Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Red cell volume distribution width (RDW-CV) was examined as a means of diagnosing iron deficiency. Iron deficiency was classified as iron deficiency anaemia, prelatent or latent iron deficiency in 1648 students. MCV and RDW-CV (mean +/- ISD) in each group were 89 +/- 4 ft, 12.7 +/- 0.7% in normal individuals, 89 +/- 4 fl, 13.2 +/- 0.8% in prelatent deficiency, 86 +/- 6 fl, 14.0 +/- 1.5% in latent deficiency, and 79 +/- 7 fl, 15.6 +/- 1.7% in iron deficiency anaemia, respectively. Although microcytosis was evident only in iron deficiency anaemia, RDW-CV showed larger values concomitant with the development of iron deficiency. The sensitivity of RDW-CV for the diagnosis of iron deficiency anaemia was 77.1%, and for iron deficiency anaemia and latent deficiency 49.2%, the specificity being 90.6%. In countries with a high prevalence of iron deficiency and low thalassaemia, iron deficiency should be screened by RDW-CV determination without serum iron or ferritin measurements.
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PMID:Change in red blood cell distribution width with iron deficiency. 276 69

Determination of ferritin in serum is valuable for assessing the body iron stores. We have developed a sandwich type enzyme immunoassay for ferritin in human serum and measured serum ferritin concentrations in 179 presumably normal female subjects aged 20 to 88 years. 1) An antibody F (ab')-immobilized solid-phase was first allowed to react with ferritin and then the antibody Fab'-beta-D-galactosidase complex was reacted with the ferritin on the solid-phase. The enzymatic activity bound to the solid-phase was measured by use of 4-methylumbelliferyl beta-D-galactoside as a substrate. 2) An useful range of the assay was from 1 to 100 ng/ml, and the assay was as sensitive as radioimmunoassay. Analytical recoveries of various amounts of ferritin were in the range of 95 to 100%. Between-assay and within-assay coefficients of variation were less than or equal to 12%. The correlation coefficients between values determined by our enzyme immunoassay and two commercially available radioimmunoassay kits (SPAC, GammaDab) were both 0.97. 3) Logarithmic mean values (+/- ISD) of serum ferritin determined by our enzyme immunoassay in 160 normal female subjects were as follows: women 20 to 49 years old, 18.9 (9.2-38.7) ng/ml; women 50 to 59 years old, 36.1 (15.5-84.2) ng/ml; women over 60 years old, 59.2 (31.7-110.8) ng/ml. Twenty six percent of the female subjects aged 20 to 49 years an six percent aged over 60 years showed less than 10 ng/ml levels of serum ferritin, indicating the deficiency of storage iron.
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PMID:[Measurement of serum ferritin by solid-phase enzyme immunoassay: results in normal female subjects of various ages (author's transl)]. 680 11