Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Canine marrow cells were incubated with transferrin-bound (59)Fe, and the partition of cellular iron was studied by chromatographic and gel filtration methods. Splitting-off of iron from the stromal fraction was avoided by lysing the cells in Tris HCl buffer at pH 8.6. Cellular iron was divided into four major compartments: stroma, microsomes, main hemoglobin, and fraction I. The iron in fraction I was found in ferritin, heme proteins, and low molecular weight iron. With incubation times of 3-10 min, (59)Fe appeared promptly in the main hemoglobin. The entry of (59)Fe into ferritin paralleled that of hemoglobin but was smaller in amount. When the marrow cells were incubated with (59)Fe for 15-20 min and reincubated without radioactive iron, movement of (59)Fe into main hemoglobin was observed, and essentially all this iron came from the particulate fraction (stroma, mitochondria, and microsomes). In these chase experiments there was no change in the total quantity of (59)Fe in ferritin. There was no evidence of a significant hemoglobin precursor other than low molecular weight iron. DEPENDING UPON CONCENTRATION, LEAD WAS OBSERVED TO INHIBIT CELLULAR IRON METABOLISM AT SEVERAL POINTS: uptake of iron by the cell, movement of iron from stroma to the soluble intracellular compartment, and synthesis of hemoglobin. The most pronounced inhibitory effect of lead was always on hemoglobin synthesis with an increase in ferritin: hemoglobin ratio. Bipyridine appeared to trap intracellular ferrous iron and to inhibit synthesis of both hemoglobin and ferritin. It was concluded that iron moves from the stroma into the soluble intracellular compartment as low molecular weight iron, probably as a complex of ferrous iron with low molecular weight components of the cytoplasm, that serves as the source of iron for both hemoglobin and ferritin synthesis.
 ...
PMID:Studies on the partition of iron in bone marrow cells. 496 2

Ferritin from horse spleen was found to cause severe chromosome aberrations in cultured Chinese hamster ovary cells. Ferritin at 15 to 170 microgram/ml was clastogenic and at higher doses was cytotoxic. At comparable concentrations of protein or iron, neither apoferritin nor complexed iron was clastogenic. Sulfhydryl compounds glutathione and cysteine reduced the cytotoxic and clastogenic activities of ferritin. Physiological concentrations of glutathione may normally be sufficient to protect cells from damage. The reducing agent ascorbate had little protective effect. Chelating agents varied in their inhibitory activity: ethylenediaminetetraacetic acid (hexadentate) greater than nitrilotriacetic acid (tetradentate) greater than salicylate (bidentate). 2,2'-Bipyridyl enhance the chromosome-damaging action of ferritin while histidine did not markedly alter the frequencies of aberrations. Catalase and superoxide dismutase showed no inhibitory activity. The mechanism of DNA damage may involve reduction of Fe(III) in the ferritin core to Fe(II), followed by reoxidation of Fe(II) with possible formation of free radicals.
 ...
PMID:Chromosome-damaging activity of ferritin and its relation to chelation and reduction of iron. 719 42

Iron is an important element for bacterial viability, however it is not readily available in most environments. We studied the ability of 20 undomesticated food isolates of Bacillus cereus and two reference strains for capacity to use different (complex) iron sources for growth and biofilm formation. Studies were performed in media containing the iron scavenger 2,2-Bipyridine. Transcriptome analysis using B. cereus ATCC 10987 indeed showed upregulation of predicted iron transporters in the presence of 2,2-Bipyridine, confirming that iron was depleted upon its addition. Next, the impact of iron sources on growth performance of the 22 strains was assessed and correlations between growth stimulation and presence of putative iron transporter systems in the genome sequences were analyzed. All 22 strains effectively used Fe citrate and FeCl3 for growth, and possessed genes for biosynthesis of the siderophore bacillibactin, whereas seven strains lacked genes for synthesis of petrobactin. Hemoglobin could be used by all strains with the exception of one strain that lacked functional petrobactin and IlsA systems. Hemin could be used by the majority of the tested strains (19 of 22). Notably, transferrin, ferritin, and lactoferrin were not commonly used by B. cereus for growth, as these iron sources could be used by 6, 3, and 2 strains, respectively. Furthermore, biofilm formation was found to be affected by the type of iron source used, including stimulation of biofilms at liquid-air interphase (FeCl3 and Fe citrate) and formation of submerged type biofilms (hemin and lactoferrin). Our results show strain variability in the genome-encoded repertoire of iron-transporting systems and differences in efficacy to use complex iron sources for growth and biofilm formation. These features may affect B. cereus survival and persistence in specific niches.
...
PMID:Comparative Genomics of Iron-Transporting Systems in Bacillus cereus Strains and Impact of Iron Sources on Growth and Biofilm Formation. 2737 68