Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01189 (beta-endorphin)
 21,003 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We studied the effects of psychosocial stress (S) and diazepam (D) on plasma lipids, adrenocorticotropin (ACTH), and corticosterone (B) levels of cockerels fed an atherogenic diet (AD) consisting of 2% cholesterol plus 5% cottonseed oil added to plain mash (PM). Seventy-six eight-week-old DeKalb cockerels were randomly assigned to the following groups: I. PM; II. PM + D; III. PM + S; ;IV. PM + S + D; V. AD; VI. AD + D; VII. AD + S and VIII. AD + S + D. S was induced by housing two birds to a cage and pairing them to a different bird daily. D was administered daily by gavage. Plasma ACTH and B levels were analyzed by RIA. Aortic atherosclerosis was grossly graded on a scale of 0-4 and also by gravimetric planimetry. After 10 weeks: 1. S birds had a significantly higher incidence and severity (p less than 0.04) of aortic atherogenesis and elevated ACTH and B levels (p less than 0.001) compared to unstressed PM groups. 2. AD significantly elevated the plasma levels of cholesterol, triglycerides, and the lipoprotein cholesterol that was precipitated by heparin-manganese (LDL-C + VLDL-C), compared to initial and/or PM levels (p less than 0.001). AD birds had a greater incidence and more severe aortic lesions in comparison to PM groups (p less than 0.002). Plasma hormone levels were significantly lower in birds fed AD alone compared to controls and stressed birds. 3. D significantly reduced the severity of aortic atheroma as well as decreased hormone levels in all treated groups (p less than 0.001). Therefore, we conclude that aortic atherosclerosis in cockerels can be induced by S and/or AD, and D can markedly reduce atherogenesis under these conditions. Since both AD and D decreased plasma ACTH and B levels, the anti-atherogenic action of D in these birds does not seem to directly involve these pituitary-adrenocortical hormones.
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PMID:Effects of diazepam, psychosocial stress and dietary cholesterol on pituitary-adrenocortical hormone levels and experimental atherosclerosis. 185 May 93

Obesity, the most frequent nutritional problem throughout the rich nations, can have a vast and significant influence on different aspects of endocrinology, in particular on ovulation disfunction, on hyperandrogenemia, on hormone-sensitive carcinomas. Our study proposes to value the response to adrenal cortex to stimulation with adrenocorticotropin (ACTH) hormone in obese patients, with particular attention to the behavior of adrenocortical androgens and their precursor. We recruited 30 female patients so divided: 12 obese, nonhirsute, eumenorrheic patients (group A); 10 normal weight, hirsute patients in situation of secondary amenorrhea (group B); 8 normal weight, nonhirsute, eumenorrheic patients (group C). Cortisol, progesterone, 17 OH progesterone, dehydroepiandrosterone sulfate, androstenedione, testosterone were measured at 60, 120, 180, 240, 300 min during continual infusion i.v., for 5 h, of ACTH 1-17 at 100 mcg dose, in physiological sodium chloride solution. All the women with monthly menstruation were studied between the IV and VIII day of their cycle. In the patients with secondary amenorrhea the value of basic progesterone was used to completely exclude an eventual luteal phase and the relationship LH/FSH was so as to logically exclude a diagnosis of polycystic ovary. This exclusion was also confirmed from the report of the ultrasonography. The basic concentration of hormone dosage is not significantly different between the patients of the three groups, except for T. This hormone is different because it is found to be significantly (p < 0.01) increase in the hirsute patients, in respect of the patients in group A and group C. Also P and 17OHP have been found to be higher, if only in insignificant measure, in hirsute patients.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Probable role of obesity on the adrenal response to acute stimulation with adrenocorticotrophic hormone in eumenorrheic and hirsute, non-eumenorrheic women]. 823 17

We describe the construction and screening of a random peptide library displayed by filamentous phage. The peptides are expressed in multiple copies on the filamentous phage M13 as amino-terminal fusions with the major coat protein, the product of gene VIII. These libraries are efficiently screened for reactive peptides, using a combination of panning in solution followed by a plaque lift assay. Advantages of this system are that both high- and low-affinity phage clones are simultaneously identified and the analysis of non-reactive phage is minimized. The vector system utilized to construct this library enables it to be used for the construction of peptide libraries employing a combinatorial cloning strategy. This feature makes it especially suitable for construction of peptide libraries using codon-based oligonucleotide synthesis. The vectors also allow rapid optimization and modification of lead peptides by codon-based mutagenesis. A 20-amino acid long random peptide library of 1 x 10(9) members was constructed and screened for peptides that bound to (i) a monoclonal antibody recognizing the amino-terminus of beta-endorphin; (ii) a monoclonal antibody recognizing a peptide epitope derived from the v-ros oncogene product; and (iii) the constant region of murine IgG2b. The approach described here provides a means for the construction of customized libraries that can be screened with a variety of target molecules.
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PMID:A combinatorial method for constructing libraries of long peptides displayed by filamentous phage. 923 93

Corticotropin releasing factor is a 41 amino acid peptide that is present in afferent systems that project to the cerebellum. In the adult, this peptide modulates the activity of Purkinje cells by enhancing their responsiveness to excitatory amino acids. Two different types of corticotropin releasing factor receptors, designated type 1 and type 2, have been identified. The purpose of this study is to use immunohistochemistry to identify which corticotropin releasing factor receptors are present in the cerebellum of the adult mouse and to determine their cellular distribution. Receptor type 1 immunostaining is present throughout all lobules of the cerebellar cortex. Distinct labeling is present over the somas of most, if not all, Purkinje cells as well as the primary dendrites of Purkinje cells located at the base of vermal folia. In vermal lobules V, VI, VIII and IX numerous glial fibrillary acidic protein immunoreactive processes, oriented radially in the molecular layer, also are immunoreactive for receptor type 1. In the granule cell layer, scattered type 1 immunoreactive puncta are present throughout most cerebellar lobules. Receptor type 2 immunoreactive puncta are present throughout the molecular layer in all lobules. In addition, scattered basket and/or stellate cells, identified with a GABA antibody, are immunopositive for the type 2 receptor. In the Purkinje cell layer, the type 2 receptor immunolabeling is confined to the basal pole of the Purkinje cell including the initial axonal segment. In the granule cell layer, labeling is present over large cell bodies, and their initial axonal segments. These are likely to be Golgi cells, based on their co-staining with GABA. Finally, numerous elongated processes within the white matter, which are likely to be axons, also are type 2 immunoreactive. These data indicate that both types of corticotropin releasing factor receptor are present in the mouse cerebellum. However, the unique distribution of the two types of receptor strongly suggests a differential role for corticotropin releasing factor in modulating the activity of neurons, axons and glial cells via cell-specific ligand-receptor interactions.
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PMID:Cellular localization of corticotropin releasing factor receptors in the adult mouse cerebellum. 1111 57