Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Isolated pig heart pyruvate dehydrogenase complex (PDC) has been reported to have a molecular mass of 8000 kDa (large PDC) and a diameter of about 45 nm. Studies were carried out to determine the size of PDC in situ. Active enzyme centrifugation showed that extracts of pig heart mitochondria contain, in addition to large (S20,w = 100-200 S) active complexes, catalytically active small PDC (S20,w = 30 S). In addition, small PDC (1000-3000 kDa) could be obtained by gel filtration of mitochondrial extract. If pure large PDC was chromatographed in Triton X-100, then a fraction of it appears in the 1000-3000-kDa range. Isolation of small PDC and rechromatography showed the formation of large PDC. Anti-PDC and ferritin-labeled second antibody were used in an attempt to determine the size of PDC in isolated inner membrane vesicles containing PDC and in permeabilized mitochondria. In both studies no large aggregates of ferritin particles were found which would correspond to the size of large PDC. The conclusion of these experiments is that PDC exists in situ in a smaller form than the isolated pure enzyme.
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PMID:Electron microscopic study on the size of pyruvate dehydrogenase complex in situ. 331 61

Protein nanocompartments (PNCs) are self-assembling biological nanocages that can be harnessed as platforms for a wide range of nanobiotechnology applications. The most widely studied examples of PNCs include virus-like particles, bacterial microcompartments, encapsulin nanocompartments, enzyme-derived nanocages (such as lumazine synthase and the E2 component of the pyruvate dehydrogenase complex), ferritins and ferritin homologues, small heat shock proteins, and vault ribonucleoproteins. Structural PNC shell proteins are stable, biocompatible, and tolerant of both interior and exterior chemical or genetic functionalization for use as vaccines, therapeutic delivery vehicles, medical imaging aids, bioreactors, biological control agents, emulsion stabilizers, or scaffolds for biomimetic materials synthesis. This review provides an overview of the recent biomedical and bioengineering advances achieved with PNCs with a particular focus on recombinant PNC derivatives.
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PMID:The biomedical and bioengineering potential of protein nanocompartments. 3229 94