Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Optimal molar ratios of antibody: ferritin: glutaraldehyde for the preparation of antibody-ferritin conjugates were investigated. The reaction volumes and the antibody concentration were held constant in twenty different reaction mixtures. The effect of five different antibody: glutaraldehyde ratios ranging from 1:25 to 1:400 and of four different antibody: ferritin ratios (1:1 to 1:01) on the yield of antibody-ferritin conjugates was tested in one-step reactions. The 20 different conjugates were tested by a newly developed gel precipitation technique, the inverse fused line rocket immunoelectrophoresis and by counting relative numbers of ferritin monomers and oligomers under the electron microscope. High concentrations of glutaraldehyde produced large heterogeneous precipitates as visualized in the gel technique. The relatively highest yield of ferritin-labeled antibodies without larger conjugates was produced by reacting antibodies, ferritin and glutaraldehyde at molar ratios of 1:1:100. The FRT-antibody conjugates produced at these molar ratios were partly abe to precipitate with their respective antigens.
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PMID:Ferritin-labeling of antibodies by glutaraldehyde. Comparison of conjugates prepared at different antibody: ferritin: glutaraldehyde ratios. 679 Apr 24

Adult Still's disease is a chronic, systemic disease of unknown origin. We describe the case of an otherwise healthy man with an uncommon presentation of Still's disease. A 38-year-old man presented with sore throat, fever, rash and arthritis. Laboratory findings showed that both erythrocyte sedimentation rate and ferritin had increased. Transoesophageal echocardiography revealed a vegetation involving the aortic leaflet. The diagnosis of Still's disease was made after the exclusion of infectious endocarditis, based upon the clinical picture, the high level of ferritin and the follow-up. The patient markedly improved after treatment with prednisone 1 mg. kg-1. This controlled and then progressively reduced the disease; the drug was then withdrawn. This case illustrates that Still's disease can present with endocardial involvement mimicking acute bacterial endocarditis as a first clinical manifestation. The observation suggests that the presence of high ferritinaemia in a patient with some clinical criteria of Still's disease could lead to an early diagnosis.
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PMID:Atypical presentation of adult Still's disease mimicking acute bacterial endocarditis. 874 17