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Query: UNIPROT:P01189 (
beta-endorphin
)
21,003
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Relaxin-3/
RXFP3
signalling is proposed to be involved in the neuromodulatory control of arousal- and stress-related neural circuits. Furthermore, previous studies in rats have led to the proposal that relaxin-3/
RXFP3
signalling is associated with activation of the hypothalamic-pituitary-adrenal axis, but direct evidence for
RXFP3
-related actions on the activity of hypothalamic
corticotropin
-releasing hormone (CRH) neurons is lacking. In this study, we investigated characteristics of the relaxin-3/
RXFP3
system in mouse hypothalamus. Administration of an
RXFP3
agonist (
RXFP3
-A2) intra-cerebroventricularly or directly into the paraventricular nucleus of hypothalamus (PVN) of C57BL/6J mice did not alter corticosterone levels. Similarly, there were no differences between serum corticosterone levels in Rxfp3 knockout (C57BL/6J
RXFP3TM1
) and wild-type mice at baseline and after stress, despite detection of the predicted stress-induced increases in serum corticosterone. We examined the nature of the relaxin-3 innervation of PVN in wild-type mice and in Crh-IRES-Cre;Ai14 mice that co-express the tdTomato fluorophore in CRH neurons, identifying abundant relaxin-3 fibres in the peri-PVN region, but only sparse fibres associated with densely packed CRH neurons. In whole-cell voltage-clamp recordings of tdTomato-positive CRH neurons in these mice, we observed a reduction in sEPSC frequency following local application of
RXFP3
-A2, consistent with an activation of
RXFP3
on presynaptic glutamatergic afferents in the PVN region. These studies clarify the relationship between relaxin-3/
RXFP3
inputs and CRH neurons in mouse PVN, with implications for the interpretation of current and previous in vivo studies and future investigations of this stress-related signalling network in normal and transgenic mice, under normal and pathological conditions.
...
PMID:Relaxin-3/RXFP3 signalling in mouse hypothalamus: no effect of RXFP3 activation on corticosterone, despite reduced presynaptic excitatory input onto paraventricular CRH neurons in vitro. 2831 51
