Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P02794 (ferritin)
 17,525 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Avian leukosis virus subgroup J (ALV-J) is a new type of virus that mainly induces myeloid leukosis (ML) in chickens. To further elucidate the pathogenesis of ALV-J infection and tumor development, expression profiles from the bone marrow tissue of 15 infected and 18 non-infected birds from a local-breed poultry-farm under naturally infected conditions, were analyzed by suppression-subtractive hybridization. The birds were diagnosed as ML+ (or ML-) by specific ALV-J detection methods, involving serological tests for antigens and antibodies, and RT-PCR to detect viral RNA. A total of 59 partial gene sequences were revealed by differential screening of 496 forward and 384 reverse subtracted cDNA clones. Of these, 22 identified genes, including 8 up-regulated and 14 down-regulated, were related to immune functions, these genes being, MHC B-G antigen, translationally-controlled tumor protein (TPT1/TPTC), transferrin and ferritin, hemoglobin and Carbonic anhydrase. Four of the down-regulated genes were selected for further analysis, in view of their predicted roles in infection and immunity by real-time qRT-PCR, using RNA collected from the same birds as those used for SSH. The four genes were expressed at significantly lower levels (p < 0.001) in ALV-J infected birds than in non-infected ones.
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PMID:Differentially expressed genes in a flock of Chinese local-breed chickens infected with a subgroup J avian leukosis virus using suppression subtractive hybridization. 2163 3

A novel sandwich electrochemical immunoassay was developed for ultrasensitive detection of avian leukosis virus subgroup J (ALVs-J) using graphene quantum dots (GQDs) and apoferritin-encapsulated Cu (Cu-apoferritin) nanoparticles for signal amplification. GQDs were used both for the conjugation of primary ALVs-J antibodies (Ab1), and immobilization of secondary ALVs-J antibodies (Ab2) after compounded with Fe3O4. Cu-apoferritin nanoparticles were first selected to immobilize onto Fe3O4@GQDs hybrid as electroactive probes. After the well-known sandwich-type assembly, Cu was released from the apoferritin cavity, and then detected by differential pulse voltammetry (DPV). Owing to the huge surface area GQDs provided, a considerable number of antibodies were loaded onto the immunosensor, which effectively increased the electrical signal. And the introduction of Cu-apoferritin nanoparticles increased the loading amount of electroactive probes significantly; hence the signal was once again amplified. To embody the signal amplification property of the protocol, the performance of various labels was compared in detail. The immunosensor displayed excellent analytical performance for the detection of ALVs-J range from 10(2.08) to 10(4.50)TCID50/mL with a detection limit of 115TCID50/mL (S/N=3), and the resulting immunosensor also showed high sensitivity, good reproducibility and stability.
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PMID:Electrochemical immunosensor with graphene quantum dots and apoferritin-encapsulated Cu nanoparticles double-assisted signal amplification for detection of avian leukosis virus subgroup J. 2357 Jun 80

A novel signal "on" type of photoelectrochemical biosensor for microRNA-21 hybridization detection was fabricated, where Bi2S3 nanorods were used as photoactive material with a maximum adsorption at 450 nm visible light, hairpin-structure DNA as detecting probe, streptavidin as signal capturing unit and biotin functionalized ascorbic acid loaded apoferritin as signal amplification unit. Hybridization between the probe and the target microRNA-21 was confirmed by the increased photocurrent of the biosensor after electron donor of ascorbic acid was introduced into the detection buffer by digesting the apoferritin by trypsase, indicating that this method could be used fProd. Type: FTPor quantitative measurements, and the discrimination of the complementary from mismatched microRNA-21. Under the optimal detection conditions, the photoelectrochemical biosensor displayed a linear range of 1-5000 fM and a low detection limit of 0.35 fM for microRNA-21 determination. Moreover, the down-regulated expression of microRNA-21 in poultry cells and tissues infecting with avian leukosis viruses was confirmed by directly detecting microRNA-21 in extracted total RNA. This proposed strategy may open a new avenue for the applications of photoelectrochemical biosensor for oligonucleotides detection using visible light irradiation, which could largely reduce the destructive effect of UV light on biomolecules.
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PMID:Photoelectrochemical biosensing platform for microRNA detection based on in situ producing electron donor from apoferritin-encapsulated ascorbic acid. 2414 Aug 33