Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P02774 (
Gc-globulin
)
196
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A CE procedure was established for the nondenaturing separation and identification of the isoforms of the actin-binding human plasma protein
Gc-globulin
. To characterize interactions with globular actin (G-actin), a novel method was developed for the simultaneous qualitative assessment of the binding interaction between the three major isoforms of
Gc-globulin
and G-actin using pre-equilibrium affinity CE and UV detection. Evidence was found that some difference in binding affinity existed among the isoforms, although the quantification of this difference was not feasible by UV detection because of the high affinity nature of the binding. The difference in affinity appeared to be related to the pI of the isoforms; a high pI corresponding to a high affinity. For quantitative binding studies
Gc-globulin
was fluorescently labeled with 5-(and-6)-carboxyfluorescein, succinimidyl ester (CFSE). Data suggested that extensive labeling interfered with actin binding but with moderately labeled
Gc-globulin
it was possible to determine a dissociation constant of K(d) = 21 +/- 1 nM for the binding between labeled
Gc-globulin
and G-actin using pre-equilibrium affinity CE and
LIF
detection.
...
PMID:Affinity capillary electrophoresis for identification and investigation of human Gc-globulin (vitamin D-binding protein) and its isoforms interacting with G-actin. 1838 11
