Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. From capillary red cell velocity (V)-flux (F) relationships of hamster cremaster muscle a yield velocity (VF = 0) can be derived at which red cell flux is zero. Red cell velocity becomes intermittent and/or red blood cells come to a complete standstill for velocities close to this yield velocity, and, at the same time, capillary tube haematocrit becomes very low. 2. We have tested whether the net negative charge of red blood cells (RBCs) contributes to the magnitude of VF = 0. Velocity-flux relationships were measured for normal cells, normal cells labelled with the fluorescent dye calcein (LRBCs), and red cells treated with hexadimethrine to mask negative charge and labelled with calcein as well (HDM-LRBCs). Measurements were done in a hamster cremaster muscle preparation applying video in vivo microscopy. 3. Hexadimethrine treatment reduced the net negative surface charge of red cells to 20% of control as estimated from transmission electron microscopy using a ferritin tagging technique. The values of VF = 0 found for normal red cells and HDM-LRBCs were 86 +/- 15 and 31 +/- 17 microns s-1, +/- S.E.M., n = 12, respectively, which were significantly different (P < 0.05). For normal cells and cells labelled with calcein only, VF = 0 values were 63 +/- 14 and 65 +/- 13 microns s-1, n = 8, respectively, which were not significantly different. The effect of HDM treatment did not alter filterability of the red cells as estimated from transit times through 5 microns pores. 4. The present findings demonstrate that the net negative charge of RBCs contributes significantly to the yield velocity for red blood cells entering capillaries and flowing through them. HDM treatment reduced the net negative charge of red blood cells and may have caused cells to enter capillaries more easily owing to reduced electrostatic repulsion at the capillary entrance. In addition, HDM treatment may have lowered intracapillary flow resistance by a reduction in electrostatic repulsive forces between red blood cells and negatively charged (macromolecules on) capillary endothelial cells at sites of irregular capillary cross-sectional shape, without significantly affecting the lubricating properties of the capillary endothelial glycocalyx and/or associated plasma macromolecules.
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PMID:Evidence that cell surface charge reduction modifes capillary red cell velocity-flux relationships in hamster cremaster muscle. 858 3

The opisthorchiasis caused by Opisthorchis felineus, the Siberian liver fluke remains a serious public health problem in Russia and Eastern Europe. Proteomic identification of the proteins in the excretory-secretory products (ESPs) released by O. felineus is an important key for the investigation of host-parasite interactions and understanding the mechanisms involved in parasite survival within the host. In the ESP of O. felineus we have identified 37 proteins using high-resolution proteomics approach (LTQ-FT-ICR mass spectrometer). The O. felineus secretes either excretes a complex mixture of proteins including: glycolytic enzymes (enolase, aldolase, fructose-1 ,6-bisphosphatase and other); detoxification proteins (4 isoform of glutathione S-transferases, Cu/Zn superoxide dismutase, thioredoxin peroxidase, thioredoxin); cytoskeletal proteins (beta tubulin and paramyosin); a number of proteases (cathepsin F, B1, leucin aminopeptidase 2); protease inhibitors (putative cys1 protein, leukocyte elastase inhibitor), binding proteins (ferritin, myoglobin, FABP) and other. In the O. felineus ESP we also identified Of-HDM protein belonging to a novel family "helminth defence molecules" (HDMs). The O. felineus proteins identified in this study provide necessary information for the further investigation of molecular mechanisms of opisthorchiasis pathogenesis and some of them would be of interest as potential antigens for vaccine and immunodiagnostics development and as potential new anthelmintic drug targets.
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PMID:[Secretome of the adult liver fluke Opisthorchis felineus]. 2569 23