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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We describe a one step "sandwich"-type immunoenzymoassay for ferritin in human serum. The solid-phase consists of glutaraldehyde-treated polypropylene tubes coated with rabbit antibody to human ferritin. Liver ferritin is the standard. Peroxidase-conjugated antiserum to ferritin and a sensitive chromogen, o-phenylenediamine, are used. The assay requires 90 min. The standard curve is linear up to 400 micrograms of ferritin per liter of serum. Within- and between-run CVs are less than 6% for low, high, and medium concentrations and are about 13.0% at the decision level for iron deficiency. Results by a two-step "sandwich" procedure (New England Immunology Associates kit) correlated well, r = 0.98. We assessed four liver ferritin standards from different manufacturers with the described method. The mean absorbance for the 40 micrograms/L ferritin standard was 1.5 for that from Diagnostics Biochem and National Institute for Biological Standards and Controls, 1.0 for that from Dako, and 0.4 for that from Sigma. Consequently, to standardize results, all liver ferritin standards should be calibrated vs the National Institute for Biological Standards and Controls reference standard.
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PMID:Determination of serum ferritin by a one-step immunoenzymoassay, and comparison of four liver-ferritin standards. 388 84

A biotin-avidin enzyme immunoassay (EIA) for measurement of ferritin in human serum is described. This procedure involves the use of specific antibody immobilized on beads, biotin-labeled specific antibody and enzyme-labeled avidin. Reproducible results were achieved within 2 h for ferritin in serum in the range of 5-500 ng/ml. Ferritin levels as low as 0.1 ng/ml can be measured. The biotin-avidin EIA, a radioimmunoassay kit, and an EIA kit were used to determine serum ferritin levels in healthy adults. Good agreement was found between the biotin-avidin EIA and the other methods.
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PMID:A biotin-avidin enzyme immunoassay for the quantitation of serum ferritin. 388 4

The clinical significance of serum ferritin as a serological marker of hepatocellular carcinoma (HCC) was studied. Fasting serum ferritin levels were measured in 343 patients with diseases of the liver, using a radioimmunoassay ferritin kit. Elevated ferritin levels were obtained in various liver diseases but hyperferritinemia could be more clearly interpreted by classifying ferritin levels according to serum iron or transaminase values. Significantly higher values were obtained in HCC than liver cirrhosis. Sensitivity for diagnosis of HCC increased by serial and simultaneous determinations of ferritin and alpha-fetoprotein because high ferritin levels were observed more often in low alpha-fetoprotein-producing HCC and also in HBsAg negative, alcohol related, small-sized HCC. Therefore, simultaneous determination of alpha-fetoprotein and ferritin seems to be useful for detection of HCC in high risk patients such as those with liver cirrhosis.
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PMID:Clinical significance of serum ferritin determination for hepatocellular carcinoma. 620 85

A sandwich-type radioimmunoassay for serum ferritin was developed using iron-rich human liver ferritin and evaluated for its clinical usefulness. In young healthy males and females, the mean serum ferritin concentrations were 44 micrograms/L (range 7-158) and 16 micrograms/L (range 4-56), respectively. In anemic patients lower serum ferritin concentrations were found, while in most patients with iron overload serum ferritin concentrations well above 1000 micrograms/L were measured. Comparison of our method with a commercially available radioimmunoassay kit revealed a good correlation, except for sera with very low ferritin concentrations. Comparison with serum iron and transferrin parameters in patients with iron deficiency demonstrated that serum ferritin concentrations might be subnormal in a majority of patients with otherwise normal iron indices. Up to 70% of the ferritin in serum of normal subjects could bind to concanavalin A-Sepharose, indicating its glycoprotein nature. It is concluded that our serum ferritin radioimmunoassay gave reliable results and was useful in the laboratory diagnosis of latent iron-deficiency and in the analysis of the heterogeneity of serum ferritin.
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PMID:Measurement of ferritin in serum: application in diagnostic use. 647 88

A new Enzyme Linked Immuno Sorbent Assay (ELISA) kit for the determination of serum ferritin has been compared with another ferritin kit based on the Immuno Radio-Metric Assay (IRMA) approach, both assays containing similar antibodies. Based on these studies, we found the within-run precision of the ELISA (and IRMA) to have coefficients of variation of 4-10% and 2-6% respectively, over a concentration range of 12-600 micrograms/l. The between-run precision for the same concentration range exhibited a CV range of 9-13% and 7-11% respectively. The sensitivities were found to be 1.4 micrograms/l and 0.9 microgram/l. The mean recovery was 103% for the ELISA procedure. It was found that, using the serum dilution technique, the linearity reached to 1000 micrograms/l. In the ELISA procedure no influence from the so-called "high dose hook effect" was observed. While EDTA-plasma produced 6% lower values than serum in the ELISA technique, no interference from albumin, gamma-globulins and mild haemolysis was observed. Stability problems with the ELISA kit were not encountered. A comparative analysis of multiple specimens demonstrated nearly identical values with r = 0.994 and y = 0.87 x1.01. The quality and ease of operation of the ELISA approach compared with other techniques are discussed. In conclusion it is possible to replace a radio-label in an immunoassay with an enzyme-label with the same degree of reliability and other parameters of quality control exhibited by radioimmunoassays.
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PMID:The influence of the label on the quality of a solid-phase immunoassay: evaluation of a commercial ELISA kit for serum ferritin. 665 46

This study was carried out in twenty-two newborn of birthweight lower than 2 000 g at four and six weeks to determine normal values of serum ferritin in preterm infants and pathological factors who influenced this evolution. Serum ferritin was measured by a kit using a sandwich radioimmunometric method. Elevated values are found in the series of fifteen preterm infants without pathology, with a decreasing evolution. There is no difference in median values at four (266 +/- 91 ng/ml) and six (279 +/- 162) weeks. There is no correlation between serum ferritin values and gestational age nor with hemoglobin values. Serum ferritin values are elevated by inflammatory pathology, blood transfusions and in this case no represent stain pool.
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PMID:[Serum ferritin in premature infants]. 667 22

By means of the RIA-Gnost Ferritin and Spac Ferritin kits, the serum ferritin levels of patients with some urological malignant diseases were quantitated; and, the clinical significance of these values as a tumor marker was discussed. The normal (reference) level in serum obtained by the RIA-Gnost kit was 142.5 +/- 67.5 ng/ml (n = 58) for healthy males and 49.6 +/- 28.8 ng/ml (n = 76) for healthy females. The assay values for 22 cases of bladder cancer by both kits were somewhat lower than the normal range, and the positive rates presented were around 30%, being in rough agreement with each other. The positive rate for 22 patients with prostatic cancer was 45.5% by either method. The assay values obtained by the RIA-Gnost kit was higher and differed significantly (p less than 0.1%) from those obtained on healthy males. Furthermore, the same tendency was found in 6 cases of renal cancer. The assay values of RIA-Gnost were significantly higher (p less than 0.1%) than normal; and, the positive rate was 100% for RIA-Gnost and 67% for Spac . In conclusion, serum ferritin determination especially that by the RIA-Gnost kit proved to be a useful and significant tumor marker for the detection of renal cancer.
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PMID:[Clinical evaluation of serum ferritin levels in urologic cancer (II)]. 667 86

Serum and tissue ferritins were quantitated by a radioimmunoassay kit (SPAC KIT, Daiichi Radioisotope Lab.) and a diagnostic implication of serum ferritins in patients with gynecological diseases was evaluated. In order to investigate the potential use of tumor marker as a feto-placental antigen (protein), ferritin from ovarian cancer was compared with ferritins from normal adult and feto-placental organs. Serum ferritin levels were significantly higher (p less than 0.01) in patients with ovarian adenocarcinoma, Krukenberg's tumor, cervical squamous cell carcinoma and other malignant diseases than in normal women. Among adult organs the kidney and spleen showed the highest and the heart the lowest ferritin content. The ferritin contents of the kidney and spleen were 78.4 micrograms and 76.2 micrograms/g wet weight, respectively and that of the heart was 5.7 micrograms/g wet weight. The ferritin contents of other adult organs ranged from 10 to 25 micrograms/g wet weight. On the other hand the placenta showed the highest and the heart and stomach the lowest ferritin content among feto-placental organs. The ferritin content of the placenta was 7 micrograms/g wet weight. The ferritin contents of other fetal organs were only half as in the placenta. The ferritin contents of ovarian cancers ranged 6 to 8 micrograms/g wet weight and was almost identical to that of the placenta.
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PMID:[Gynecological cancer and ferritin--a study on the carcinofetoplacental ferritin]. 682 63

We evaluated four commercial kits for measuring serum ferritin, based on three techniques: immunoradiometric assay, radioimmunoassay, and enzyme immunoassay. The kits evaluated were those manufactured by Abbott Laboratories, Clinical Assays, Corning Medical, and Ramco. Two of the immunoradiometric kits showed satisfactory results with respect to sensitivity and precision; they should be useful in diagnosing individuals with uncomplicated iron-deficiency anemia. One of the immunoradiometric assay kits, however, showed a high-dose "hook effect," beginning at 10 mg of ferritin per liter. We modified this kit to eliminate this effect, at least to ferritin concentrations of 33 mg/L. (We observed a ferritin value as high as 47 mg/L in one patient.) Results with all these kits did not inter-compare well for ferritin concentrations greater than 300 micrograms/L, a finding that casts further doubt on the controversial use of serum ferritin measurement in cases of iron overload.
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PMID:Three commercial methods for serum ferritin compared and the high-dose "hook effect" eliminated. 685 Nov 5

Serum ferritin level was evaluated as a marker for estimating the effect of the radiotherapy. The ferritin level was determined by a radioimmunoassay (SPAC ferritin kit) in sera obtained serially from 37 patients with various malignant neoplasma before and after radiotherapy. The changes in the ferritin level were investigated in comparison with various clinical findings. The decision for significant changes in the ferritin level was made by the sensitivity which is a difference value necessary to discriminate 2 values at confidence level of 95%. Out of 11 cases with clinical evidence of distant metastasis, the ferritin level was decreased in only one case (9%) and increased or unchanged in the rest. On the other hand, out of 26 cases without evidence of metastasis it was decreased in 9 cases (35%) and unchanged in 12 cases (46%). The ferritin level was either decreased (64%) or unchanged (36%) in all the 11 cases with operation prior to radiotherapy, while out of 26 cases without operation (inoperable) it was decreased in only 3 cases (12%). In all 5 cases with radiosensitive tumors as judged by tumor size the ferritin level was either decreased (40%) or unchanged (60%). Out of 28 cases with radioresistant tumors it was decreased in only 4 cases (14%). All of these results indicated that serial measurements of the ferritin level is usefull for estimating the therapeutic effectiveness in radiotherapy.
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PMID:[Serum ferritin level for estimating the effect of the radiotherapy]. 717 41


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