Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01350 (gastrin)
 9,683 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Intestinal mucosal biopsy specimens processed during the past 25 years were used to example the ultrastructural characteristics of intestinal endocrine cells. The cells were defined on the basis of morphologic criteria and, when feasible, with specific antisera and immunogold staining. The hypothesis was that each endocrine cell, once well defined, should be identifiable on the basis of standard morphologic criteria not requiring specific immunostaining. This was not the case, D, G, EC1, EC2, ECn, D1, and intestinal gastrin cells have characteristic secretory granules and, when sufficient granules are present, can be identified consistently on the basis of morphologic criteria. Absolute identification of D, G, IG, and TG cells requires staining with specific antisera, a condition easily obtainable only for D, G, and IG cells. D1, EC1, EC2, and ECn cells must be identified morphologically until secretory products specific for each of these cells are identified. I, L, N, and K cells are remarkably similar in appearance and must be distinguished by specific staining. Mo, S, and P cells were not identified by either morphologic appearance or immunostaining. It is suggested that a cell similar to the D1 cell but with exceptionally small granules may be the P cell. Absolute identification of intestinal enteroendocrine cells by electron microscopy requires specific staining. The characteristic appearance of the secretory granules of many of these cells (D, G, EC1, EC2, ECn, D1, and IG) permits morphologic identification when numerous secretory granules are present.
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PMID:Electron microscopic definition of intestinal endocrine cells: immunogold localization and review. 201 67

An argentaffin carcinoid tumour of the caecum which contained serotonin (167 micrograms/g) and consisted predominantly of EC1-cells, was analysed for the presence of peptides using immunohistochemical, biochemical and pharmacological methods. A very high content of 3.9 micrograms/g of immunoreactive substance P was found. The distribution of cells staining positively for substance P matched that of cells containing serotonin. While some immunoreactive somatostatin (3.2 ng/g) was present in the tumour, neurotensin, glucagon, gastrin, and motilin were not found. Part of the substance P immunoreactivity measured most likely represents authentic substance P: it behaved like substance P in two chromatographic systems and in two bioassays, and its activity on the guinea pig ileum was abolished by specific tachyphylaxis towards substance P.
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PMID:Substance P in the argentaffin carcinoid of the caecum: biochemical and biological characterization. 616 90