Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01185 (vasopressin)
 23,126 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A sensitive and specific radioimmunoassay has been developed capable of measuring thyrotropin releasing hormone (TRH) in extracted human plasma and urine. All of three TRH analogues tested had little cross-reactivity to antibody. Luteinizing hormone releasing hormone, lysine vasopressin, rat growth hormone and bovine albumin were without effect, but rat hypothalamic extract produced a displacement curve which was parallel to that obtained with the synthetic TRH. Sensitivity of the radioimmunoassay was 4 pg per tube with intraassay coefficient of variation of 6.2-9.7%. Synthetic TRH could be quantitatively extracted by methanol when added to human plasma in concentration of 25, 50 and 100 pg/ml. TRH immunoreactivity was rapidly reduced in plasma at 20 degrees C than at 0 degrees C, but addition of peptidase inhibitors, FOY-007 and BAL, prevented the inactivation of TRH for 3 hr at 0 degrees C. The TRH in urine was more stable at 0 degrees C than 20 degrees C, and recovered 75 +/- 4.6% hr after being added. The plasma levels of TRH were 19 pg/ml or less in normal adults and no sex difference was observed. The rate of disappearance of TRH administered i.v. from the blood could be represented as half-times of 4-12 min. Between 5.3-12.3% of the injected dose was excreted into urine within 1 hr as an immunoreactive TRH. These results indicate the usefulness of TRH radioimmunoassay for clinical investigation.
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PMID:Radioimmunoassay of thyrotropin releasing hormone in plasma and urine. 81 57

The physiological regulation of the plasma corticosteroid concentration, measured by competitive protein-binding, was studied in female rhesus monkeys (M. mulatta) sedated with phencyclidine hydrochloride. Morning basal levels of plasma corticosteroids were found to be in the range 8-0-25-2 mug/100 ml, which is lower than that previously reported in this species. A circadian rhythm in plasma cortisol concentration was demonstrated. Prolonged sedation with phencyclidine was associated with a gradual increase in the plasma cortisol concentration. Synthetic alpha1-24 adrenocorticotrophic hormone given intravenously caused a rapid rise in plasma cortisol, the minimum effective dose was between 1 and 10 ng/kg body weight and the response was maximal after 1000 ng/kg. The administration of lysine-vasopressin and the induction of hypoglycaemia by insulin were both followed by an increase in the plasma corticosteroid concentration. Metyrapone caused a decline in plasma 11-hydroxycorticosteroids and a concomitant increase in total corticosteroids measured by competitive protein-binding. It is concluded that the hypothalamic-pituitary-adrenal system in the rhesus monkey functions in a manner which is qualitatively and quantitatively similar to that of man.
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PMID:Studies on plasma corticosteroids in the rhesus monkey (Macaca mulatta). 81 35

This study deals with the effect of vasopressin on the ability to learn (short- and long-term memory) a noxious significance. The literature shows that the response to stress is always accompanied by increased vasopressin secretion. The animals used were 27 adult male rats of which 12 injected with lysine-vasopressin and 15 controls. The effect of the drug was studied on unconditioned avoidance behaviour (light-avoidance) and on learning and retention of an environment the noxious significance of which the animals had trained in a single session. Vasopressin proved to alter, as an immediate effect, the avoidance responsiveness in experimental animals.
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PMID:The effect of lysine-vasopressin upon short-term recall of a noxious significance. 81 62

The prolactin (PRL)-releasing activity of porcine stalk median eminence (pSME) was characterized by an in vivo bioassay and concomitant radioi-munoassay of plasma PRL and thyrotropin (TSH) levels. Methanol extracts of pSME stimulated PRL release in 3-day estrogen-primed rats when administered by the intracarotid route in doses ranging from 0.1 to 2.0 pSME equivalents. Synthetic thyrotropin-releasing hormone (TRH) stimulated the release of PRL and TSH in the dose range of 10 to 300 ng. PRL release was greater in response to a maximally effective dose of pSME than the release elicited by a maximal dose of TRH, and pSME administered together with a greater than mazimally effective dose of TRH caused additional PRL but not TSH secretion. Lysine vasopressin and prostaglandin E1 and E2 stimulated PRL release only at doses several orders of magnitude greater than the dose present in pSME. Somatostatin inhibited the release of TSH but not that of PRL whether the stimulus employed was pSME or TRH. The effective inhibitory dose of somatostatin was also significantly greater than the reported hypothalamic content. When pSME was subjected to incubation with plasma, a treatment reported to inactivate TRH, TSH-releasing activity was destroyed to a greater extent than was PRL-releasing activity. When pSME was adsorbed onto charcoal, the supernatant solution was devoid of TRH, as determined by complete removal of a [3H]TRH marker, yet substantial PRL-releasing activity was retained. TSH-releasing activity eluted from the charcoal with methanol was considerably greater than that expected on the basis of the recovery of [3H]TRH, suggesting the presence in the crude extract of a TSH-release inhibitor or of a TSH-releasing factor other than TRH. Based on the above evidence, we conclude that crude pSME contains PRL-releasing substance(s) distinct from the tripeptide TRH.
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PMID:Dissociation of prolactin-releasing activity from thyrotropin-releasing hormone in porcine stalk median eminence. 81 52

In this study, lysine-vasopressin, administered either as a continuous, intravenous infusion (1 unit/kh/hour) or as a selective infusion into the superior mesenteric artery (0.2 unit/minute), produced equal (25%), significant (p less than 0.05), and sustained (60 minute) reductions in portal pressure. Compared to intravenous administration, selective intra-arterial infusion of vasopressin resulted in similar reductions incardiac output (38%), myocardial contractility (23%), and coronary flow (53%). Since these adverse cardiodynamic effects were not avoided by selective intra-arterial infusion, it would appear that administration of vasopressin as a continuous infusion through a peripheral vein remains the most rapid and practical method of administering the drug.
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PMID:Adverse cardiodynamic effects of vasopressin not avoided by selective intra-arterial administration. 86 Feb 2

Transumbilical portal venous catheterization was performed in 11 patients for diagnostic selective portography. By use of a multiple-catheter technique, the effects of intravenous infusion of [8-lysine]vasopressin on portal venous mixing of tributary flows was measured by simultaneous blood sampling from the left and right portal branch, taking advantage of the oxygen saturation difference between splenic and superior mesenteric venous blood and also by infusion of 133Xe into one of the portal tributaries. Slight signs of incomplete portal mixing were observed at rest in eight of nine patients. During vasopressin infusion, the heterogeneity of oxygen saturation and xenon activity in the portal branches increased significantly. There was also a significant average shift of splenic venous blood toward the left portal branch with, in some patients, only splenic blood in this branch. Streamlining thus was a dynamic phenomenon related to the reduced and altered portal venous flow pattern. This must be considered during flow measurements, and might also have pathophysiological significance in liver diseases.
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PMID:Effect of vasopressin on the mixing of portal venous blood in awake man. 87 36

The conformation of the CCSSCC moiety in oxytocin and lysine vasopressin is investigated using laser Raman spectroscopy. The Raman spectra of solutions of these hormones in water and in dimethyl sulfoxide show an intense band at 508 cm-1 which is assigned to the S-S stretching mode. The presence of shoulders on this band between 490 and 525 cm-1 shows that there is an equilibrium among several conformations for the disulfide unit of these hormones in solution. Most of the CS-SC dihedral angles are within 30 degrees of +/-90 degrees, but some of the molecules have CS-SC dihedral angles strained away from this value by more than 30 degrees. The previously published circular dichroism spectra of these hormones are reinterpreted, and it is shown that the circular dichroism spectra indicate the presence of more than one conformation for the disulfide unit, in agreement with the Raman results.
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PMID:A Raman spectroscopic investigation of the disulfide conformation in oxytocin and lysine vasopressin. 91 68

Responses to exogenous norepinephrine (NE), transmural electrical stimulation, 5-hydroxytryptamine (5-HT) and lysine vasopressin were studied in isolated helical strips of the small artery and vein from the mesoappendix of patients undergoing incidental appendectomy at the time of cholecystectomy. Responses to NE and 5-HT were similar in each vessel. Arterial strips were unresponsive to electrical stimulation and responses to vasopressin were greater than those to NE in this tissue. Venous strips were unresponsive to vasopressin. Relaxation of exogenous NE responses following oil immersion of arterial strips was unaffected by cocaine whereas relaxation of similarly treated venous strips was markedly prolonged. The data suggest: (1) that the artery of human mesoappendix is poorly innervated and (2) that vasopressin is clearly more active on human mesoappendix artery than it is on human mexoappendix vein. The latter observation may help to explain the efficacy of vasopressin infusion in gastrointestinal hemorrhage secondary to portal hypertension.
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PMID:Differential responses to transmural stimulation and vasopressin in isolated strips of artery and vein of human mesoappendix. 91 99

Rabbits immunized with lysine-vasopressin bovine serum albumin conjugate showed the diabetes insipidus syndrome intermittently manifested by polyuria and polydipsia with various degrees of peaking from the eighth to fourteenth day post injection during boosters. The antibody-antidiuretic hormone immune complexes which may interfere with the action of the endogenous vasopressin on the kidney were found in the diabetes insipidus rabbits. However, the degree of the polyuria was not necessarily related to the quantities of the formed immune complexes, the titer, nor the affinity of the antiserum. It is suggested that the degree of the polyuria is related not only to the binding ability of the antiserum to the endogeneous vasopressin, but also to other factors.
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PMID:Immune complexes in diabetes insipidus syndrome of rabbits immunized with vasopressin. 91 33

1-Deamino-4-L-valine-8-DL-homolysine-vasopressin and protected 1-deamino-4-l-valine-8-D-lysine-vasopressin were synthesized by the solid phase method and were then converted into the title compounds (dVDHLVP and dVDHAVP) by tryptic digestion and epsilon-guanidination, respectively. The new hormone analogues exhibit only moderate antidiuretic potency, dVDHLVP 21 units/mg and dVDHAVP 31 units/mg, but since they are essentially devoid of pressor activity (o.o1 units/mg/ the A/P ratios are very high. In fact, dVDHLVP is the most specific antidiuretic agent in the lysine series known so far.
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PMID:Solid phase synthesis and some hormonal activities of 1-deamino-4-L-valine-8-D-homolysine-and 1-deamino-4-L-valine-8-D-homoarginine-vasopressin. 91 27


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