Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01185 (vasopressin)
 23,126 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Copper(II) complexes of oxytocin, 4-Glu-oxytocin, 5-Asp-oxytocin, and GlyGlyGly-Lys8-vasopressin were studied by potentiometric, EPR, and UV-visible spectroscopic methods. The formation of 4N-coordinated complexes was characteristic of all ligands. This type of coordination is especially favored for oxytocin due to the specific conformation of the ring coupled by the disulfide bridge. The coordination of the gamma-carboxylate group of 4-Glu-oxytocin and a disulfide sulfur atom of GlyGlyGly-Lys8-vasopressin was reported to occur in the 2N-complexes over medium pH range.
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PMID:Potentiometric and spectroscopic studies on the copper(II) complexes of peptide hormones containing disulfide bridges. 759 72

Our previous study demonstrated an increase in tyrosine protein phosphorylation and phosphatidylinositol phosphorylation induced by oxidants, suggesting a proliferative potential for these reactive substances. In the present study, we focus our investigation on the similarity between a redox cycling naphthoquinone and orthovanadate (VO), an oxidant generator as well as a potent phosphotyrosyl phosphatase inhibitor, in growth of 3T3-L1 cells cultured in serum-free media. Vanadate increased [3H]thymidine incorporation in a concentration-dependent manner. However, in the presence of insulin, epidermal growth factor, or platelet-derived growth factor, VO synergistically augmented, by as much as fivefold, DNA synthesis stimulated by these growth factors. An increase in the association of PI 3-kinase with the insulin receptor was also observed in cells treated with insulin + VO. Expression of the c-fos gene, a marker of early nuclear event in mitogenesis induced by insulin, EGF, and PDGF, but not vasopressin, a non-tyrosine kinase-linked mitogen, was also enhanced by VO. Flow cytometric analysis indicated an acceleration of cell cycle progression when VO and insulin were present simultaneously. Striking similarity was observed between VO and 2,3-dimethoxy-1,4-naphthoquinone (DMNQ) in PI 3-kinase activation, c-fos proto-oncogene expression, and DNA synthesis, key events associated with cell growth. Additionally, both VO and DMNQ gave rise to DMPO-.OH adduct EPR signal measured with cell suspensions. These data support an oxidant-mediated increase in tyrosine protein phosphorylation early in the signal transduction cascade of growth factor receptors, leading to augmentation of cell proliferation.
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PMID:Orthovanadate and 2,3-dimethoxy-1,4-naphthoquinone augment growth factor-induced cell proliferation and c-fos gene expression in 3T3-L1 cells. 839 47

A new vasopressin analogue, [His1,6]AVP, was synthesized and characterized by potentiometric measurements as well as by UV-Vis, CD and EPR spectroscopy. At the physiological pH the peptide forms a stable complex with Cu2+ ions which is characterized by the {NH2, NIm, NIm(macrochelate)} binding mode. The replacement of both Cys by His residues in the vasopressin sequence results in a very significant increase in the efficiency of Cu2+ binding.
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PMID:The unusual binding abilities of the His-analogue of Arg-vasopressin towards Cu2+. 1880 8