Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P02774 (Gc-globulin)
 196 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Blood samples from 509 Macushi (3 villages) and 623 Wapishana (11 villages) of Northern Brasil and Southern Guyana have been analyzed with respect to the phenotype and gene frequencies at the following 12 polymorphic loci: ABO, Kell-Cellano, MNSs, Rh, P, Duffy, Kidd, Diego, Lewis, Group-specific component, and the immunoglobulin allotypes of the Gm and Inv systems. The data suggest that 5-6% of the Wapishana gene pool is derived from non-Indians but only 1-2% of the Macushi. Inter- and intratribal genetic distances between villages are calculated for these data in an effort to understand gene flow between the tribes and to account for the unusual distribution of a newly-discovered genetic polymorphism of erythrocyte esterase A thus far limited to these 2 tribes (Neel et al., 1977). The data are puzzling and consistent with the possibility that both the Carib-speaking Macushi and the Arawak-speaking Wapishana have derived the esterase A allele in question from some third group now extinct or thus far undiscovered. Intertribal genetic distances based on gene frequencies at 6 loci are derived for 20 Amerindian tribes (including these 2); the "central" position of these 2 tribes can in part be explained by the active migration matrix connecting them.
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PMID:Genetic studies of the Macushi and Wapishana Indians. II. Data on 12 genetic polymorphisms of the red cell and serum proteins: gene flor between the tribes. 40 46

The denaturant gradient gel electrophoresis (DGGE) method was used in order to simultaneously estimate the genotypes of different factors in a gel plate consisting of one sheet. A genotype analysis of the blood groups (MN, Duffy, Kidd type) and serotype (Gc system) was carried out. DNA samples extracted from the leucocytes of volunteers, whose blood group had already been proven, were used. The PCR amplification fragments were amplified from the gene which controlled each blood group. The primers were designed in order to analyze genotypes with from 1-3 base substitutions in the amplification product. The concentration of the denaturant most suitable for the DGGE method to detect each genotype of the blood groups (MN, Duffy, Kidd type and Gc system) was calculated using a computer simulation method. The denaturant concentration limit of the gel which was suitable for performing a DGGE analysis was determined to range from 15-36%. Electrophoresis was performed at 60 degrees C and with 100 V using this gel on each amplification fragment for 3 hours. The gel after electrophoresis was treated in ethidium bromide in order to detect the DNA band. The genotype (M/M, M/N and N/N) of the MN blood group, the genotype (Fya/Fya, Fya/Fyb and Fyb/Fyb) of the Duffy blood group, the genotype (Jka/Jka, Jka/Jkb and Jkb/Jkb) of the Kidd blood group and each allele (GC*1S, GC*1F and GC*2) of the Gc system were all simultaneously distinguished in one plate. As a result, it was possible to estimate multiple gene polymorphism, while substituting bases 1-3, by PCR, DGGE and ethidium bromide staining. The polymorphic results obtained using this DGGE method is very useful for cases in which a multiple gene analysis is required such as for the personal identification.
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PMID:[Genotyping of blood-groups by denaturant gradient gel electrophoresis (DGGE)]. 1121 56