Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P02749 (
beta2-glycoprotein I
)
836
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Beta 2-Glycoprotein I was shown to be a hydrophilic protein exhibiting no charge shift in the presence of a cationic detergent, but a charge shift in the presence of an anionic detergent. The latter was suggested to be caused by a binding of
beta 2-glycoprotein I
to deoxycholate in the
Triton X-100
/deoxycholate micelles. Quantification by electroimmunoassay of asialo-
beta 2-glycoprotein I
and subfractions of
beta 2-glycoprotein I
gave different results although identical results were obtained in single radial immunodiffusion. Addition of 0.2% (w/v) of deoxycholate to the agarose gels containing
Triton X-100
prior to electrophoresis, however, eliminated these differences. The effect of deoxycholate on the rate of migration of
beta 2-glycoprotein I
was found applicable for electroimmunoassay of the protein. Crossed immunofocusing of plasma from individual donors, electrophoresed in an agarose containing
Triton X-100
/deoxycholate micelles confirmed a postulated variation in the relative composition of subfractions of
beta 2-glycoprotein I
in plasma earlier suggested by Finlayson and Mushinski (Finlayson, J.S. and Mushinski, J.F. (1967) Biochim. Biophys. Acta 147, 413-420).
...
PMID:Addition of deoxycholate in electroimmunoassay and crossed immunofocusing for quantification of beta 2-glycoprotein I and its subfractions. 680 2
