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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This study was designed to investigate the usefulness of serum ferritin determinations for the diagnosis of cervical squamous cell carcinoma. The origin of ferritin in the circulation of these patients was also studied by an in vitro incubation system. Ferritin levels were determined by a radioimmunoassay kit (SPAC kit, Daiichi Radioisotope Lab.). Pretreatment serum ferritin levels were significantly higher (p less than 0.05) in patients with cervical squamous cell carcinoma, ovarian carcinoma, hepatitis and anemia than in normal women. All cases with endometrian cancer showed normal ferritin levels. Among patients with cervical squamous cell carcinoma, stage IV and recurrence groups showed higher ferritin levels than other stages. In vitro incubation studies revealed that squamous cell carcinoma could release significantly larger amount of ferritin than normal squamous epithelium. In addition, circulating and tissue ferritin of squamous cell carcinoma had the same immunological behavior in a ferritin radioimmunoassay, and also showed the identical localization on isoelectrofocusing gels. These results indicated that (1) circulating ferritin in patients with squamous cell carcinoma would, at least in part, be derived from the tumor tissue, and (2) serum ferritin determinations would be useful for the management of patients with cervical squamous cell carcinoma.
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PMID:[Ferritin levels in patients with cervical squamous cell carcinoma (author's transl)]. 723 35

Eleven commercial kits for serum ferritin have been compared. Seven kits used RIA and four IRMA methodology. Seven kits used human liver ferritin, two human spleen ferritin and two undefined human ferritin as standard material. Incubation times varied between 30 minutes and 36 hours. Only four kits gave normal ranges determined with the kit in question. All values were compared with the kit in routine use. Although correlation of results was good in 10 cases, (r = 0.903-0.993) the numerical values varied greatly, (slope of the regression line b = 0.437-1.94). These values were obtained from at least 30 sera for each kit. Seven kits gave significantly different results from the routine kit (p less than 0.05 -- Wilcoxon matched pairs test -- two tail value). Inter and Intra-assay coefficients of variation were under 12.5% in all cases within the range of interest. Four kits showed a pronounced non-linear correlation with the routine kit, and one of the IRMA kits exhibited a high-dose hook effect within the standard curve. The other three IRMA kits showed no such effect up to 2000 micrograms . liter-1 (under assay conditions).
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PMID:A comparison of eleven commercial kits for the determination of serum ferritin levels. 728 74

1. Serum samples were collected from ten patients hospitalized for acute infections and from a control group of seven normal subjects. Tissue ferritin was obtained by purification of ferritin from normal human liver and from the ferritin standard of a commercially available assay kit. 2. The serum and tissue samples were incubated with concanavalin A--Sepharose, which has the ability to bind normal serum ferritin. 3. Concanavalin A, a plant lectin which binds to glucose, can be coupled to Sepharose particles and by incubation and centrifugation ferritin in normal serum can be absorbed to about 70%. The serum and tissue samples were incubated with concanavalin A--Sepharose and the ferritin content was measured before and after. 4. It was found that ferritin in the serum of patients with acute infections was absorbed to the same extent as in normal serum (about 80%), irrespective of the initial value. Only about 20% of the tissue ferritin was absorbed. 5. It is concluded that the ferritin in serum during infection is of the same glucosylated type as the ferritin normally present in serum, whereas intracellular ferritin is not glycosylated. This indicates that the elevation of serum ferritin during infection is caused by a release along the normal pathways, i.e. an augmented synthesis, not by leakage from damaged cells.
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PMID:The source of serum ferritin during infection. Studies with concanavalin A--Sepharose absorption. 742 3

A commercial ferritin kit (Amersham Ferritin RIA (A)) was calibrated using the WHO human liver ferritin international standard 80/602 (W). The reconstituted WHO freeze-dried standard was diluted to obtain five concentration levels ranging from 10-800 micrograms/l. Logarithmic transformation of the values was performed in order to stabilize the variance, yielding the regression equation: logA = 0.0235 + 1.0022 logW. The slope of the regression line (being very close to, and not significantly different from, one) was set to one, and the relation between the untransformed values then became a proportionality: A = 1.067 x W. A WHO standard ferritin value of 15 micrograms/l (often used as cut-off value for absent iron reserves) and of 30 mu/l (often used as threshold value for the presence of stainable marrow haemosiderin iron) yielded calculated Amersham Ferritin RIA values of 16.0 micrograms/l and 32.0 micrograms/l.
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PMID:Calibration of the Amersham Ferritin RIA kit using the WHO human liver ferritin international standard 80/602. 816 93

In a group of 74 patients with multiple myeloma the authors revealed elevated values of serum thymidine kinase (REA kit ADICO Praha, range of normal values 0-5 U/l) in 40% of the patients-incl. a group of 22 subjects examined at the time of diagnosis of the disease in 50%, and a group of 52 subjects examined in different stages of the disease in 36% of the patients. If the upper range of S-TK 10 U/l was used, the ratio of patients with a raised value declined to 15%, in selected groups to 18 and 14% resp. The authors found a satisfactory correlation of serum thymidine values and values of S-beta-microglobulin, S-albumin, with the percentage ratio of plasmocytes in bone marrow and a less significant correlation was found with the red cell sedimentation rate (in IgG and IgA type) to the index of paraprotein and the serum interleukin-6 level. The authors did not reveal significant differences of serum thymidine kinase levels with regard to age, sex and immunochemical type of M-protein and type of light chains. The authors did not reveal any correlation of thymidine kinase serum levels and haemoglobin values, S-ferritin levels, the beta 2-microglobulin index and the synthetic score of plasma cells. It was found that examination of S-thymidine kinase extends in a useful way the existing spectrum of laboratory tests which help to elucidate the individual character of multiple myeloma.
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PMID:[Serum thymidine kinase in multiple myeloma: I. Relation to selected laboratory indicators in the disease]. 818 66

Two commercial ferritin kits, Phadebas Ferritin PRIST (kit B) and Ferritin RIA Amersham (kit A) were compared in order to "translate" ferritin values from one kit to the other. Ferritin levels in 222 sera were determined with both kits in the concentration range 5-838 micrograms/l. Regression analysis disclosed a parabolic regression between the logarithmically transformed results obtained with the two kits. Measured kit B values of 10, 12, 15, 30 and 300 micrograms/l corresponded to calculated kit A values of 15, 17, 20, 34 and 321 micrograms/l, respectively. In kit A, storage of sera for two years at -25 degrees C in combination with freeze-thawing three times produced a minor fall (p < 0.0001) in ferritin levels with a median percent decline of 16%.
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PMID:Statistical pitfalls in the comparison between two commercial serum ferritin kits, Pharmacia and Amersham. 822 74

With a newly developed short term enzyme linked immunosorbent assay kit (TOYOBO Co.), in which 2 kinds of anti-EPO monoclonal antibodies were used, we assayed EPO concentration in sera from patients with renal failure and hematological disorders. In this report, the EPO data were analysed in relation to serum iron concentrations, with ferritin and UIBC. In the patients with renal failure, there was no significant correlation between EPO concentration and serum iron, ferritin, nor UIBC concentration. On the other hand, in the patients with hematological disorders, there were two types. One was in patients with iron deficiency anemia, whose serum EPO was negatively correlated to serum iron (r = -0.64) and ferritin (r = -0.59), but positively related to UIBC (r = 0.27). The another was the pattern in patients with aplastic anemia, leukemia and MDS, whose serum EPO positively correlated to iron and ferritin but negatively correlated to UIBC. In the patients with aplastic anemia serum EPO had good correlation to serum iron (r = 0.62), ferritin (r = 0.60) and UIBC (r = -0.46). The relationship of EPO to iron in the patients with leukemia (r = 0.54), and EPO to ferritin in the patients with MDS (r = 0.42) show significantly positive correlation coefficient.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Assay of erythropoietin in serum with short term enzyme linked immunosorbent assay method--the clinical significance: Part 2--:Relation to serum iron, UIBC and ferritin in renal failure and hematological disorders]. 835 May 9

Ferritin concentrations in blood are a good indicator of iron stores and can be measured in plasma or serum with commercial kits. We have measured plasma ferritin content in infants ranging from 3 to 15 months of age. During the first three years of a four-year study, plasma samples drawn from these infants were shipped to Abbott Laboratories in Chicago and assayed using the Ferrizyme immunoassay technique (Abbott Laboratories, Chicago, Illinois). For the last year of the study, we analyzed the remaining samples in St. John's using the radioimmunoassay GammaDab125 I Kit (Baxter Travenol Diagnostics, Inc., Cambridge, Massachusetts). Both kit manufacturers report inter- and intra-assay variability of < or = 5%. Results for samples analyzed by the second method were higher than earlier results from infants of the same age with the same intakes of iron; thus, we decided to analyze subsequent samples with both kits.
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PMID:Comparison of two kits for measuring ferritin in blood. 845 43

Serum ferritin was measured by six enzyme immunoassays in specimens from patients with digestive cancers (n = 30) and hematologic malignancies (n = 33). Most mean comparisons show significant differences in both groups of patients. In digestive cancers correlations between any two methods are very satisfactory (r > 0.99) but a proportional bias is often observed. In hematologic malignancies, correlations are bad (r < 0.80 in 8 out of 15 correlations) because of many discrepant values. Isoelectric focusing separation of isoferritins was performed in most specimens and the pattern of each serum was compared to the between kit CV. We conclude that an 'acid' spectrotype increases between-kit analytical variability. We try to explain the results taking into account the nature of the immunological systems and the cross-reactions with tissular isoferritins. In conclusion, our results indicate that large differences may be observed in sera from hematologic malignancies (leukemias, lymphomas ... ) We recommend that monitoring be achieved by the same method of measurement.
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PMID:Comparison of six serum ferritin immunoassays and isoferritin spectrotypes in malignancies. 859 13

The aim of this study was the evaluation of an immunoturbidimetric measurement procedure for the concentration of ferritin in serum, based on the new Tina-quant Ferritin reagents kit (Boehringer Mannheim) in which the antibody-coated latex particles have been modified with regard to the previous Tina-quant Ferritin reagents kit. The evaluation was carried out using the BM/Hitachi 917 Analyzer. The evaluation included the within-run and between-day imprecisions estimation, the comparison of measurement procedures and the assessment of the measuring range. An additional study of between-day imprecision was done using 4 microl and 20 microl of sample. Detection limit was studied using BM/Hitachi 917 (4 microl and 20 microl of sample), ES 300 and Cobas-Core analyzers. The coefficients of variation obtained in the between-day imprecision study are lower when 20 microl of sample is used instead of 4 microl. In the measurement procedure using 20 microl, the coefficient of variation observed at physiological concentrations is 2.4%. The detection limit with 20 microl of sample is lower than with 4 microl. Therefore, in ferropenic anaemia studies it is convenient to use the measurement procedure with 20 microl sample volumes, since it possesses the best metrological characteristics for this purpose.
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PMID:Evaluation of a new measurement procedure for the concentration of ferritin in serum. 905 55


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