Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P01189 (
beta-endorphin
)
21,003
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We examined by immunocytochemistry the localization of cathepsins B and H in corticotrophs and melanotrophs in anterior and intermediate lobes of rat pituitary gland, using monospecific antibodies to cathepsins B and H. In serial semithin sections, immunodeposits for
cathepsin H
were detected throughout the cytoplasm of cells immunoreactive for ACTH and
alpha-MSH
in anterior and intermediate pituitary. Granular immunodeposits for cathepsin B were demonstrated in anterior and intermediate cells. Double immunostaining colocalized immunogold particles for
cathepsin H
and ACTH or
alpha-MSH
in secretory granules of corticotrophs or melanotrophs, whereas those for cathepsin B were detected only in their lysosomes. Enzyme assay demonstrated cathepsin B activity in both anterior and intermediate pituitary tissue, but did not detect
cathepsin H
activity in the intermediate pituitary. Western blotting, however, revealed the presence of
cathepsin H
and cystatin beta in intermediate pituitary. These results suggest that cathepsin B plays a role in protein degradation in lysosomes of corticotrophs and melanotrophs. Moreover, the presence of
cathepsin H
in secretory granules of the cells may indicate that the enzyme participates in the activation of secretory products.
...
PMID:Immunocytochemical localization of cathepsins B and H in corticotrophs and melanotrophs of rat pituitary gland. 215 33
