Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01350 (gastrin)
 9,683 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Immunoreactive gastrin was present in vagal nerves from cats, dogs, and human beings. The abdominal portion of the vagus contained gastrin in amounts ranging from 16 to 273 pmol/g of nerve tissue (wet weight). The thoracic and cervical portion of the vagi contained only minute amounts of gastrin. Gel chromatography of extracts of human, canine, and feline abdominal vagi monitored by region-specific antisera against heptadecapeptide gastrin and triacontatriapeptide cholecystokinin revealed that the vagal gastrin immunoreactivity predominantly consisted of heptadecapeptide gastrin. In addition, the vagi contained small amounts of the NH2-terminal tridecapeptide gastrin fragment as well as of the putative biosynthetic gastrin precursors, components I and II. No cholecystokinin-like molecules were demonstrable. Immunocytochemical studies demonstrated gastrin-containing nerves in the intestinal wall. The nerves were found to be most numerous in the large and distal small intestine. These findings suggest that heptadecapeptide gastrin may represent a new vagal neurotransmitter.
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PMID:Heptadecapeptide gastrin in the vagal nerve. 2 37

The release of antral gastrin has been examined in human subjects by infusing saline and albumin into their stomachs. Immunoreactive gastrin (IRG) was present in fasting serum but not in fasting gastric juice. Intragastric infusions of saline and albumin induced small but significant (P less than 0.05) rises in the serum concentrations of IRG. There was no significant difference between the magnitude of the serum gastrin responses to these two stimuli. The stimuli induced a rise in the luminal concentration of IRG which was sixfold greater than that in the serum concentration of IRG. The response to albumin was significantly greater than the response to saline (P less than 0.05). The IRG in gastric juice was chromatographically similar to heptadecapeptide gastrin. The stimuli also released IRG in patients with duodenal ulcers. The magnitude of the release into serum and gastric juice in these patients was significantly reduced by antrectomy (P less than 0.05).
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PMID:In vivo release of gastrin into human gastric juice. 2 84

Ultrastructural studies of secretory granules of rat antral G-cells and measurement of serum gastrin level were performed under the condition of fasting and administration of alkaline solution into the stomach. On electron micrographs, no qualitative difference was observed among those experimental groups. However, morphometrical analysis revealed significant quantitative differences. The population density of secretory granules of the rats treated once with alkali first increased and then decreased reaching that of the fasted group, while that of the repeatedly treated group remained nearly equal to the maximum value. The average sectioned surface area of secretory granules tended to decrease for 1.5 h after the stimulation but the differences was not significant among those groups. From the results obtained at present, responding to chemical stimulation such as pH changes in the antrum, it seems probable that not only exocytosis but also migration of secretory granules from supra- and/or para-nuclear portion to the basal portion of the cell occurs rapidly in G-cells and that both these processes are inhibited immediately by antral acidification. Moreover, the present results apparently indicate that under the condition of no antral acidification G-cells have a capacity of secreting gastrin for a fairly long time, such as 4--8 h, responding to adequate stimulus. These findings are strongly suggestive of the existence of a capacious pool of granules in the supra- and/or para-nuclear cytoplasm or of fairly speedy production of secretory granules in the Golgi area.
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PMID:Quantitative electron miscroscopic studies on the kinetics of secretory granules in G-cells. 2 98

A method is described for the simultaneous evaluation of the pancreatic, the biliary and the gastric secretion in long-term experiments. Under these conditions, the pancreatic basal secretion is characterized. The pancreatic basal secretion is a very variable reference value that is influenced by endogenous stimuli. The upper threshold value for the secretion rate was 2.2 ml/15 min. Under the influence of spontaneous biliary secretion, the protein concentration amounts to a characteristic value of relatively unimportant variance. If the pancreatic basal secretion is used as a reference value, the spontaneous secretions of the biliary ducts (gastrin release from the duodenum?) and of the stomach (secretin release from the duodenum) and be taken into account. A further disturbing factor interfering with both the pancreatic and the gastric basal secretion is to be neglected.
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PMID:[Characteristics of pancreatic basal secretion in the dog]. 2 16

The gastric acid, pepsin, and secretory volume output in response to a mixed meal were measured in six patients with Zollinger-Ellison syndrome caused by a gastrin-producing tumour proved subsequently at surgery. The patients were all normocalcaemic, and none had previous abdominal surgery. In four of the six patients, ingestion of the meal markedly inhibited the gastric secretory output, which decreased to below fasting levels, returning later to basal values. In two other patients, whose fasting acid output was considerably lower, the secretory output increased after the meal, but some inhibiton of gastric secretion was also apparent for variable intervals of time. The serum gastrin concentration in all patients remained essentially unchanged or increased after the meal. Two patients were restudied after successful removal of the duodenal gastrin-producing tumour, and in each the normal gastric secretory and gastrin-releasing responses were completely restored. Our studies suggest that, in patients with the Zollinger-Ellison syndrome caused by a gastrinoma, physiological regulatory mechanisms triggered by food reduce the continuous stimulation of gastric secretion caused by their tumoural hypergastrinaemia.
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PMID:Pathophysiological responses to meals in the Zollinger-Ellison syndrome: I. Paradoxical postprandial inhibition of gastric secretion. 2 28

In 10 healthy subjects and 10 duodenal ulcer patients the intestinal phase of gastric acid secretion was studied by intraduodenal infusion of a 10% liver extract meal (pH 7) at 400 ml/h for three hours. A gastroduodenal double lumen tube with two balloons was used to block the pylorus and to prevent duodenogastric reflux. Gastric acid response to a duodenal meal of liver extract reached a peak at the end of the first hour of infusion of the extract and was then followed by a relatively well-sustained plateau. When the figure was normalised as a percentage of peak response to pentagastrin it was about 45% in healthy subjects and 63% in duodenal ulcer patients. Serum gastrin concentration increased significantly during a duodenal meal of liver extract only in duodenal ulcer patients and not in healthy subjects. The combination of the duodenal meal of liver extract with pentagastrin infusion resulted in a significantly greater increase in acid output in duodenal ulcer patients than in healthy controls. Duodenal perfusion with a liver extract meal in which the pH was gradually decreased caused a pH-dependent reduction in acid output, but not in serum gastrin, both in the duodenal ulcer patients and in healthy subjects. This study shows that the intestinal phase in man results in a potent gastric acid stimulation which is pH-dependent, greatly augmented by pentagastrin, and more vigorous in duodenal ulcer patients than in healthy controls.
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PMID:Intestinal phase of gastric secretion in patients with duodenal ulcer. 2 29

Components of a histochemical method for demonstrating carbonic anhydrase activity have been investigated quantitatively. It was found that it is not necessary to use free-floating sections provided the reaction is done in a reaction medium of controlled depth. This permits the use of normal cryostat sections on glass slides, so making this technique applicable to the cytochemical bioassay of gastrin. The better control of the pH of the reaction, and changes in the concentration of phosphate and of cobalt, have resulted in a quantitatively reproducible reaction in the parietal cells of guinea-pig fundus. The reaction product is measured by microdensitometry. The specificity of the carbonic anhydrase reaction has been tested by the response elicited by gastrin acting on the parietal cells in vitro and by the use of acetazolamide.
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PMID:A quantitative cytochemical method for measuring carbonic anhydrase activity. 2 56

A double-blind trial was carried out in 30 patients with peptic ulcers to assess the effects of treatment with a gastrin-receptor antagonist, proglumide, compared with a histamine H2-blocker, cimetidine. Patients received either 1200 mg proglumide or 1200 mg cimetidine per day for 28 days. The results showed that both drugs significantly reduced clinical symptoms and gastric secretion. In patients treated with cimetidine there was a significant increase in blood gastrin levels and marked hypertrophy and hyperplasia of the antral mucosa was observed in almost all patients. No such changes were found in the patients treated with proglumide.
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PMID:Double-blind clinical comparison between a gastrin-receptor antagonist, proglumide, and a histamine H2-blocker, cimetidine. 2 18

Differential stimulating effects on the parietal cell of dogs by calcium, magnesium and sodium were noted only at an alkaline pH. Acid inhibited the parietal cell, and antral perfusion did not cause gastrin stimulation at any pH level. The pH of the milieu of the parietal cell appears to be a more critical regulator of acid secretion than physiologic antral gastrin variation.
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PMID:Cation and hydrogen ion effect on canine acid and gastrin output. 2 82

1. Twelve gastric-cannulated litter-mate pigs were used to study secretion and proteolytic activity in the stomach of suckling and early-weaned pigs in relation to age and food intake. 2. Results demonstrate that from the first observation at day 8, piglets were able to secrete acid. pH and acid concentration did not change during the first 4 weeks of life. 3. Proteolytic activity was low during the first 2--3 weeks of life and rapidly increased thereafter. 4. Two phenomena differentiated suckling pigs from pigs given dry cow's milk: (1) a low buffering capacity the gastric contents, beginning 1 hr after feeding the dry cow's-milk formula, results in a low total acid concentration in the weaned pigs and (2) the increase in proteolytic activity in relation to the age is much more pronounced in the artificially-reared pigs. 5. These two phenomena are discussed and related to the formation of a hard casein clot in the stomach of the cow's-milk-fed pigs, which has a long retention time and stimulates gastrin release.
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PMID:Gastric secretion in suckling pigs and early-weaned pigs given a dry cow's-mild formula ad lib. 2 7


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