Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01350 (gastrin)
 9,683 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Compared with conventional light microscopy, fluorescence visualization of parietal cells was found to be of great advantage after staining with the acid dye Eosin or Azophloxine in combination with Hematoxylin or Periodic Acid Schiff (PAS). All these staining combinations gave a satisfactory result in the body area, whereas PAS-Eosin was superior in pyloric glands. Direct or indirect immunohistochemical method with human parietal cell antibody could also be used for distinct demonstration of parietal cells in paraffin-embedded rat and human gastric mucosa. Results of immunohistochemistry confirmed the reliability of parietal cell enumerations based on fluorescence staining with the acid dyes. Paired visualization of parietal cells and gastrin cells (G cells) in the same tissue section was achieved immunohistochemically. This methodological approach is a prerequisite for a detailed study of the morphological relationship of these two cell types in the transitional zone between the gastric body and the pyloric antrum.
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PMID:Fluorescence staining of gastric mucosa. A study with special reference to parietal cells. 7 85

The gastric mucosa is most susceptible to stress that has been shown to induce mucosal damage in humans and animals. This study aims to explore the underlying mechanisms of partial sleep deprivation, as a source of psychophysiological stress, on gastric functions and its effect on mucosal integrity. Sprague-Dawley rats were partially sleep deprived (PSD) for 7 or 14 days by housing inside slowly rotating drums. Gastric tissues and plasma were sampled at the end of the sleep deprivation periods and mucosal lesion scores were evaluated. Morphological examination was performed after Hematoxylin and Eosin staining. Plasma levels of noradrenaline, adrenaline, gastrin, histamine and somatostatin were determined with enzyme immunoassays. Gastric acidity was measured with acid-base titration in pylorus ligated rats. Gastric mucosal blood flow was evaluated with Laser Doppler Flowmetry. It was found that gastric lesions were induced in about 30%-50% of the PSD rats. Gastric acidity as well as plasma levels of noradrenaline, gastrin and histamine were elevated. Gastric mucosal blood flow and plasma somatostatin level were on the contrary reduced, especially in rats with PSD for 14 days. It is concluded that partial sleep deprivation compromises gastric mucosal integrity by increasing gastric acidity, plasma levels of noradrenaline, gastrin, histamine, and decreasing gastric mucosal blood flow. These results provided experimental evidence on the gastric damaging effects of PSD and it could be one of the risk factors contributing to gastric ulcer formation.
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PMID:Partial sleep deprivation compromises gastric mucosal integrity in rats. 1586 6