Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We compared three different methods for radioiodination of human spleen ferritin. Tracers prepared by direct oxidative iodination with use of Chloramine T or 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril (Iodogen) were structurally altered by the labeling procedure, as was made apparent by gel filtration and electrophoresis on polyacrylamide gel. Tracers prepared by conjugation to radioiodinated N-succinimidyl-6-(4-hydroxyphenyl)propionate retained their structural integrity, as assessed by physicochemical methods. Such tracers bound more than 90% in antibody excess, yielded self-displacement curves parallel to unlabeled ferritin standards, and retained their immunoreactivity for nine weeks. By contrast, immunological activity and stability of tracers prepared by the oxidative methods were less satisfactory. Tracers prepared by using lodogen were unsuitable for use in the ferritin radioimmunoassay because of shallow standard-inhibition curves and poor binding in the absence of added standard. Thus, we found the conjugation procedure to be the most suitable of the three methods for preparation of spleen-ferritin tracers.
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PMID:Three different radioiodination methods for human spleen ferritin compared. 47 34

A new experimental procedure for chicken plasma ferritin based on the method of Luxton et al. has been carried out. All biologic reagents used were obtained in our laboratory: Ferritin from chicken liver, antiferritin antiserum from rabbits and anti-rabbit IgG from goat. Chloramine T procedure was applied to label ferritin with 125I. The iodinated protein was removed from free iodide by use of three chromatographic columns of Sephadex G-25, Sephadex G-200 and Sepharose 6B. Separation of the first antigen-antibody complex was carried out by the double antibody method. The assay working range covers from 21.8 up to 175 ng/ml. As ferritin concentration is higher in chicken plasma than in human, various plasma dilutions ranging from 1/16 to 1/64 were used.
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PMID:[Radioimmunoassay of plasma ferritin of Gallus domesticus L]. 733 42