Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The inoculation of the European Alnus glutinosa (L.) Gaertn. host plant by a crushed-nodule inoculum, prepared with the North-American Alnus crispa var. mollis Fern. root nodule, was successful. Fluorescein- and ferritin-labelled antibodies, specific against the A. crispa var. mollis root nodule endophyte (Lalonde et al. 1975), demonstrated the idenity of this endophyte in the resulting nodules. The nodulation process of this abnormal host-endophyte system was studied by light and electron microscopy. An excretion of host blebs containing electron-dense polysaccharide material, resulting in the formation of exo-encapsulation threads containing presumptive endophytic bacterial cells, was associated with deformed root hairs. Originating from an exoencapsulation thread, the endophyte penetrates the root hair cell and then migrates as a hypha toward the cortical cells of the root. Its migration in the cortical cells of the primary nodule results in the induction of a lateral root which develops as the true nodule. The ultrastructure of the A. crispa var. mollis endophyte developing in the primary and true nodule of the abnormal A. glutinosa host was similar to the one induced inside its normal A. crispa var. mollis host. The actinomycetal intruder was a branched and septate hypha able to produce septate vesicles. The endophyte was always encapsulated in an electron-dense polysaccharide material surrounded by a host plasma membrane envelope. However, in this abnormal host-endophyte system, the number of primary nodules formed per root system was drastically reduced, and their appearance was delayed by 1 to 2 weeks. The delayed nodules were effective in fixing nitrogen and able to support satisfactory plant growth in a nitrogen-free medium.
 ...
PMID:Ultrastructural and immunological demonstration of the nodulation of the European Alnus glutinosa (L.) Gaertn. host plant by the North-American Alnus crispa var. mollis Fern. root nodule endophyte. 92 4

Fluorescein isothiocyanate-cationized ferritin (FITC-CF) has hitherto been used mainly to identify structures in living cells by light microscopy, by virtue of its fluorescent properties. We show here that this conjugate can be used, after immediate fixation of the same cell sample and preparation of thin sections, to recognise the same structures, by virtue of the ferritin's electron opacity. The conjugate should thus have a new use as a single-application, dual-purpose probe, e.g. in endocytic studies. The procedure may have advantages over similar dual-purpose probes in not requiring staining or special treatment.
 ...
PMID:Amalgamation of two endocytic probe techniques: fluoresceinated cationized ferritin can show up, sequentially, selected structures, first in living cells and then by electron microscopy. 142 21

The primary decidual zone (PDZ) is a transitory avascular region of transformed fibroblasts surrounding the luminal epithelium at the implantation site. Since this zone may restrict the passage of immunoglobulins, cells, nutrients, and other substances from maternal blood to the epithelium and embryo from Days 6 to 8 of pregnancy, it was of interest to study its permeability to blood-borne tracers. Fluorescein isothiocyanate (FITC)-labeled macromolecules were administered i.v. on Days 6 to 9 of pregnancy. The tracers included dextran (17 kDa), horseradish peroxidase (40 kDa), ovalbumin (45 kDa), dextran (66 kDa), bovine serum albumin (BSA: 66 kDa), dextran (156 kDa), bovine immunoglobulin G (IgG; 160 kDa), and apoferritin (450 kDa). Ten minutes after administration on Days 6 or 7, FITC-labeled tracers of molecular masses of 45 kDa or less were localized in the intercellular spaces of the PDZ and in the blastocyst in small amounts. Tracers with molecular masses of 66 kDa were not detected in these regions up to 1 h after administration but were present in small amounts at 5 h. The 156 kDa and 160 kDa tracers were absent or present only in very small amounts in the PDZ and blastocyst up to 7 h after injection and apoferritin was completely absent at this time. By Day 9 the PDZ had regressed and maternal blood spaces were present adjacent to Reichert's membrane. One hour after administration on Day 9, large quantities of labeled BSA, IgG, and apoferritin appeared in the yolk sac endoderm but not in the underlying embryonic cells. These observations indicate that the PDZ is selectively permeable to blood-borne tracers on Days 6 and 7 of pregnancy, with permeability decreasing with increasing molecular mass. By restricting the passage of high molecular weight substances such as immunoglobulins, microorganisms, and immunocompetent cells, the PDZ may serve a protective function for the embryo, which is no longer protected by the uterine epithelium and has not yet fully developed its own protective layers, especially the yolk sac and Reichert's membrane.
 ...
PMID:Permeability of the primary decidual zone in the rat uterus: studies using fluorescein-labeled proteins and dextrans. 242 Mar 79

Fluorescein-labelled epidermolytic toxin (FTC-toxin) of Staphylococcus aureus and ferritin-toxin conjugate have been prepared and purified. FTC-toxin bound selectively to cryostat and resin-impregnated sections of neonatal mouse skin. Binding was localized at the keratohyalin granules and in the stratum corneum. In an epidermal cell (granular, spinous and basal) preparation, only keratohyalin granules of the granular cells bound FTC-toxin. Ferritin-toxin conjugate bound to skin sections at the same two sites as FTC-toxin and was competitive with the binding of free toxin. Keratohyalin granules in unstained sections had a novel 'patched' appearance under the electron microscope, and the ferritin-toxin conjugate bound preferentially to the electron-lucent areas. In the stratum corneum it was shown by quantitative estimation that the target density decreased as the surface of the tissue was approached.
 ...
PMID:The binding of epidermolytic toxin from Staphylococcus aureus to mouse epidermal tissue. 311 Jan 15

The cell surface of Chlamydia psittaci seems important for establishing infection since (i) UV-treated elementary bodies (EB) attach to and are ingested by L cells and (ii) heat or antibody treatment decreases attachment to L cells and promotes the fusion of chlamydiae-containing phagosomes with lysosomes in macrophages. In the studies reported here, [3H]uridine-labeled UV-treated EB also persisted in mouse resident peritoneal macrophages and L cells, suggesting that phagosome-lysosome fusion is inhibited. We therefore chose to investigate the ingestion and internal fate of isolated purified EB envelopes in both nonprofessional and professional phagocytic cells. EB envelopes are internalized by target host cells as efficiently as are whole EB. Transmission electron microscopy of macrophages whose lysosomes were marked with ferritin revealed the persistence of individual envelopes in phagosomes devoid of ferritin for the 3-h observation period. In contrast, EB envelopes heated to 56 degrees C for 15 min were consistently found in ferritin-labeled phagolysosomes as early as 30 min. As another index of persistence, isolated EB envelopes were radioisotopically labeled with a Bolton-Hunter analog, [3H]N-succinimidyl propionate, and their fate as trichloroacetic acid-precipitable material was followed. A third probe, employed to detect the persistence of non-biodegradable antigen, was indirect immunofluorescence. Fluorescein-positive antigens were brightly visible for 7 days in both macrophages and L cells when they were inoculated with untreated EB or EB maintained in penicillin. But L cells inoculated with EB envelopes or EB treated with UV or chloramphenicol, all of which prevent the conversion of infectious EB into the metabolically active reticulate bodies, displayed reduced internal fluorescence by 2 days and the appearance of fluorescent material on the cell surface. This release of EB envelope material occurred in the absence of phagolysosome fusion. The data add credence to the belief that the spontaneous breakdown or autolytic enzyme release of EB envelope components must occur preparatory to the conversion of EB to reticulate bodies.
 ...
PMID:Chlamydia psittaci elementary body envelopes: ingestion and inhibition of phagolysosome fusion. 684 Aug 60

The arrangement of carbohydrate molecules on surfaces of fungal cells may play an important role in nonself recognition of these microorganisms by potential invertebrate hosts. Changes in the ability of various galactose and mannose-specific lectins to bind to surface components on cell walls of the insect pathogen Paecilomyces farinosus were therefore examined during growth and differentiation of the fungus. Fluorescein isothiocyanate conjugates of concanavalin A (Con A, specific for alpha-D-mannose) and peanut agglutinin (PNA, beta-D-galactose) bound inconsistently to blastospores and weakly to mycelia except at apical regions where strong fluorescence was observed. Labeling patterns were similar on cells tested with a galactose-specific lectin purified from Spodoptera exigua (beet armyworm) hemolymph, but Bandeiraea simplicifolia lectin (BS-I alpha-D-galactose) bound only to mycelia. Electron microscopy using ferritin and gold probes showed that the galactomannans are located in a loosely bound coating on the cell wall surface. Variations in lectin binding patterns are apparently due to absence (e.g., by shedding) of the coat or to rearrangement of carbohydrate components in the coat. Staining of Western blots of dithiothreitol (DTT) cell wall extracts further indicated that the BS-I-binding entity is a unique component of the mycelial surface since, as in the fluorescence studies, blastospore preparations were not labeled. Staining of blastospore blots with other galactose-specific probes (e.g., PNA) was comparable to staining of mycelial blots.(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:Variations in the ability of galactose and mannose-specific lectins to bind to cell wall surfaces during growth of the insect pathogenic fungus Paecilomyces farinosus. 833 Jun 30

A 56-year-old Japanese man was diagnosed as having hereditary ceruloplasmin deficiency. His ceruloplasmin concentration was below the lower limit of detection. Serum copper and iron concentrations were below normal, but the ferritin concentration was highly elevated. An ophthalmoscopic examination showed retinal degeneration with yellowish discoloration of the fundus in both eyes. Fluorescein angiography demonstrated a dark choroid in the posterior pole. Geographic areas of window defects were seen in the midperipheral fundus. The retinal degeneration in this patient was thought to be caused by the cellular iron deposition that occurred as a result of ceruloplasmin deficiency.
 ...
PMID:Retinal degeneration in hereditary ceruloplasmin deficiency. 943 77