Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P01350 (gastrin)
 9,683 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Hirschsprung's disease (Megacolon congenitum) is characterized by a sustained contraction of a segment of the large intestine and a consequent enlargement of the preceding gut segment. Morphologically, Hirschsprung's disease is characterized by an absence of neuronal cell bodies in the intramural ganglia of the contracted segment. An additional characteristic finding is the presence of enlarged nerve trunks in the submucosa and in the layer separating the circular and longitudinal muscle layers. These nerve trunks contain abundant acetylcholine esterase (AChE)-positive nerve fibers. The nerve fiber hyperplasia together with an increased amount of acetylcholine as well as AChE activity in the aganglionic segment suggests a cholinergic hyperinnervation. There are other reports claiming an adrenergic hyperinnervation in the aganglionic segment. Recent studies on the peptidergic innervation of the afflicted intestinal segment have demonstrated marked reduction in the density of nerve fibers storing vasoactive intestinal peptide (VIP), substance P (SP), enkephalin and gastrin releasing peptide (GRP). The frequency of nerve fibers storing calcitonin gene-related peptide (CGRP) and galanin seems less affected. Interestingly, nerve fibers storing neuropeptide Y (NPY) are more frequent than normally in the aganglionic segment, the circular muscle being penetrated by numerous NPY-containing nerve fibers. Thus, neuropeptides have turned out to be interesting and promising new markers in the histochemical diagnosis of Hirschsprung's disease. Other possibilities for the histochemical diagnosis includes the immunocytochemical demonstration of general neuronal markers such as neurospecific enolase (NEC), neurofilament and chromogranins. Techniques demonstrating the cholinergic and adrenergic hyperinnervation in the aganglionic intestine such as AChE staining and staining for adrenergic nerves are also of interest for the diagnosis.
 ...
PMID:Neuronal markers in Hirschsprung's disease with special reference to neuropeptides. 208 Feb 35

Antibodies to a membrane-bound antigen, localized to the canalicular structures of the parietal cell, are found in most sera of patients with chronic atrophic gastritis and pernicious anemia. In the present study immunoglobulins containing parietal cell antibodies were found to inhibit the activity of H+,K+-adenosine triphosphatase (EC 3.6.1.36) in a tubulovesicular membrane preparation from porcine gastric mucosa. The degree of inhibition correlated to the titer of parietal cell antibodies as assessed by an enzyme-linked immunosorbent assay. The specificity of the enzymatic inhibition was confirmed by the lack of effect of parietal cell antibodies on membrane-bound esterase. A possible interaction of parietal cell antibodies with gastrin binding at the receptor level was investigated in a radioreceptor assay employing 125I-gastrin 1 and gastric mucosal cell suspension from the guinea pig. No blocking capacity was found with immunoglobulins from patients with pernicious anemia as compared with immunoglobulins from healthy controls. The results thus demonstrate a direct inhibitory effect of parietal cell antibodies on the acid producing H+,K+-adenosine triphosphatase of the parietal cell, but also a lack of interaction with the gastrin receptor, and indicate that in the development of hypo/achylia H+,K+-adenosine triphosphatase autoantibodies could have a major pathogenic role.
 ...
PMID:Parietal cell antibodies in pernicious anemia inhibit H+, K+-adenosine triphosphatase, the proton pump of the stomach. 254 Oct 40

The distribution of NO synthase (NOS) immunoreactive nerves and the possible co-existence with other neurotransmitters were investigated in the pig lower urinary tract. NOS immunoreactive nerves were found in the muscle layer, in the lamina propria and around blood vessels. The density of NOS immunoreactive nerves was more prominent in the trigone and urethra than in the detrusor. All parts of the lower urinary tract were supplied by numerous acetylcholine esterase (AChE) positive nerves. The number of adrenergic nerves in the trigone and urethra was moderate to rich, whereas only very few adrenergic nerves were demonstrated in the detrusor. A low to moderate number of nerve fibres containing neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) were observed in the trigone and urethra, while very few were found in the detrusor. A small number of nerves, confined to the trigone and urethra, were stained for calcitonin-gene-related peptide, somatostatin and leu-enkephalin. Nerve fibres exhibiting immunoreactivity to bombesin/gastrin releasing peptide, gastrin/cholecystokinin, substance P or neurokinin A were virtually absent. Co-localization studies revealed that some NOS-immunoreactive nerves also stained for NPY, VIP or AChE. The present study shows that nitrergic nerves are present in the pig lower urinary tract in a density lower than the cholinergic, but higher than any of the studied peptidergic nerves. Coinciding localization of NOS-positive nerves with nerves expressing AChE, VIP and NPY suggests that NO may have a role as a messenger in the lower urinary tract directly and by interaction with other transmitters.
 ...
PMID:Co-existence of nitrergic, peptidergic and acetylcholine esterase-positive nerves in the pig lower urinary tract. 761