Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01275 (glucagon)
 26,492 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Partial pancreatic duct obstruction due to gallstone migration has been suggested as an important step in the pathogenesis of gallstone, pancreatitis. Since gallstone migration often follows a meal, pancreatic secretory stimulation is also present. Utilizing the isolated perfused canine pancreas, an experimental model of gallstone pancreatitis was developed by partial obstruction of the main pancreatic duct and secretin stimulation (POSS). In control glands (n=6) perfused for a four-hour period, gross appearance remained normal, weight gain (8 g) was minimal, and mean amylase (875 Caraway units/dl) remained within normal limits. POSS glands (n=9) became markedly edematous during the perfusion period, with significant weight gain (47 g) and hyperamylasemia (7200 Caraway units/dl). Steroid-treated (n=6) and Trasylol-treated (n=6) POSS glands became edematous, and mean weight gain and hyperamylasemia were similar to those seen in untreated POSS glands. Glucagon-treated POSS glands (n=6) became edematous, but mean weight gain (24 g) was significantly decreased compared with that of untreated POSS glands. Mean amylase elevation was unchanged (8536 Caraway units/dl). POSS glands treated with albumin (n=6) remained normal in gross appearance, mean weight gain (12 g) was minimal and mean amylase (3120 Caraway units/dl) was significantly decreased compared to that of untreated POSS glands. The failure of Trasylol to ameliorate the injury response and the effectiveness of albumin were interpreted as evidence against enzyme extravasation and for capillary injury as the initial step in the pathogenesis of gallstone pancreatitis.
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PMID:Experimental gallstone pancreatitis. Pathogenesis and response to different treatment modalities. 617 93

A specific and sensitive radioimmunoassay of human vasoactive intestinal polypeptide using synthetic VIP as standard preparation and antiserum to synthetic VIP R 502 (Yanaihara et al. 15) is described. No crossreactions with a number of other gastrointestinal hormones such as glucagon, secretin, GIP, HPP or substance P was detected. Aprotinin (Trasylol, Bayer) or heparin had no influence on the antigen--antibody reaction. High concentrations of sodium or potassium chloride in plasma, or hyperlipoproteinemia, did not interfere with the antiserum in the described system. Basal plasma concentration of VIP in 18 females (means +/- sem = 24.05 +/- 1.79 VIP/l) and 20 males (means +/- sem = 24.11 +/- 1.91 pmol/l VIP) showed no sex specific variations. During gastroscopy plasma VIP levels were significantly higher than basal values, showing a peak secretion when the gastric antrum was inspected. During the entire colonoscopic examination, VIP levels were significantly higher than basal values. Endoscopic examination may possibly liberate VIP from the VIP containing cells which can be found throughout the gastrointestinal tract.
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PMID:Radioimmunoassay for vasoactive intestinal polypeptide (VIP) in plasma before and during endoscopic examinations. 712 38

The stability of glucagon immunoreactivity in plasma samples, as measured with antiserum 30K, has been investigated. Post-prandial blood samples were collected and processed both with added Trasylol at 4 degrees C and without Trasylol at room temperature. Incubation at room temperature for up to 7h without added Trasylol did not result in significant loss of 30K-immunoreactive glucagon. Blood samples both from non-diabetic and diabetic persons could be collected and centrifuged at room temperature and stored at -20 degrees C without Trasylol for more than 2 months without loss of 30K-immunoreactivity, as compared to Trasylol-containing controls kept at 4 degrees C during handling. The distribution of 30K-immunoreactive glucagon over the various components, obtained after chromatography of plasma on Ultrogel AcA54, was not significantly altered when blood samples, both from normal persons and from patients with chronic renal failure, were processed at room temperature and subsequently stored at -20 degrees C without addition of Trasylol. Our results indicate that collection and centrifugation of blood at room temperature without the addition of a proteinase inhibitor and subsequent storage at -20 degrees C does not result in loss of 30K-immunoreactive glucagon.
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PMID:On the stability of immunoreactive glucagon in plasma samples. 728 91


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