Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01189 (beta-endorphin)
 21,003 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Release of alpha-MSH from male rat hypothalamic slices was studied using a sensitive bioassay (1-2 pg). Addition of 60 mM KCl to superfusion medium resulted in a twofold increase in alpha-MSH release compared to spontaneous release. Both spontaneous and potassium-induced release were inhibited in a dose-response manner by the tripeptide Pro-Leu-Gly-NH2 (PLG, or MIF-1); 0.04 microgram to 1 microgram PLG inhibited the alpha-MSH release but the lowest dose demonstrated a greater inhibitory effect; high concentrations of PLG, on the other hand, did not modify either spontaneous or potassium-evoked alpha-MSH release from the slices. Contrarily, DA did not modify either spontaneous or potassium- induced alpha-MSH release at any of the doses tested. These findings demonstrate that the inhibitory behavior of PLG and DA in the central nervous system (CNS) differs from their behavior towards alpha-MSH release in the pituitary. This suggests differences in the regulation of alpha-MSH release from the pituitary and the CNS.
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PMID:Inhibition by L-prolyl-L-leucyl-glycinamide (PLG) of alpha-melanocyte stimulating hormone release from hypothalamic slices. 613 63

We have investigated the effect of blocking alpha-MSH on the release of LH in rats at pro-oestrus. Continuous infusion of Pro-Leu-Gly-NH2 (PLG; 0.2 microgram/h), from the afternoon of dioestrus-2 until the day of oestrus, inhibited LH at pro-oestrus and the next ovulation on the day of oestrus. Plasma alpha-MSH levels decreased significantly in rats treated with PLG. This inhibitory effect on LH release was not observed in rats treated with a continuous infusion of a diluted antiserum against alpha-MSH (0.5 microliter/h, diluted 1/10) from the afternoon of dioestrus-2 until the day of oestrus. On the contrary, the administration of undiluted antiserum against alpha-MSH (0.5 microliter/h) blocked LH release and ovulation. The release of alpha-MSH into the blood was also significantly blocked by this treatment. When serum levels of progesterone were measured at 12.00 and 14.00 h in rats in which alpha-MSH release was blocked by PLG or concentrated antiserum, a significant decrease was observed when compared with the levels of progesterone in control rats. These results show that the blockade of alpha-MSH inhibits LH release and the next ovulation in rats treated with PLG or with concentrated antiserum against alpha-MSH, through a blockade of progesterone of adrenal origin.
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PMID:The effect of the blockade of alpha-melanocyte-stimulating hormone on LH release in the rat. 810 Aug 49