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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Large quantities of ferritin were found in both seminal plasma and amniotic fluid, but there was a wide spread of results for each of six new non-radioactive monoclonal assays designed to give the same results for ferritin in blood serum. Correlation of values between the various assays was also poor, but after the exclusion of outliers it was possible to calculate approximate ratios for the concentrations. For seminal plasma, the Amersham 'Amerlite' luminescence immunoassay gave the lowest concentrations at 81 +/- 56 ng ml-1 WHO 80/602 (mean +/- SD, n = 43). Relative to this value as 100%, the other assays gave:Flow 'Monoscan' colorimetric IEMA = 161%; Pharmacia-LKB 'DELFIA' fluoroimmunoassay = 174%, Walker 'Synelisa' colorimetric ELISA = 182%; Abbott 'IMx' MEIA = 207%, while the results from the Ramco colorimetric ELISA were much higher and not parallel to the other assays. For amniotic fluid, the Amersham 'Amerlite' LIA gave 127 +/- 95 ng ml-1 (n = 38), taken as 100%, and the ratios for the other assays were:Abbott also approximately 100% but with a wide spread; Walker 43%, Pharmacia-LKB 22% and Ramco 148%. The Flow assay produced 15 zero values from 38 samples, and values were also very low in another 17 samples, so that no comparison with the other assays was possible. An attempt was made to determine the proportion of the different types of isoferritins being estimated. The Pharmacia-LKB assay estimated 90% of the theoretical concentration for pure liver ferritin, 42% of that from the placenta, 26% of that from the heart and 13% of that from the spleen. The other assays gave different proportions.
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PMID:Ferritin: another pregnancy-specific protein in human seminal plasma and amniotic fluid, as estimated by six methods. 209 78

The enzyme linked immunosorbent assay (ELISA) is applied for ferritinemia measurement. Polystyrene beads are used for the adsorption of antibodies and peroxidase as revealing enzyme. The normal values are established with a splenic ferritin solution. The limits of the method are studied and the results are compared with two commercial kits (RIA-Gnost Behring, Ferrizyme Abbott).
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PMID:[Blood ferritin: an immunoenzyme assay technic]. 390 Aug 90

We evaluated four commercial kits for measuring serum ferritin, based on three techniques: immunoradiometric assay, radioimmunoassay, and enzyme immunoassay. The kits evaluated were those manufactured by Abbott Laboratories, Clinical Assays, Corning Medical, and Ramco. Two of the immunoradiometric kits showed satisfactory results with respect to sensitivity and precision; they should be useful in diagnosing individuals with uncomplicated iron-deficiency anemia. One of the immunoradiometric assay kits, however, showed a high-dose "hook effect," beginning at 10 mg of ferritin per liter. We modified this kit to eliminate this effect, at least to ferritin concentrations of 33 mg/L. (We observed a ferritin value as high as 47 mg/L in one patient.) Results with all these kits did not inter-compare well for ferritin concentrations greater than 300 micrograms/L, a finding that casts further doubt on the controversial use of serum ferritin measurement in cases of iron overload.
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PMID:Three commercial methods for serum ferritin compared and the high-dose "hook effect" eliminated. 685 Nov 5

Carcinoembryonic antigen (CEA), neuron-specific enolase (NSE) and ferritin serum levels were assessed before treatment in 109 small cell lung cancer patients. CEA and ferritin serum levels were estimated by immunoenzymatic method: Abbott kits were used. NSE serum level was assessed by radioimmunoassay Pharmacia kits. In 38 patients the disease was localized, in 27 metastases were found in one organ and in 48-in two or more organs. CEA levels above 5 ng/ml were found in 41%, NSE above 12.5 micrograms/l in 86% and ferritin above 250 ng/ml in 41% of patients. The levels of CEA and NSE, but not of ferritin were correlated with the disease extent. The levels of CEA and ferritin, but not of NSE were correlated with performance status of the patients. In the patients with NSE serum levels above 50 micrograms/l and ferritin serum levels above 600 ng/ml the prognosis was significantly worse than in remaining patients.
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PMID:[Level of carcinoembryonic antigen, neuron-specific enolase and ferritin in serum of small cell lung cancer patients: correlation with performance status, disease extent and prognosis]. 811 22

Ferritin was determined in the sera of 631 healthy neonates, infants, children and adolescents (age range 5 days to 18 years), using the IMx from Abbott Laboratories. The applied test was a microparticle enzyme immunoassay (MEIA). The proband collective was divided into 9 age groups, and each group into males and females. In accordance with the recommendations of the International Federation of Clinical Chemistry, the 95% scatter range was taken as the reference range. Only a few reference groups showed a normal Gaussian distribution. In addition to the 50th percentile, the 2.5th and 97.5th percentile were calculated for all reference groups, and the minimal and maximal values were also reported. Significantly different reference ranges were found for males and females in the age group 16-18 years. The U-test of Mann & Whitney was used to test for significant differences between individual reference groups. Groups showing no significant differences were combined, and the corresponding reference ranges for serum ferritin were then calculated.
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PMID:Establishment of reference ranges for ferritin in neonates, infants, children and adolescents. 839 86

Ferritin concentrations in blood are a good indicator of iron stores and can be measured in plasma or serum with commercial kits. We have measured plasma ferritin content in infants ranging from 3 to 15 months of age. During the first three years of a four-year study, plasma samples drawn from these infants were shipped to Abbott Laboratories in Chicago and assayed using the Ferrizyme immunoassay technique (Abbott Laboratories, Chicago, Illinois). For the last year of the study, we analyzed the remaining samples in St. John's using the radioimmunoassay GammaDab125 I Kit (Baxter Travenol Diagnostics, Inc., Cambridge, Massachusetts). Both kit manufacturers report inter- and intra-assay variability of < or = 5%. Results for samples analyzed by the second method were higher than earlier results from infants of the same age with the same intakes of iron; thus, we decided to analyze subsequent samples with both kits.
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PMID:Comparison of two kits for measuring ferritin in blood. 845 43

A commercial ferritin kit (Abbott AxSYM Ferritin) was calibrated using the WHO Human Liver Ferritin International Standard 80/602. The reconstituted WHO freeze-dried standard was diluted to obtain six concentration levels ranging from 10-840 micrograms/l. In the analysis of the data, logarithmic transformation of the results was performed in order to stabilize the variance. The AxSYM kit yielded slightly higher values than the WHO Ferritin Standard (p < 0.05). The relation between the AxSYM kit and the WHO Ferritin Standard (untransformed values) was described by a proportionality: FerritinAxSYM = 1.057 x FerritinWHO. WHO Ferritin Standard values of 12 and 15 micrograms/l (used as cut-off values for absent or small body iron reserves) yielded calculated AxSYM values of 12.7 and 15.9 micrograms/l. A WHO Ferritin Standard value of 30 micrograms/l (used threshold value for the presence of stainable bone marrow haemosiderin iron) yielded a calculated AxSYM value of 31.7 micrograms/l.
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PMID:Calibration of Abbott AxSYM Ferritin kit using the WHO Human Liver Ferritin International Standard 80/602. 929 54

Reference values for two ferritin assays (Tina-quanta Ferritin, Enzymun, both Roche Diagnostics, Mannheim, Germany) were established (136 males and 139 females). To rule out inflammation as well as iron deficiency in the reference population, subjects with the C-reactive protein concentration < 5 mg/l, and zinc protoporphyrin < 40 micromol/mol heme and the soluble transferrin receptor < 3 mg/l were selected. Taking into account latent iron deficiency as well as hereditary hemochromatosis the 5-95 percentile range was as follows: male, 27-365 microg/l; female, 13-148 microg/l for Tina-quanta and 12-151 microg/l for Enzymun. The Tina-quanta Ferritin assay showed a very good correlation (r > or = 0.990) to Enzymun ferritin, Ferritin Abbott (Abbott Diagnostics, Delkenheim, Germany), N Latex Ferritin (Dade Behring, Marburg, Germany) and the Ferritin Chiron (Chiron Diagnostics, Fernwald, Germany). However, the slopes of the standard principal component method analysis were calculated to be between 1.03 (Enzymun) and 1.41 (N Latex Ferritin). For four assays the median recovery of the 3rd International Recombinant Ferritin Standard (NIBSC 94/572) measured by serial dilution was 89-109%. The N Latex Ferritin assay recovered half of the target values. Because of the good correlation with other assays, a matrix effect is likely. The question arises whether the manufacturers' agreement on the recombinant ferritin standard would harmonize ferritin measurement.
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PMID:Reference values for a homogeneous Ferritin assay and traceability to the 3rd International Recombinant Standard for Ferritin (NIBSC code 94/572). 1053 31

The serum ferritin assays, Ferritin RIA Amersham(TM) and Abbott AxSYM(TM) Ferritin were compared in order to translate values from one assay to the other. Serum ferritin was analysed with both assays in 102 samples. Logarithmic transformation of the results was performed in order to stabilize the variance. The relationship between the untransformed values was most exactly expressed by a proportionality: AxSYM Ferritin = 0.873 * RIA Ferritin. Due to this proportionality, the numerical difference between the assays increases with the ferritin concentration, although the percentage difference between the assays remains constant.
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PMID:Erythropoiesis: Short Report: Translation of Analysis Results between Serum Ferritin Assays, Ferritin RIA AmershamTM and Abbott AxSYMTM Ferritin. 1139 62

The routine chemical assays are affected by sample haemolysis, icterus and lipaemia, collectively known as serum indices; however, little attention has been given to the consequences of these conditions on hormonal assays (immunoassays). In this study, we assess the impact of interferences from exogenous serum indices on various endocrine assays performed on the Abbott Architect i2000 system. The pool of 20 serum samples was derived from a hospitalised population. The diluted serum samples were spiked with red cell haemolysate, Intralipid and bilirubin. The interferences were studied at baseline; 12.5%, 25%, 50%, 75% and 100% of 5.0 g/L haemoglobin; 1% of 20% Intralipid; and 0.342 mmol/L of bilirubin according to the EP7-A2 guideline (Interference Testing in Clinical Chemistry; CLSI, USA). Aliquots were analysed in duplicate and/or triplicate for various hormones on the Abbott Architect i2000 immunoassay analyser. Serum ferritin (r2=0.84; P=0.074) and TSH (r2=0.81; P=0.52) levels showed a direct relationship with haemolysis and therefore overestimated because of the effects of haemolysis. The vitamin B12 level progressively decreased as the amount of haemolysis increased (r2=-0.76; P=0.136). There was a significant decrease in progesterone concentration owing to lipaemia (r2=-0.983; P=0.003). For icteric interferences, a strong inverse correlation was observed for folic acid and was shown to be statistically significant (r2=-0.94; P=0.017). Assays for ferritin, TSH, vitamin B12, folic acid and progesterone showed various degrees of interference because of the variability in serum indices.
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PMID:Effects of serum indices interference on hormonal results from the Abbott Architect i2000 immunoassay analyser. 2673 94


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