Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P02749 (
beta2-glycoprotein I
)
836
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Apolipoprotein H
(
ApoH
) is a plasma glycoprotein with its in vivo physiological and pathogenic roles being closely related to its interaction with negatively charged membranes. In this paper, the interaction of
ApoH
with phospholipid vesicles was characterized by (i) detecting the wavelength shift of the fluorescence spectrum of
ApoH
and (ii) measuring the fluorescence quenching extent of
ApoH
by the membrane resident quencher 1-palmitoyl-2-stearoyl-(5-doxyl)-sn-glycero-3-phosphocholine (
DPC
). The observed blue shift upon addition of DMPG vesicles indicated that the tryptophan residues of
ApoH
moved from a polar to a nonpolar environment. The insertion ability of
ApoH
into PG-containing vesicles did not depend on the PG content in a stoichiometric way as did the blue shift, indicating that the negatively charged DMPG does not serve as a specific binding site but rather provides a suitable microenvironment for
ApoH
interaction. The finding that the detachment effect of cations on the blue shift is remarkably different from that on the quenching extent suggests that
ApoH
is capable of existing in two different conformations when membrane-bound.
...
PMID:Intrinsic fluorescence study of the interaction of human apolipoprotein H with phospholipid vesicles. 1041 25
