cell surface negative charges and protamine sulfate, stimulated the synthesis of prostaglandins E2, F2 alpha, 6-keto-PGF1 alpha and thromboxane B2 (TXB2) in a dose-dependent manner, reaching a maximum response at protamine concentration of 50 micrograms/ml. The effect of protamine sulfate was reversed by 25 units/ml heparin. The polyanions, L-glutamic and L-aspartic acids, reversed the protamine effect in a dose-dependent manner. Excess substrate, arachidonic acid, masked the protamine sulfate-stimulated PGE2 synthesis by mesangial cells. The effect of protamine sulfate on PGE2 synthesis was rapid, peaked in 5 min and was independent of extracellular Ca2+. A synthetic cation, poly(L-lysine) hydrobromide, exerted a similar effect on cellular PGE2 synthesis in mesangial cells. The effect of poly(L-lysine) was dependent on the molecular mass of the cationic species employed and was maximum at 17 to 90 kDa. The use of large molecular mass polymers of L-lysine (175 and 565 kDa) resulted in a decline in PGE2 synthesis. These observation indicate that, in mesangial cells, changes in cell membrane electrical charge are linked to enhanced biosynthetic activity and eicosanoid synthesis.
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PMID:Effect of polycations on prostaglandin synthesis in cultured glomerular mesangial cells. 240 12

Mineral status of highly trained (HT) women runners (n = 14) and untrained (UT) women (n = 11), as determined from 3-day dietary records, blood, and urine samples, was compared. HT women had significantly higher energy (HT: 2331 +/- 121 vs UT: 1973 +/- 107 kcal/day; p less than 0.05), magnesium (Mg) (HT: 369 +/- 29 vs UT: 262 +/- 18 mg/day, p less than 0.05), and potassium (K) (HT: 3874 +/- 384 vs UT: 2646 +/- 244 mg/day; p less than 0.05) intakes than UT women. However, mean daily intakes of calcium (Ca), iron (Fe), copper (Cu), and sodium (Na) were not different for the two groups. Plasma concentrations and urinary excretion of Ca, Mg, and Cu of HT and UT women did not differ. Plasma Fe concentrations of the two groups were also similar (HT: 21 +/- 2 vs UT: 22 +/- 1 mumol/L), and, although serum ferritin concentrations of HT women were lower than UT women (HT: 18 +/- 4 vs UT: 30 +/- 6 micrograms/L), the difference was not significant. Urinary Na excretion of the two groups did not differ. HT women had a higher K intake than UT women but urinary K excretion of the two groups did not differ. Whether present mineral intakes by HT women are adequate during periods of intensive training remains to be determined.
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PMID:Dietary intakes and biochemical markers of selected minerals: comparison of highly trained runners and untrained women. 240 68

The phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) is known to arrest mitotic activity and induce macrophage differentiation in the U-937 monoblastic cell line. The acute effect of TPA on ultrastructural morphology and endocytic activity of U-937 cells was studied. TPA induced within 15 min a marked enlargement of multivesicular bodies (MVBs), comprising both volume and number of inclusion vesicles (other organelles appeared unchanged). At this stage the MBVs frequently showed tubular cytoplasmic extensions. Inclusion vesicles accumulated in MBVs with prolonged incubation (60 min). Horseradish peroxidase (HRP) and cationized ferritin (CF) added to the medium were routed preferentially to MVBs in TPA-stimulated cells. In contrast to MVBs of unstimulated cells many of the TPA-induced MVBs showed a positive cytochemical reaction to acid phosphatase. The MVBs in cells incubated with ionomycin, a calcium ionophore, did not differ from those of unstimulated cells. Cellular uptake of 125I-HRP was increased five times the control values already after 5 min of TPA stimulation. The uptake increased further with prolonged incubation (60 min), but at a slower rate. Together these indicate a TPA-induced transfer by endocytosis of portions of the plasma membrane to the lysosomal system via MVBs. Consideration of MVBs as part of the receptor-mediated endocytic pathway suggests that this effect of TPA might involve down-regulation of cell-surface receptors. The possibility of MVBs as a proton-sequestrating compartment, responsible for the cytoplasmic alkalinization previously reported for TPA-stimulated U-937 monoblastic cells, is discussed.
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PMID:Increased endocytosis and formation of multivesicular bodies in phorbol ester-stimulated human monoblastic U-937 cells. 253 39

99mTc-HEDP bone scan was carried out on 12 long-time haemodialysed patients, suffering from bone pains. X-ray examinations of the bone and laboratory tests (serum calcium, -phosphor, -alkaline phosphatase, -parathormone, -aluminium, -ferritin) were also performed. The scintigrams were evaluated by two semiquantitative scores. Based on diffuse, increased radiopharmacon uptake of the bones and more than five points in the Fogelman score 5 patients most likely had serious and 3 had moderate hyperparathyroidism. In two patients osteomalacy was presumed based on decreased radiopharmacon uptake of the bones, increased uptake of the soft tissues and zero Fogelman score. Mixed or other bone disease was suggested in two other patients. Good correlation was found between the results of bone scans, the parathormone values and the results of histology obtained after parathyreoidectomy of 4 patients and autopsy of two others. This non-invasive examination (ie. bone scan) is helpful in differential diagnosis of uraemic osteodystrophy and its wide use is proposed in domestic nephrological practice.
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PMID:[Bone scintigraphy in uremic osteodystrophy]. 260 56

We have compared the nutritional status of patients with lepromatous leprosy coinfected with pulmonary tuberculosis (18 cases) with that of lepromatous leprosy (239 cases) and of pulmonary tuberculosis (21 cases) and with that of healthy controls. There was a severe weight loss and reduction of skinfold thickness in the patients with pulmonary tuberculosis as well as in lepromatous patients with associated pulmonary tuberculosis, but not in patients with lepromatous leprosy. Levels in sera of diet-dependent proteins, such as albumin, prealbumin and retinol binding protein, were significantly decreased in all three groups of patients; on the other hand, levels of the diet-independent proteins, such as the immunoglobulins, were raised in all the groups, particularly in the pulmonary tuberculosis patients as compared with healthy controls. Serum transferrin levels were decreased only in the tuberculosis patients with or without lepromatous leprosy, but not in patients with leprosy alone. While haemoglobin levels decreased in all patient groups, serum iron concentrations were reduced most in lepromatous patients concomitantly infected with pulmonary tuberculosis. Serum ferritin levels increased in the sera of pulmonary tuberculosis and lepromatous leprosy patients, but was severely reduced in lepromatous patients with associated pulmonary tuberculosis. Mean serum zinc and calcium levels were decreased in all three groups of patients, while the serum copper concentration was increased in all of them compared with healthy controls. Also, inorganic phosphorus was elevated in tuberculosis and lepromatous patients coinfected with pulmonary tuberculosis, but not in lepromatous patients. Serum calcitonin levels were increased in all patient groups indicating an inverse correlation between serum calcium and calcitonin levels. This is the first comparative report describing the status of macro- and micronutrients in two most important mycobacterial diseases of the third world countries.
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PMID:Undernutrition in lepromatous leprosy. V. Severe nutritional deficit in lepromatous patients co-infected with pulmonary tuberculosis. 270 15

The vertebrate muscle spindle has been observed to be ionically and biochemically isolated from the surrounding muscle fibers by the spindle capsule. We have explored the possibility that the capsular cells are endocytically active and can transport both small molecules and macromolecules into the capsular space. Transcytosis (the endocytic transport of extracellular substances across a cell) through the capsule cell layer was examined with muscle spindles of snake, rat, and cat using fluorescent markers for fluorescence microscopy and horseradish peroxidase (HRP) and ferritin for electron microscopic examination. The fluorescent markers were actively taken up by capsule cells, making it easy to locate the spindle capsular region of spindles among extrafusal fibers by their strong fluorescence. Ferritin and HRP were used to identify the pathway of transcytosis by electron microscopy. These markers were found in endocytic vesicles of capsule cells, in the narrow space between capsule layers and in the capsular space, indicating that the marker was transferred to the capsular space by the pinocytic activity of capsule cells. Scattered cells in the capsule of cat muscle spindles appeared to take up fluorescein isothiocyanate (FITC)-coupled beta-glucuronidase by a receptor-mediated process. The uptake was sensitive to temperature and [Ca2+], and specifically inhibited by yeast mannan. By electron microscopy with dilute HRP (10 micrograms/ml) this specific uptake was by isolated cells in the interlamellar space. The functional significance of the above findings is discussed.
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PMID:Endocytosis and transcytosis by the capsule cell of vertebrate muscle spindles. 280 78

Striopallidodentate calcinosis (Fahr's disease) is characterized clinically by seizures, rigidity, and dementia and pathologically by mineral deposition in the basal ganglia, dentate nucleus, and cerebral cortex. Disorders of iron and calcium-phosphate metabolism are thought to play a role in its pathogenesis. We present the case of a patient with familial striopallidodentate calcinosis who had porphyria cutanea tarda, refractory anemia, and pseudohypoparathyroidism type 2. The serum level of ferritin was markedly increased, serum iron and iron-binding capacity were below normal, and at autopsy she had deposition of iron in liver, spleen, bone marrow, and brain. She showed intermittent mild hypocalcemia, increased serum values of parathyroid hormone, elevated renal tubular reabsorption of phosphate, and low serum levels of 1,25-dihydroxyvitamin D, suggesting blunted renal responsiveness to endogenous parathyroid hormone. Pseudohypoparathyroidism type 2 was confirmed by infusion of synthetic parathyroid hormone, which gave a normal urinary cyclic adenosine monophosphate response, but a blunted phosphaturic response. After splenectomy for hypersplenism and weekly phlebotomies, she showed progressive improvement in function, mental status, weight, and seizure control. The hypothesis advanced is that the underlying pathophysiology of the separate diseases contributed to the formation of the brain stones through mechanisms of defective iron transport and free radical production.
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PMID:Abnormal systemic metabolism of iron, porphyrin, and calcium in Fahr's syndrome. 281 30

In a series of 28 long-term dialysis patients with musculoskeletal complaints, the radiologic findings in six cases resembled those occurring in the arthropathy of idiopathic calcium pyrophosphate dihydrate deposition (CPPD) disease. These findings included osteophytes, subchondral cysts, and cartilage loss in the metacarpophalangeal joints, patellofemoral joints, wrists, and shoulders. Chondrocalcinosis was present in three of the six cases. There were no significant differences in renal function or levels of serum calcium, phosphorus, iron, ferritin, aluminum, or parathormone between these patients and a control group matched for sex and age. Long-term dialysis may be associated with a metabolic arthritis similar to the arthritis which occurs in CPPD deposition disease. The etiology may include deposition of CPPD crystals, hydroxyapatite, or other calcium-containing substances in joints, or it may be related to a number of dialysis-induced metabolic abnormalities.
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PMID:Radiologic features of a pyrophosphate-like arthropathy associated with long-term dialysis. 282 26

Cationized ferritin (200 micrograms ml-1) causes a reduction in both spontaneous and evoked transmitter release at the frog neuromuscular junction. The reduction in EPP amplitude probably reflects reduced Ca2+ -influx since full recovery can be achieved by elevation of [Ca2+]o. The effect on MEPP frequency is independent of [Ca2+]o and may well reflect a direct effect on exocytosis.
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PMID:Inhibition of quantal transmitter release by the polycationic ligand cationized ferritin. 286 66

The regional distributions of iron, copper, zinc, magnesium, and calcium in parkinsonian brains were compared with those of matched controls. In mild Parkinson's disease (PD), there were no significant differences in the content of total iron between the two groups, whereas there was a significant increase in total iron and iron (III) in substantia nigra of severely affected patients. Although marked regional distributions of iron, magnesium, and calcium were present, there were no changes in magnesium, calcium, and copper in various brain areas of PD. The most notable finding was a shift in the iron (II)/iron (III) ratio in favor of iron (III) in substantia nigra and a significant increase in the iron (III)-binding, protein, ferritin. A significantly lower glutathione content was present in pooled samples of putamen, globus pallidus, substantia nigra, nucleus basalis of Meynert, amygdaloid nucleus, and frontal cortex of PD brains with severe damage to substantia nigra, whereas no significant changes were observed in clinicopathologically mild forms of PD. In all these regions, except the amygdaloid nucleus, ascorbic acid was not decreased. Reduced glutathione and the shift of the iron (II)/iron (III) ratio in favor of iron (III) suggest that these changes might contribute to pathophysiological processes underlying PD.
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PMID:Transition metals, ferritin, glutathione, and ascorbic acid in parkinsonian brains. 291 Oct 28


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