Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P01189 (beta-endorphin)
 21,003 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. Genetically obese Zucker rats (fa/fa) contain 2-3 times higher activities mono- and diacylglycerol lipases in their spinal cords than their lean littermates. 2. When rats were exercised (1 hr daily, 5 days/week) on a treadmill for 6 months, there was a decrease of about 30% (P less than 0.05) in the activities of mono- and diacylglycerol lipases in lean rats but not in obese animals. 3. High activities of lipases in Zucker obese rats may be related to the elevated levels of beta-endorphin present in these animals. 4. The activities of arylsulfatase, beta-N-acetylhexosaminidase and alkaline phosphatase, tested to check the stability of spinal cord extracts, were similar in lean and obese rat spinal cords.
 ...
PMID:Mono- and diacylglycerol lipases in spinal cord of lean and obese Zucker rats. 295 45

Treatment with the enzyme arylsulfatase in vivo selectively attenuated the effect of analgesia induced by morphine, beta-endorphin or ethylketocyclazocine but not that induced by Sandoz FK33824 or D-ala2-D-leu5-enkephalin. The effect on morphine analgesia was indicated both by an increased morphine ED50 in the presence of a fixed dose of naloxone and by a decreased naloxone ED50 in the presence of a fixed dose of morphine. Arylsulfatase treatment in vivo also selectively affected in vitro ligand binding; Bmax values of the low affinity binding site of dihydromorphine, naloxone, D-ala2-D-leu5-enkephalin, D-ala2-met5-enkephalinamide and ethylketocyclazocine were decreased significantly while the Bmax values of the high affinity sites as well as the KD values of both the high and low affinity sites were affected little or not at all. The data suggest that the change induced by the enzyme may have been due to the alteration of certain constituents of the low affinity opiate binding site.
 ...
PMID:Differential modification of opiate receptor activity by arylsulfatase treatment. 613 34

Treatment of the isolated mouse was deferens with the enzyme arylsulfatase (E.C. 3.1.6.1) had an effect on the ability of this tissue to respond to various opiates. It increased the IC50 values and slopes of their dose-response curve for enkephalins and their analogs, and shifted to the right the curves for FK33824, levorphanol and normorphine. There was no effect on the action of etorphine, beta-endorphin or dynorphin. With morphine there was a biphasic effect, IC50 values increasing at low enzyme concentrations and decreasing at high enzyme concentrations. A further comparison of arylsulfatase effects on morphine and on D-Ala-2-D-Leu5-enkephalin indicated that the morphine effect, unlike the D-Ala-2-D-Leu5-enkephalin effect, could not be reversed by washing and that morphine, unlike D-Ala2-D-Leu5-enkephalin, became much less sensitive to naloxone antagonism. The observed modifications in the shape of the dose-response curves indicate that the effect of the opiates in the mouse vas deferens is more complex than that expected through the occupation of a simple receptor. There is either more than one type of functional receptor for each agonist or only one receptor, which can interact with every drug in different ways; this complexity is discussed in terms of various possibilities, including fractional occupancy, positive cooperativity and multiple sites.
 ...
PMID:Functional opiate receptor in mouse vas deferens: evidence for a complex interaction. 631 79