Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01189 (beta-endorphin)
 21,003 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The time course of change in the mRNA concentrations of the proto-oncogene c-fos and pro-opiomelanocortin after two different methods of morphine treatment was examined in SH-SY5Y human neuroblastoma cells. In a repeated treatment design, SH-SY5Y cells exposed to morphine sulfate (MS) for 12 h or more were periodically given fresh morphine (10 or 1 muM). In a single-dose design, 10 muM MS was added to the flasks at designated times without changing the medium. Slot-blotting hybridization analysis of total cellular RNA using a [(32)P]-fos cDNA probe revealed that repeated morphine treatment caused both an early transient induction of c-fos and a later prolonged increase in c-fos. Single treatment with morphine caused only a transient and rapid induction of c-fos. Slot-blotting hybridization with a [(32)P]-POMC cRNA probe revealed that POMC mRNA was significantly activated at 6 h and remained significantly elevated up to 7 days in the cells with repeated morphine treatment. In the single-dose experiments, however, the POMC mRNA was not significantly elevated at 2 days or less. It was significantly activated at 6 days, but at a much lower level than that seen in the repeated-dose design. These results indicate that repeated exposure to morphine induces a prolonged activation of c-fos mRNA which may be functionally related to the significant activation of POMC mRNA in SH-SY5Y cells.
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PMID:Prolonged Activation of c-fos and Optimal Activation of Pro-opiomelanocortin mRNA after Repeated Morphine Exposure in SH-SY5Y Cells. 1991 4

Geese have the strongest tendency toward broodiness among all poultry. The mechanisms initiating broodiness within the goose hypothalamic-pituitary-gonadal axis (HPGA) are still unclear. Here, we reported the transcriptome differences between laying and initial nesting within the HPGA tissues of geese. We constructed a unigene database based on HPGA tissues and identified 128,148 unigenes, 100% of which have been annotated. By using Digital Gene Expression (DGE) sequencing, we screened 19, 110, 289, and 211 differentially expressed genes (DEGs) in the hypothalamus, pituitary gland, stroma ovarii, and follicles, respectively, between laying and nesting geese. Expression changes of hypocretin (HCRT) and pro-opiomelanocortin (POMC) in the hypothalamus of nesting geese may cause appetite reduction, which is possibly the first step and a prerequisite to initiate broodiness. In addition to prolactin (PRL), follicle-stimulating hormone (FSH) and luteinizing hormone (LH), genes including oxytocin-neurophysin (OXT), chordin-like protein 1 (CHRDL1) and growth hormone (GH), expressed in the pituitary gland, are new candidate molecules that may be involved in broodiness in geese. Heme oxygenase 1 (HMOX1) in the pituitary gland, the proto-oncogene c-Fos (FOS), heat shock protein 90-alpha (HSP90AA), and cyclin-dependent kinase 1 (CDK1) in the ovary that may consolidate and transduce signals regulating the HPGA during broodiness in geese.
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PMID:Transcriptome analysis revealed the possible regulatory pathways initiating female geese broodiness within the hypothalamic-pituitary-gonadal axis. 2940 59


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