Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The aim of the present investigation was to test a procedure useful for the electron-microscopic in situ identification of the presumptive cariogenic microorganism Streptococcus mutans (serotype d) growing in the human dental plaque. For this purpose, different parameters of an indirect immunohistochemical method were tested in three sets of experiments. In experiment set I, all serological and histochemical procedures were performed en bloc on specimens fixed only with glutaraldehyde before embedding in Vestopal W. Different marking substances, such as ferritin, alkaline phosphatase and horseradish peroxidase (HRP) were tested. The en bloc method using HRP-labelled antibodies was found useful for staining of in vitro-grown bacteria but failed when applied to dental plaque. In experiment set II, ultrathin sections of glutaraldehyde-fixed and glycol methacrylate-embedded in vitro-grown bacteria were section-stained. The bacteria became outlined with a highly electron-dense HRP reaction product which accumulated on top of the cell envelope. The same type of HRP reaction product was found on some bacteria in the 2-day-old dental plaque after section-staining. This system was further tested in a series of controls also using consecutive serial sections. In exp. set III, a number of different stationary and transient oral bacteria were immunohistochemically section-stained. A cross-reaction with bacteria belonging to S.salivarius was discovered and removed by absorbing the anti-S.mutans serotype d serum with S.salivarius (NCTC 8618).
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PMID:Some immunohistochemical experiments aiming at the electron-microscopic in situ identification of a dental plaque microorganism--Streptococcus mutans. 8 15

Since the trophoblast-uterine adhesion is as nearly a universal phenomenon in implantation as can be found, an attempt was made to determine whether or not there was a reduction in cell surface glycoproteins in the rat, as can be observed in the ferret. Neither colloidal iron nor cationized ferritin revealed the type of pattern anticipated for a localized reduction in surface negativity in the imprint of the blastocyst in the implantation chamber. The use of lectin-coated latex beads also proved disappointing in defining regional differences in adhesiveness. However, a number of observations on the changing shape of the implantation chamber, the secretion of periluminal material by decidual cells, and the penetration of the residual basal lamina of the luminal epithelium by the decidual cells were made in the course of these studies. The implantation chamber of the rabbit, in which the blastocyst does not make an imprint, was contrasted with that of the rat. The areas of fusion of trophoblast knobs with uterin epithelial cells shown to be visible by scanning electron microscopy. Finally, some observations on the hypertrophy of maternal epithelial cells to form the uterine plaque in the rhesus monkey are described, and the hypertrophy of endothelial cells to form admirably suited to protein secretion is presented.
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PMID:Comparative aspects of blastocyst-endometrial interactions at implantation. 11 57

The morphology of the transition zone between the terminal plate of the basal body and the 9 + 2 region of the somatic (non-oral) cilium has been examined in Paramecium tetraurelia. Freeze-fracture and thin-section techniques disclosed both membrane specializations and various internal structural linkages. Freeze-fracture material revealed sets of particles interrupting the unit membrane. The more distal of these form plaquelike arrays while the proximal set of particles forms the ciliary "necklace." The plaque regions correspond to anionic sites on the outer membrane surface as revealed by binding of polycationic ferritin. Both the plaque particles and the necklace particles appear to be in contact with outer doublet microtubules via a complex of connecting structures. In the interior of the transition zone an axosomal plate supports an axosome surrounded by a ring of lightly packed material. Only one of the two central tubules of the axoneme reaches and penetrates the axosome. Below the axosomal plate four rings, each approx. 20 nm wide, connect adjacent outer doublets. An intermediate plate lies proximal to these rings, and a terminal plate marks the proximal boundary of this zone. Nine transitional fibers extend from the region of the terminal plate to the plasmalemma. The observations described above have been used to construct a three-dimensional model of the transition region of "wild-type" Paramecium somatic cilia. It is anticipated that this model will be useful in future studies concerning possible function of transition-zone specializations, since Paramecium may be examined in both normal and reversed ciliary beating modes, and since mutants incapable of reverse beating are available.
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PMID:Ultrastructure of the proximal region of somatic cilia in Paramecium tetraurelia. 69 Jan 75

To clarify the association of microglia with senile plaques, the brains from 13 patients with Alzheimer's disease (AD) and 23 nondemented aged controls were investigated immunohistochemically by a double-labeling method using anti-beta-protein antiserum and anti-ferritin antibody, which is a recently reported microglia marker. In addition, a quantitative analysis was performed. The senile plaques which appeared initially in the nondemented aged controls consisted of a diffuse type without any amyloid cores and these were found in the group aged 50-59 years. The great majority of them were found to contain no ferritin-positive microglia. The number and proportion (percentage) of microglia-containing diffuse plaques increased with age. Classical and compact plaques began to appear in the brains of the group aged 70 years and over, and practically all of them contained microglia. These results suggest that microglia are not associated with initial plaque formation, but correlate with amyloid core formation. In AD, the most prominent feature was that the diffuse plaques, which contained either no or only a few ferritin-positive microglia, increased markedly.
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PMID:Increased senile plaques without microglia in Alzheimer's disease. 205 62

An immunostimulatory antigen specific factor (ASF) was found to be secreted by antigen-pulsed macrophages. Macrophages obtained from peritoneal exudate of C57BL/6 mice were pulsed with horse spleen ferritin (HSF). The PM10 ultrafiltration membrane-retained supernatant from the cultures of these macrophages was able to generate helper T cells when introduced into cultures of nylon wool purified T lymphocytes from spleens of C57BL/6 mice. The generation of helper T cells was measured by the cooperation between ASF-induced T cells and splenic B cells in the presence of trinitrophenyl (TNP)-HSF, and subsequently by the anti-TNP plaque forming cell (PFC) assay using TNP-coated sheep red blood cells. The number of PFC obtained from these cultures was significantly higher than the control (T cell cultures without ASF). Background levels of PFC were obtained when the T-B cooperation cultures were challenged with other haptenated antigens (e.g. TNP-BSA) instead of TNP-HSF. In addition, ASF from allogeneic macrophages was unable to facilitate helper cell induction. These results indicated that helper T cell induction by ASF is antigen specific as well as genetically restricted. When small amounts of ASF were injected into syngeneic mice without any adjuvant, specific helper T cells could be obtained from the spleens of these animals which showed that ASF is also active in vivo.
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PMID:Induction of helper T cells by antigen specific factor from macrophages. 214 17

An anomaly of the iron-loading disorder hereditary hemochromatosis is that bone marrow iron stores remain low until later stages of the disease. The possibility that this may be related to a disorder of reticuloendothelial ferritin metabolism was examined by studying ferritin release from mononuclear cells. Ferritin release was measured in peripheral blood mononuclear cells from four patients with hemochromatosis who had not received treatment, from six patients with hemochromatosis who had received treatment, and from 10 age- and gender-matched controls by using a modified hemolytic plaque assay. Ferritin release from the hemochromatotic cells was enhanced when compared with that of controls, and added iron stimulated ferritin release to a comparable degree in both groups. Enhanced ferritin release above matched control values was found both in cells from patients with hemochromatosis with partial phlebotomy who had high serum ferritin values and in cells from patients with hemochromatosis with full phlebotomy who had normal serum ferritin values. The increased ferritin release observed in these studies may signify abnormal reticuloendothelial iron metabolism in hemochromatosis.
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PMID:Ferritin release by mononuclear cells in hereditary hemochromatosis. 278 21

The effect of ferritin on the delayed-type hypersensitivity (DTH) response and Arthus-type reaction as assessed by footpad reaction using methylated human serum albumin, human serum albumin, or sheep red blood cells as antigens was investigated. Intraperitoneally administered ferritin was short acting and suppressed either induction or expression of DTH depending on the time of ferritin injection although it did not inhibit the antibody-mediated inflammatory response, the Arthus reaction. Investigation of ferritin's effect on the primary antibody response revealed that the number of IgG plaque-forming cells (PFC) was moderately decreased but IgM PFC were not. These results indicate that the afferent limb, ferritin selectively suppresses antigen presentation and/or clonal expansion of effector cells of cell-mediated immunity, but not that of the antibody response. Antigen presentation by Ia-positive cells and/or lymphokine-responsive inflammatory mononuclear cells at the efferent limb of DTH is suggested to be affected by ferritin. This conclusion is based upon the observations of successful TDTH effector cell transfer from sensitized but ferritin-treated donors and of successful reversal of ferritin-induced suppression of expression of DTH by supplementing normal bone marrow-derived cells containing Ia-positive ones. Thus our in vivo experimental system might be useful for the differential analysis of immunopathological lesions such as a T-cell-mediated, monocyte-dependent and an antibody-mediated inflammatory lesions.
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PMID:Ferritin selectively suppresses delayed-type hypersensitivity responses at induction or effector phase. 295 43

A lambda gt11 cDNA library was constructed using poly(A)+ mRNA from thyrotropin (TSH)-stimulated Fisher rat thyroid (FRTL5) cells. The library was screened for nonthyroglobulin cDNA sequences by differential plaque filter hybridization using single-stranded cDNA probes synthesized from mRNA prepared from quiescent and TSH-stimulated FRTL5 cells. Thyroglobulin cDNA-containing recombinants in the library were avoided by prehybridizing the TSH probe to excess thyroglobulin cDNA. Of 48,000 clones screened, 60 were chosen as representing mRNA species whose abundance was increased in TSH-stimulated versus quiescent cultures. Southern blot analysis of 9 clones confirmed that the TSH-cDNA probe hybridized to a greater extent to the cDNA inserts than did the control probe. cDNA insert sizes varied between 0.3 kilobase (kb) and 1.0 kb. Northern slot blot analysis using as probes the cDNA of four of these clones (FC4, FC26, FC29, and FC43) demonstrated that TSH stimulation of FRTL5 cells increased the steady state levels of the respective mRNA species by 4-12-fold. For all 4 clones, increases in mRNA levels were apparent within approximately 1 h and were maximal after 14-18 h of TSH stimulation. Determination of the partial nucleotide sequence of these 4 clones confirmed that none was thyroglobulin, thyroid peroxidase, or any other gene previously reported to be stimulated by TSH. Three of the clones bore no homology to any known nucleotide sequence, but FC26 was 85% homologous with human ferritin H. Northern blot analysis using the FC26 cDNA insert as a probe confirmed hybridization to an mRNA species of 1 kb, the known size of ferritin H mRNA. In summary, using the technique of differential plaque filter hybridization, we have identified 4 new genes whose mRNA levels are increased by TSH stimulation of thyroid cells. One of these genes is homologous to human ferritin H.
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PMID:Molecular cloning of cDNA corresponding to mRNA species whose steady state levels in the thyroid are enhanced by thyrotropin. Homology of one of these sequences with ferritin H. 336 67

Serial serum ferritin (SF) levels were measured in 36 patients with neuroblastoma seen at Memorial Sloan-Kettering Cancer Center (MSKCC) between January 1981 and December 1982. The significance of the associations among SF, stage and extent of disease, number of blood transfusions, liver function, serum iron (Fe), total iron-binding capacity (TIBC), and transferrin saturation was investigated. Although a dominant statistical correlation was found between SF and number of blood transfusions, the results suggest that amount of disease contributes to increasing SF levels. Serum ferritin levels increased on average in a linear fashion with number of blood transfusions in patients free of disease or with minimal disease. In patients with bulky disease, this increase was exponential (p value less than 0.01). Application of a reverse hemolytic plaque assay to the analysis of ferritin secretion by cells demonstrates that tumor cells do secrete ferritin in vitro.
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PMID:Ferritin in neuroblastoma. Impact of tumor load and blood transfusions. 402 55

The structure of the sinus walls in the popliteal lymph node of the rabbit was studied with the electron microscope. In the marginal sinus, the endothelial cells are connected by gap junctions, puncta adherentia, and surface specializations characterized by focal approximation of the adjoining membranes without fusion. They possess large numbers of simple and compound uncoated invaginations of the plasma membrane that are closed by a diaphragm with a central thickening. The tissue strands that straddle the lumen of the sinus consist of a fibrous core containing both collagen and elastic fibers, surrounded by endothelial cells identical to those composing the outer sinus wall. Cortical sinuses that run independently of the trabeculae were identified by exploiting the fact that their endothelial cells accumulate lymph-borne ferritin, and their lumen is outlined by horseradish peroxidase administered intravenously. They are lined by a flattened, continuous endothelium and lack luminal strands. The walls of the medullary sinuses consist of endothelial cells and macrophages. The endothelial cells are interconnected by specialized junctions and contain fewer plasmalemmal vesicles than in the cortex; furthermore, dense granules are present in their cytoplasm. Macrophages adhere to the surface of the endothelial cells; typically, none of the junctional specializations that characterize the interface between endothelial cells connect endothelial cells to macrophages. However, at points along the contact region with the endothelium, the plasmalemma of the macrophage is decorated by an attachment plaque of fluffy cytoplasmic material. Sinus endothelial cells slowly accumulate lymph-borne ferritin like vascular endothelial cells elsewhere in the body, whereas macrophages contain both ferritin and engulfed erythrocytes.
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PMID:Structure of the sinus-lining cells in the popliteal lymph node of the rabbit. 407 55


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