Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01275 (glucagon)
 26,492 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The activity of hepatic lysine-2-oxoglutarate reductase was determined in white female rats maintained on one of two dietary lysine levels (0.23 and 0.54% in diets A and B, respectively). The high dietary lysine increased the enzyme activity (p less than 0.05) twofold compared with the activity obtained with the diet A (67 vs. 27 mumol saccharopine/30 min/g liver protein). Repeated intraperitoneal injection of glucagon (259 microgram/100 g body weight/day) showed a short initial drop in the enzyme activity, followed by a marked rise, which remained above the basal level after withdrawal of the drug. Repeated injections of cycloheximide increased the enzyme activity, and the findings suggested that the apparent increase was due to the inhibition of the system responsible for the degradation of the enzyme. The individual free amino acids of the livers varied as a function of treatment. The ratio of E/N amino acids was found to be a good measure of the physiological condition of the animal, with the lowest value in animals treated with cycloheximide.
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PMID:Rat hepatic lysine-2-oxoglutarate reductase activity. Induction by lysine, glucagon and cycloheximide administration. 61 19

L-Lysine-2-oxoglutarate reductase (EC 1.5.1.8, NADP) in the liver of adult rats increased 4-5 times when the animals were treated with alloxan. In diabetic rats injection of insulin or adrenalectomy prevented the increase in enzyme activity. The activity of the similar enzyme in kidney was not changed by these treatments. The enzyme activity in primary cultured adult rat hepatocytes was also induced by addition of dexamethasone and glucagon together, and glucagon could be replaced by dibutyryl cyclic AMP. Insulin inhibited the induction. The hormonal induction was also inhibited by actinomycin D and by cycloheximide. During development of rats, fetal liver showed very low activity, but the activity appeared on day 1 after birth and then increased rapidly, reaching the adult level by day 5. The activity of the kidney enzyme increased more slowly and reached adult level 1 month after birth. Intra-uterine injection of glucagon caused precocious induction of the liver enzyme in fetuses. These results indicate that the activity of L-lysine-2-oxoglutarate reductase in the adult liver and in part in neonatal liver also, in controlled by both glucagon and glucocorticoid.
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PMID:Induction of L-lysine-2-oxoglutarate reductase by glucagon and glucocorticoid in developing and adult rats: in vivo and in vitro studies. 701 89