Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P01189 (beta-endorphin)
 21,003 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Of three casein phosphatases isolated from the cytosol of human cord blood erythrocytes two were cobalt-dependent, E2 and E3. In the presence of CoCl2, E2 activity was the most prominent. In addition to casein, E2 dephosphorylated phosvitin and p-nitrophenyl phosphate (p-NPP) with pH optima at 6.8-7.2 for proteins and 9.0 for p-NPP. The native enzyme had a molecular weight of 104,000 daltons after AcA-44 Ultrogel filtration. According to SDS/PAGE it consisted of two subunits, 78,000 and 15,000 daltons. The 104,000-dalton form exhibited Michaelis-Menten kinetics and had the greatest affinity for casein between protein substrates tested. Ethanol denaturated the enzyme by 80%. Optimal activation of E2 phosphatase was achieved with 5 mmol/l CoCl2 which did not affect the catalytic properties of the enzyme but did affect the rate of 'E-S' complex formation. Inorganic pyrophosphate was not inhibitory for the 104,000-dalton enzyme. Judging by all these properties the natural substrate for E2 casein phosphatase could be P-pyruvate kinase.
 ...
PMID:Cobalt-dependent protein phosphatases from human cord blood erythrocytes. II. Further characterization of E2 casein phosphatase. 283 85

The intraperitoneal administration of corticotropin (ACTH) in the rate of 1 and 2 units per 100 g of body weight and that of hydrocortisone in the rate of 1 mg and 5 mg per 100 g body weight were studied for their effects on carbohydrate metabolism rate in musculus gastrocnemius as well as on the level of 11-oxycorticosteroids in blood plasma of rats. The glycogen level in muscles was found to rise 3 hours after ACTH and hydrocortisone administration and it correlated with the hydrocortisone level increase in blood plasma (r = 0.714 and 0.863, respectively); the activity of pyruvate kinase decreased. Simultaneously ACTH did not change while hydrocortisone lowered the phosphorylase activity and the content of both fructose-6-phosphate and lactate.
 ...
PMID:[Effect of corticotropin and hydrocortisone on carbohydrate metabolism in skeletal muscles]. 283 20