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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Previous investigations have shown that alveolar macrophages from cigarette smokers contain more iron than do macrophages obtained from nonsmokers. To localize intracellular iron and to help assess its potential for participation in the production of hydroxyl radicals, macrophages were fractionated and the ferritin and iron contents were measured in various cell fractions. Alveolar macrophages from seven smokers and six nonsmokers were lysed by nitrogen cavitation and centrifuged, first at 500 g and then at 11,000 g. Measured by radio-immunoassay, the total cellular ferritin was 133.8 +/- 33.2 ng and 782.0 +/- 177.8 ng per 1 x 10(6) macrophages (mean +/- SEM, p less than 0.01) obtained from nonsmokers and smokers, respectively. The total cellular iron contents were 7.5 +/- 0.6 nmol and 27.6 +/- 4.8 nmol per 1 x 10(6) macrophages (p less than 0.02) obtained from nonsmokers and smokers, respectively. The accumulation of iron by smokers' alveolar macrophages correlated with the number of cigarettes that had been smoked. Forty-two percent of the iron but only 9% of the ferritin was contained in the pellet obtained from centrifugation at 500 g. The pellet from the second centrifugation contained approximately 33% of the iron and 5% of the ferritin. The supernatant resulting from the second centrifugation contained 25% of the iron and 85% of the ferritin. Cigarette smoking did not appear to alter the intracellular distribution of either iron or ferritin. The ferritin content of bronchoalveolar lavage fluid was 0.10 +/- 0.04 micrograms/mg and 0.90 +/- 0.18 micrograms/mg albumin for nonsmokers and smokers, respectively (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Iron and ferritin contents and distribution in human alveolar macrophages. 337 7

The development of the cerebral microvasculature of the rat was studied during three successive postnatal periods, namely: 1) neonatal period, i.e., 1 to 9 days after birth (capillary sprouting period); 2) myelinization period, i.e., 10 to 20 days; and 3) young adult period, i.e., 2 to 3 months. The survey covered structural aspects and distribution of binding sites for anionic or cationic probes and for albumin-gold complexes on the luminal surface of the microvascular endothelium. The salient results are: a) an extensive development of the endoplasmic reticulum of endothelial cells during the first period (presumably in relation with the production of basement membrane components); b) the high surface density of coated pits and coated vesicles that peaks during the myelinization period; c) the paucity of plasmalemmal vesicle and their differential distribution (their volume density is higher in the endothelium of arterioles than in that of capillaries and venules); d) the existence of an extensive smooth surface tubular system in the cytoplasm of endothelial cells, whose structural connections and functional significance remains to be established; and e) the presence of pericytes with elaborate interactions with endothelia in the early developmental periods. Labeling by perfused tracers indicates an uneven patchy distribution of binding sites for cationic ferritin (generally limited to the plasmalemma proper) and a more even distribution of binding sites for cationic and anionic hemeundecapeptides. Binding patterns did not change during the developmental periods studied. No binding sites were detected for albumin-gold complexes.
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PMID:The cerebral microvasculature of the rat: structure and luminal surface properties during early development. 339 64

Pediatricians often have to assess the nutritional status of their patients from unprecise anamnestic information, unreliable clinical signs or biological data difficult to interpret. None of these parameters can be used alone to establish the nutritional status of a sick child. The validity of a simple and rapid nutritional score was tested. The score consisted of 3 anthropometric parameters (weight, height, triceps skinfold) and 4 biological analyses frequently used in pediatric practice (albumin, transferrin, alkaline phosphatase and ferritin serum determinations). The usefulness of the score was established through the important nutritional deficiencies observed in 80 children admitted for "homing diarrhea" requiring early nutritional support.
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PMID:[Validation of fast nutritional scoring. Application to 80 cases of "homing" diarrhea]. 341 13

Infusion into rats of the polycation hexadimethrine (HDM) leads to onset of massive proteinuria, which recovers when the infusion is stopped. Several lines of evidence indicate that the proteinuria results from binding of HDM to polyanions of the glomerular filter, with neutralization of shielding of their charges. To determine the mechanism of this proteinuria, we measured the glomerular sieving coefficients of anionic and neutral forms of albumin and IgG in control and proteinuric rats. Surprisingly, these studies revealed a marked defect in size dependence, but not in charge dependence, of glomerular permselectivity in hexadimethrine-treated animals, indicating that binding of HDM induces a structural change in the glomerular filter. In vitro studies of binding of tritiated HDM and cationized ferritin to glomerular basement membrane (GBM) indicate that the binding is not dependent on proteoglycans such as heparan sulfate, nor on sialoproteins such as podocalyxin, but is dependent on charged carboxyl groups of GBM.
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PMID:Charged compounds of the glomerular filter and their role in normal and disordered permselectivity. 343 10

We assessed the nutritional status of 302 menstruating women living in three urban, semi-rural and rural areas of eastern Algeria. The anthropometric data and the biochemical measurements (serum levels of total proteins, albumin, transferrin and prealbumin) have shown the absence of protein malnutrition and the evidence of problems of overweight, whatever the criterion used (body mass index or relative weight). There were no differences according to the residence. Anemia (defined by WHO references) was observed in 28% of urban women, 19% of semi-rural women and in 32% of rural women. Iron deficiency (defined by the association of serum ferritin level of 12 micrograms/l or less and transferrin saturation less than 15%) was observed in 29, 27 and 22% of the cases, respectively. Folate deficiency (defined by concentration of red blood cell folates of less than 100 micrograms/l) was observed in 48, 45 and 22% of cases, respectively. Finally, 81% of anemia were associated with biochemical evidence of iron and/or folate deficiency.
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PMID:[Assessment of the nutritional status of Algerian women in the reproductive age living in an urban, rural and semi-rural area]. 349 8

The group B Streptococcus is one of the most virulent organisms causing perinatal infection. Human amniotic fluid from the second and third trimesters was pooled and analyzed for electrolytes, protein, albumin, zinc, inorganic phosphorus, ferritin, lysozyme, and immunoglobulins. We inoculated replicates of specimens with known virulent strains of group B streptococci (893, 891, and 878) and Escherichia coli (C5) with Todd-Hewitt broth and normal saline solution used as controls. Group B streptococci strains 893 and 891 proliferated rapidly at rates similar to their rates in Todd-Hewitt Broth. Strain 878 grew at a rate slower than that of strains 893 and 891. The amniotic fluid specimens were similar with respect to factors reported as inhibitory to bacterial proliferation. Second- and third-trimester amniotic fluid supports the growth of group B streptococci as well as a culture medium optimized for bacterial growth. Strain-specific variance in group B streptococci growth rates in amniotic fluid may have clinical significance for those at risk for group B streptococci infection.
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PMID:Proliferation of group B streptococci in human amniotic fluid in vitro. 354 26

In order to assess the possible effects of insulin on serum concentrations of trace metals (iron, copper, zinc) and trace metal binding proteins (ferritin, transferrin, coeruloplasmin), five normal females were studied with the hyperinsulinaemic-euglycaemic clamp technique. A 0.1 U/kg insulin bolus was administered, followed by an insulin infusion at a rate of 10 mU/kg/min for 12-16 h. Insulin levels of 1500-2000 microU/ml (9.21-12.28 nmol/l) were attained. When iron levels in serum were assayed colorimetrically, there appeared to be a progressive rise in the mean concentration during the course of the insulin infusion. Direct analysis of serum samples by atomic absorption spectrophotometry also showed that the level of non-haeme iron increased 3-fold in the serum of the subject with the lowest concentration of this metal at the start of the study. In contrast with the results for serum iron, the levels of ferritin, total iron binding capacity (transferrin), zinc, copper and coeruloplasmin were not altered in any subject during the insulin infusion or at 24 h following discontinuation of the infusion. Within 4 h of institution of the hyperinsulinaemic clamp significant reductions in serum levels of potassium, phosphorus, cholesterol, total protein and albumin were noted. As the insulin infusion progressed, the urea nitrogen, uric acid and bicarbonate levels fell as well. These observations suggest that supraphysiologic hyperinsulinaemia of 12-16 h duration may alter serum levels of iron, but not serum levels of zinc, copper or trace metal binding proteins in some individuals.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Effects of extreme hyperinsulinaemia on serum levels of trace metals, trace metal binding proteins, and electrolytes in normal females. 354 94

Anionic sites have been demonstrated in the basement membranes of peritubular capillaries. The anionic barrier function of peritubular capillary wall has been ascribed to these sites. Fenestrated capillaries in other organs have anionic sites in the endothelial cell glycocalyx and at the luminal surface of the fenestral diaphragms. The purpose of this study was to map anionic sites at the luminal surface of peritubular capillaries and to assess whether a concentration gradient for albumin exists across the endothelium. Partial chemical characterization of these anionic sites was done by in vivo enzymatic degradation. The difference in distribution of albumin following enzyme digestion was also studied. The binding of cationized ferritin to the luminal surface indicated that the rat peritubular capillaries have anionic sites along the entire luminal surface of the endothelial cell, including the fenestral diaphragms. Partial biochemical characterization of these sites shows that the sites in the glycocalyx are mainly from neuraminic acid, while the fenestral diaphragms have mainly heparan sulfate proteoglycans. Intravascular albumin extended to the endothelial luminal plasmalemma and to the luminal surface of fenestral diaphragms. Digestion with heparitinase was associated with the leakage of albumin outside the capillary wall. These findings suggest that the anionic surface of fenestrae constitutes a charge barrier of the peritubular capillaries.
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PMID:The anionic sites at luminal surface of peritubular capillaries in rats. 356 Jun 45

The uptake of formalin-denaturated homologous albumin (FDA) by rat liver sinus-lining cells was studied using ultrastructural, cytochemical, and immunocytochemical techniques. Three minutes after intravenous injection of: 1) FDA, 2) peroxidase-coupled FDA (HRP-FDA), 3) ferritin-labeled FDA (FE-FDA), 4) colloidal gold-labeled FDA (CG-FDA), or 5) 0.85% NaCl, livers were fixed by perfusion with two different fixatives. Liver sections were processed to cytochemical, immunocytochemical, or immunoelectron microscopical procedures. By light microscopic immunocytochemistry (groups 1 and 5), discrete granular staining was seen in endothelial cells, Kupffer cells, and parenchymal cells. Whereas the staining in endothelial cells and Kupffer cells was much weaker in group 5 than in group 1, no difference was noted in parenchymal cells between the two groups. By immunoelectron microscopy, albumin was localized in coated pits, coated vesicles, endosomes, and phagosomes of endothelial cells and Kupffer cells. In parenchymal cells, however, albumin was confined exclusively to the secretory apparatus. In group 2 (HRP-FDA) the reaction product was localized only in coated pits, vesicles, and endosomes of endothelial cells and Kupffer cells, but not in parenchymal cells. Similarly, in animals injected with FE-FDA and CG-FDA, the ferritin and gold particles were found exclusively in the same intracellular compartments of the sinus-lining cells. The results strongly suggest that FDA is internalized by endothelial cells and Kupffer cells through a receptor-mediated endocytosis.
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PMID:Uptake of formalin-denaturated albumin by the sinus-lining cells of rat liver: an immunocytochemical and cytochemical study. 362 63

Ferritin which had been radioiodinated using chloramine T exhibited marked instability on storage at 4 degrees C. Both [125I]human liver and heart ferritins showed a similar rate of decline in immunoreactivity (t 1/2 = 20-23 days) indicating that deterioration with storage was not a function of isoferritin composition. The decrease in radioactivity associated with ferritin was due not only to loss of 125I from the molecule but also to protein degradation as shown by enzyme-linked immunoassay and gel filtration. The degradation products had an Mr of at least 69,000 although low Mr material could be identified by gel filtration when a marked reduction in immunoreactivity had occurred. Ferritin instability was much more pronounced than when other proteins such as immunoglobulin and albumin were radioiodinated with chloramine T. These observations indicate that when performing in vivo and in vitro studies with labeled ferritin, degradation of the protein during storage should be carefully monitored and the protein repurified before use.
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PMID:Stability of radioiodinated ferritin. 367 24


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