Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P01189 (
beta-endorphin
)
21,003
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In this method for rapid separation of
beta-endorphin
(beta-EP) and beta-lipotropin (
beta-LPH
) in plasma, most of the plasma proteins in a 0.5- to 2.0-mL sample of plasma are precipitated with acetonitrile at pH 4.7. beta-EP and
beta-LPH
in the supernate are completely separated by liquid chromatography on a cation-exchange column, with gradient elution with volatile buffers, and are eluted in 1.5- to 2.0-mL volumes. After lyophilization, the redissolved beta-EP and
beta-LPH
are determined by radioimmunoassay. The respective mean concentrations of immunoreactive beta-EP and
beta-LPH
in plasma of nonpregnant women were 4.0 (SD 2.0) and 5.1 (SD 3.0) pmol/L; during labor, 33.3 (
SD 25
) and 69.6 (SD 41) pmol/L; and in venous plasma from umbilical cords after spontaneous labor, 38.6 (SD 18) and 38.7 (SD 19) pmol/L.
...
PMID:Determination of plasma beta-endorphin and beta-lipotropin by cation-exchange liquid chromatography and radioimmunoassay. 315 1
